Development of a Rat Model of Knee Osteoarthritis by a Combination of Monoiodoacetate and Streptozotocin

SUMMARY: Osteoarthritis (OA) caused by ageing joints or as a secondary complication of diabetes is a common health problem. We sought to develop an animal model of OA induced by a combination of the chondrocyte glycolytic inhibitor mono-iodoacetate (MIA) and streptozotocin (STZ), the agent that indu...

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Autores principales:	Heidar,El Hassan A., Al-Ani,Bahjat, Haidara,Mohamed A, Al-Faya,Fareed F, Al-Humayed,Suliman, Eid,Refaat A, Hassan,Waleed N.
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2017
Materias:	Knee osteoarthritis Diabetes Monoiodoacetate Rat model Cartilage ultrastructure
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000401383
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spelling	oai:scielo:S0717-950220170004013832019-09-16Development of a Rat Model of Knee Osteoarthritis by a Combination of Monoiodoacetate and StreptozotocinHeidar,El Hassan A.Al-Ani,BahjatHaidara,Mohamed AAl-Faya,Fareed FAl-Humayed,SulimanEid,Refaat AHassan,Waleed N. Knee osteoarthritis Diabetes Monoiodoacetate Rat model Cartilage ultrastructure SUMMARY: Osteoarthritis (OA) caused by ageing joints or as a secondary complication of diabetes is a common health problem. We sought to develop an animal model of OA induced by a combination of the chondrocyte glycolytic inhibitor mono-iodoacetate (MIA) and streptozotocin (STZ), the agent that induces diabetes mellitus. We then hypothesized that the extent of damages to the knee joint induced by this model can be greater than OA induced by either MIA or STZ. Rats were either injected with MIA (model 1) or STZ (model 2) or both agents (model 3). After 8 weeks, harvested tissues from the knee joint of these groups were examined using scanning and transmission electron microscopy. In addition, blood samples were assayed for tumor necrosis factor alpha (TNF-α) and interleukin -6 (IL-6) that are known to be modulated in OA and diabetes. Compared to control group, substantial damages to the articular cartilage of the knee joint were observed in the three models with the severest in model 3. In addition, rats in model 3 showed significant (P<0.0001) increase in TNF-α and IL-6 compared to model 1 and 2. Thus, we have developed a new model of knee OA in rats that mimics a type of OA that is common among elderly people who have both, “ageing” joints and diabetes.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.35 n.4 20172017-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000401383en10.4067/S0717-95022017000401383
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Knee osteoarthritis Diabetes Monoiodoacetate Rat model Cartilage ultrastructure
spellingShingle	Knee osteoarthritis Diabetes Monoiodoacetate Rat model Cartilage ultrastructure Heidar,El Hassan A. Al-Ani,Bahjat Haidara,Mohamed A Al-Faya,Fareed F Al-Humayed,Suliman Eid,Refaat A Hassan,Waleed N. Development of a Rat Model of Knee Osteoarthritis by a Combination of Monoiodoacetate and Streptozotocin
description	SUMMARY: Osteoarthritis (OA) caused by ageing joints or as a secondary complication of diabetes is a common health problem. We sought to develop an animal model of OA induced by a combination of the chondrocyte glycolytic inhibitor mono-iodoacetate (MIA) and streptozotocin (STZ), the agent that induces diabetes mellitus. We then hypothesized that the extent of damages to the knee joint induced by this model can be greater than OA induced by either MIA or STZ. Rats were either injected with MIA (model 1) or STZ (model 2) or both agents (model 3). After 8 weeks, harvested tissues from the knee joint of these groups were examined using scanning and transmission electron microscopy. In addition, blood samples were assayed for tumor necrosis factor alpha (TNF-α) and interleukin -6 (IL-6) that are known to be modulated in OA and diabetes. Compared to control group, substantial damages to the articular cartilage of the knee joint were observed in the three models with the severest in model 3. In addition, rats in model 3 showed significant (P<0.0001) increase in TNF-α and IL-6 compared to model 1 and 2. Thus, we have developed a new model of knee OA in rats that mimics a type of OA that is common among elderly people who have both, “ageing” joints and diabetes.
author	Heidar,El Hassan A. Al-Ani,Bahjat Haidara,Mohamed A Al-Faya,Fareed F Al-Humayed,Suliman Eid,Refaat A Hassan,Waleed N.
author_facet	Heidar,El Hassan A. Al-Ani,Bahjat Haidara,Mohamed A Al-Faya,Fareed F Al-Humayed,Suliman Eid,Refaat A Hassan,Waleed N.
author_sort	Heidar,El Hassan A.
title	Development of a Rat Model of Knee Osteoarthritis by a Combination of Monoiodoacetate and Streptozotocin
title_short	Development of a Rat Model of Knee Osteoarthritis by a Combination of Monoiodoacetate and Streptozotocin
title_full	Development of a Rat Model of Knee Osteoarthritis by a Combination of Monoiodoacetate and Streptozotocin
title_fullStr	Development of a Rat Model of Knee Osteoarthritis by a Combination of Monoiodoacetate and Streptozotocin
title_full_unstemmed	Development of a Rat Model of Knee Osteoarthritis by a Combination of Monoiodoacetate and Streptozotocin
title_sort	development of a rat model of knee osteoarthritis by a combination of monoiodoacetate and streptozotocin
publisher	Sociedad Chilena de Anatomía
publishDate	2017
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000401383
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Development of a Rat Model of Knee Osteoarthritis by a Combination of Monoiodoacetate and Streptozotocin

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