Diaphanization: A Standardized Protocol for Non-Fetal Tissue Preservation

In response to the arising difficulty of dissection use during anatomy courses, medical schools have been forced to research alternative teaching methods. These are meant to help students develop three dimensional mental images of the human body and increase spatial reasoning, thus improving the lea...

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Autores principales:	Rueda-Esteban,Roberto Javier, Palacio Varona,Juliana, López-McCormick,Juan Sebastián, Hernández Restrepo,Juan David
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2017
Materias:	Teaching of anatomy Diaphanization Preservation of Adult Tissue Standardized protocol
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000200027
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spelling	oai:scielo:S0717-950220170002000272017-08-07Diaphanization: A Standardized Protocol for Non-Fetal Tissue PreservationRueda-Esteban,Roberto JavierPalacio Varona,JulianaLópez-McCormick,Juan SebastiánHernández Restrepo,Juan David Teaching of anatomy Diaphanization Preservation of Adult Tissue Standardized protocol In response to the arising difficulty of dissection use during anatomy courses, medical schools have been forced to research alternative teaching methods. These are meant to help students develop three dimensional mental images of the human body and increase spatial reasoning, thus improving the learning process of human morphology. One of those methods, used at the Universidad de los Andes is the Diaphanization process. This technique is a well-known method for specimen preservation, used as an anatomy research and teaching tool. Even though it is frequently used, finding a standardized protocol in indexed journals is not possible, which hinders the use of the technique. The standardization of an updated protocol is a need in order to continue exploiting the maximum educational capacity of the specimens used during classwork. The process used at the Universidad de los Andes for non-fetal tissue preservation by Diaphanization is described, with a detailed explanation of its five main stages: Injection, Fixation, Dehydration, Bleaching (or Maceration) and Final preservation. The final result should be an organ preserved in an acrylic box which allows clear three dimensional visualization of anatomical structures that can be used for the study and description of general anatomy and vascular structure. It also helps with spatial reasoning and represents little to no biological risk, leading to a new level of anatomical teaching and experimenting. Several specimens obtained at our laboratory through this technique are presented.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.35 n.2 20172017-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000200027en10.4067/S0717-95022017000200027
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Teaching of anatomy Diaphanization Preservation of Adult Tissue Standardized protocol
spellingShingle	Teaching of anatomy Diaphanization Preservation of Adult Tissue Standardized protocol Rueda-Esteban,Roberto Javier Palacio Varona,Juliana López-McCormick,Juan Sebastián Hernández Restrepo,Juan David Diaphanization: A Standardized Protocol for Non-Fetal Tissue Preservation
description	In response to the arising difficulty of dissection use during anatomy courses, medical schools have been forced to research alternative teaching methods. These are meant to help students develop three dimensional mental images of the human body and increase spatial reasoning, thus improving the learning process of human morphology. One of those methods, used at the Universidad de los Andes is the Diaphanization process. This technique is a well-known method for specimen preservation, used as an anatomy research and teaching tool. Even though it is frequently used, finding a standardized protocol in indexed journals is not possible, which hinders the use of the technique. The standardization of an updated protocol is a need in order to continue exploiting the maximum educational capacity of the specimens used during classwork. The process used at the Universidad de los Andes for non-fetal tissue preservation by Diaphanization is described, with a detailed explanation of its five main stages: Injection, Fixation, Dehydration, Bleaching (or Maceration) and Final preservation. The final result should be an organ preserved in an acrylic box which allows clear three dimensional visualization of anatomical structures that can be used for the study and description of general anatomy and vascular structure. It also helps with spatial reasoning and represents little to no biological risk, leading to a new level of anatomical teaching and experimenting. Several specimens obtained at our laboratory through this technique are presented.
author	Rueda-Esteban,Roberto Javier Palacio Varona,Juliana López-McCormick,Juan Sebastián Hernández Restrepo,Juan David
author_facet	Rueda-Esteban,Roberto Javier Palacio Varona,Juliana López-McCormick,Juan Sebastián Hernández Restrepo,Juan David
author_sort	Rueda-Esteban,Roberto Javier
title	Diaphanization: A Standardized Protocol for Non-Fetal Tissue Preservation
title_short	Diaphanization: A Standardized Protocol for Non-Fetal Tissue Preservation
title_full	Diaphanization: A Standardized Protocol for Non-Fetal Tissue Preservation
title_fullStr	Diaphanization: A Standardized Protocol for Non-Fetal Tissue Preservation
title_full_unstemmed	Diaphanization: A Standardized Protocol for Non-Fetal Tissue Preservation
title_sort	diaphanization: a standardized protocol for non-fetal tissue preservation
publisher	Sociedad Chilena de Anatomía
publishDate	2017
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000200027
work_keys_str_mv	AT ruedaestebanrobertojavier diaphanizationastandardizedprotocolfornonfetaltissuepreservation AT palaciovaronajuliana diaphanizationastandardizedprotocolfornonfetaltissuepreservation AT lopezmccormickjuansebastian diaphanizationastandardizedprotocolfornonfetaltissuepreservation AT hernandezrestrepojuandavid diaphanizationastandardizedprotocolfornonfetaltissuepreservation
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Diaphanization: A Standardized Protocol for Non-Fetal Tissue Preservation

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