Modulation of in vitro Osteoclast Activity Mediated by Different Doses of Parathyroid Hormone

Bone remodeling is a process regulated by the interaction between cells and various molecules such as parathyroid hormone (PTH). The aim of the study was to evaluate the effect of different doses of PTH on osteoclast activity in a culture model of bone organs. Six-day-old male C57BL/6 mice (n=14) we...

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Autores principales:	Ginani,Fernanda, Mota-Filho,Haroldo Gurgel, Vasconcelos Barreto,Mardem Portela e, Barboza,Carlos Augusto Galvão
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2017
Materias:	Parathyroid hormone Osteoclast Bone remodeling Organ culture techniques
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000200033
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spelling	oai:scielo:S0717-950220170002000332017-08-07Modulation of in vitro Osteoclast Activity Mediated by Different Doses of Parathyroid HormoneGinani,FernandaMota-Filho,Haroldo GurgelVasconcelos Barreto,Mardem Portela eBarboza,Carlos Augusto Galvão Parathyroid hormone Osteoclast Bone remodeling Organ culture techniques Bone remodeling is a process regulated by the interaction between cells and various molecules such as parathyroid hormone (PTH). The aim of the study was to evaluate the effect of different doses of PTH on osteoclast activity in a culture model of bone organs. Six-day-old male C57BL/6 mice (n=14) were euthanized and the calvariae were dissected and sectioned in the middle, keeping the periosteal and endosteal. The bone fragments were divided into three groups: Group I (control - without adding PTH), Group II (addition of 3 nM PTH) and Group III (30 nM PTH), all cultured in aMEM for up to 72 h osteoclast activity was evaluated by biochemical quantification of calcium released in the culture medium at intervals of 24, 48, and 72 h and by histomorphometric analysis of bone resorption lacunae at 72 h our results show that group II exhibited significantly higher values of calcium levels in the medium compared to group I (p<0.05) in all intervals, also being higher for group III at 24 hours (p<0.05). Group II promoted a greater demineralization area (22068 ± 2193 mm2) than those found in group I (2084 ± 38 mm2) and group III (8952 ± 246 mm2), with statistically significant difference (p<0.001) among all groups. We concluded that in culture model of bone organs PTH promotes higher bone resorption when administered in lower doses.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.35 n.2 20172017-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000200033en10.4067/S0717-95022017000200033
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Parathyroid hormone Osteoclast Bone remodeling Organ culture techniques
spellingShingle	Parathyroid hormone Osteoclast Bone remodeling Organ culture techniques Ginani,Fernanda Mota-Filho,Haroldo Gurgel Vasconcelos Barreto,Mardem Portela e Barboza,Carlos Augusto Galvão Modulation of in vitro Osteoclast Activity Mediated by Different Doses of Parathyroid Hormone
description	Bone remodeling is a process regulated by the interaction between cells and various molecules such as parathyroid hormone (PTH). The aim of the study was to evaluate the effect of different doses of PTH on osteoclast activity in a culture model of bone organs. Six-day-old male C57BL/6 mice (n=14) were euthanized and the calvariae were dissected and sectioned in the middle, keeping the periosteal and endosteal. The bone fragments were divided into three groups: Group I (control - without adding PTH), Group II (addition of 3 nM PTH) and Group III (30 nM PTH), all cultured in aMEM for up to 72 h osteoclast activity was evaluated by biochemical quantification of calcium released in the culture medium at intervals of 24, 48, and 72 h and by histomorphometric analysis of bone resorption lacunae at 72 h our results show that group II exhibited significantly higher values of calcium levels in the medium compared to group I (p<0.05) in all intervals, also being higher for group III at 24 hours (p<0.05). Group II promoted a greater demineralization area (22068 ± 2193 mm2) than those found in group I (2084 ± 38 mm2) and group III (8952 ± 246 mm2), with statistically significant difference (p<0.001) among all groups. We concluded that in culture model of bone organs PTH promotes higher bone resorption when administered in lower doses.
author	Ginani,Fernanda Mota-Filho,Haroldo Gurgel Vasconcelos Barreto,Mardem Portela e Barboza,Carlos Augusto Galvão
author_facet	Ginani,Fernanda Mota-Filho,Haroldo Gurgel Vasconcelos Barreto,Mardem Portela e Barboza,Carlos Augusto Galvão
author_sort	Ginani,Fernanda
title	Modulation of in vitro Osteoclast Activity Mediated by Different Doses of Parathyroid Hormone
title_short	Modulation of in vitro Osteoclast Activity Mediated by Different Doses of Parathyroid Hormone
title_full	Modulation of in vitro Osteoclast Activity Mediated by Different Doses of Parathyroid Hormone
title_fullStr	Modulation of in vitro Osteoclast Activity Mediated by Different Doses of Parathyroid Hormone
title_full_unstemmed	Modulation of in vitro Osteoclast Activity Mediated by Different Doses of Parathyroid Hormone
title_sort	modulation of in vitro osteoclast activity mediated by different doses of parathyroid hormone
publisher	Sociedad Chilena de Anatomía
publishDate	2017
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000200033
work_keys_str_mv	AT ginanifernanda modulationofinvitroosteoclastactivitymediatedbydifferentdosesofparathyroidhormone AT motafilhoharoldogurgel modulationofinvitroosteoclastactivitymediatedbydifferentdosesofparathyroidhormone AT vasconcelosbarretomardemportelae modulationofinvitroosteoclastactivitymediatedbydifferentdosesofparathyroidhormone AT barbozacarlosaugustogalvao modulationofinvitroosteoclastactivitymediatedbydifferentdosesofparathyroidhormone
_version_	1718445004123275264

Modulation of in vitro Osteoclast Activity Mediated by Different Doses of Parathyroid Hormone

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