Leptin and Leptin Receptor are Expressed only in Clear Cells of Rat Epididymis Epithelia

SUMMARY: Leptin is a 16 kilodaltons hormone secreted by adipose tissue and in the past few years it has been related to the reproductive system regulation. Leptin and its receptor (OBR) have been described in several reproductive organs and in different species but, in epididymis, there is still a l...

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Autores principales:	Gombar,Flavia Meireles, Ramos,Cristiane da Fonte
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2017
Materias:	Epididymis Clear cell Leptin OBR
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000401303
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spelling	oai:scielo:S0717-950220170004013032019-09-16Leptin and Leptin Receptor are Expressed only in Clear Cells of Rat Epididymis EpitheliaGombar,Flavia MeirelesRamos,Cristiane da Fonte Epididymis Clear cell Leptin OBR SUMMARY: Leptin is a 16 kilodaltons hormone secreted by adipose tissue and in the past few years it has been related to the reproductive system regulation. Leptin and its receptor (OBR) have been described in several reproductive organs and in different species but, in epididymis, there is still a lack of information. The aim of this work is to establish if leptin and its receptor are present on epididymis and where the production is occurring. At mRNA level the cauda portion showed a high expression of leptin (p<0.025) and OBRa (p<0.002) while at protein level the OBR expression was lower in cauda region (p<0.025) and leptin was not detected. The ratio between OBRa and OBRb was higher in both regions despite its total amount. By immunohistochemistry leptin and OBR were detected on epididymis epithelia, restricted to clear cells (CC). After efferent duct ligation (EDL) a decrease on leptin staining on CC was observed, suggesting that despite of epididymis production, most of leptin source may probably come from testis. Our results show that leptin and OBR, both mRNA and protein, are present on epididymis and exclusively in CC, suggesting that this tissue is responsive to the hormone and may have an important role on CC regulation.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.35 n.4 20172017-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000401303en10.4067/S0717-95022017000401303
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Epididymis Clear cell Leptin OBR
spellingShingle	Epididymis Clear cell Leptin OBR Gombar,Flavia Meireles Ramos,Cristiane da Fonte Leptin and Leptin Receptor are Expressed only in Clear Cells of Rat Epididymis Epithelia
description	SUMMARY: Leptin is a 16 kilodaltons hormone secreted by adipose tissue and in the past few years it has been related to the reproductive system regulation. Leptin and its receptor (OBR) have been described in several reproductive organs and in different species but, in epididymis, there is still a lack of information. The aim of this work is to establish if leptin and its receptor are present on epididymis and where the production is occurring. At mRNA level the cauda portion showed a high expression of leptin (p<0.025) and OBRa (p<0.002) while at protein level the OBR expression was lower in cauda region (p<0.025) and leptin was not detected. The ratio between OBRa and OBRb was higher in both regions despite its total amount. By immunohistochemistry leptin and OBR were detected on epididymis epithelia, restricted to clear cells (CC). After efferent duct ligation (EDL) a decrease on leptin staining on CC was observed, suggesting that despite of epididymis production, most of leptin source may probably come from testis. Our results show that leptin and OBR, both mRNA and protein, are present on epididymis and exclusively in CC, suggesting that this tissue is responsive to the hormone and may have an important role on CC regulation.
author	Gombar,Flavia Meireles Ramos,Cristiane da Fonte
author_facet	Gombar,Flavia Meireles Ramos,Cristiane da Fonte
author_sort	Gombar,Flavia Meireles
title	Leptin and Leptin Receptor are Expressed only in Clear Cells of Rat Epididymis Epithelia
title_short	Leptin and Leptin Receptor are Expressed only in Clear Cells of Rat Epididymis Epithelia
title_full	Leptin and Leptin Receptor are Expressed only in Clear Cells of Rat Epididymis Epithelia
title_fullStr	Leptin and Leptin Receptor are Expressed only in Clear Cells of Rat Epididymis Epithelia
title_full_unstemmed	Leptin and Leptin Receptor are Expressed only in Clear Cells of Rat Epididymis Epithelia
title_sort	leptin and leptin receptor are expressed only in clear cells of rat epididymis epithelia
publisher	Sociedad Chilena de Anatomía
publishDate	2017
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000401303
work_keys_str_mv	AT gombarflaviameireles leptinandleptinreceptorareexpressedonlyinclearcellsofratepididymisepithelia AT ramoscristianedafonte leptinandleptinreceptorareexpressedonlyinclearcellsofratepididymisepithelia
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Leptin and Leptin Receptor are Expressed only in Clear Cells of Rat Epididymis Epithelia

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