Assessment of Mouse Oocytes Ultrastructure Following Vitrification Before and After in vitro Maturation

SUMMARY: Vitrification is a physical process in which the concentrated cryoprotectant solution after exposure to extreme cold without ice crystal formation in living cells to be converted glassing state. In this study, maturation rate and ultrastructure of mouse oocytes followed by vitrification bef...

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Autores principales:	Izadi,Maryam, Eftekhar-Vaghefi,Seyed Hassan, Akbari,Hakimeh, Asadi-Shekari,Majid, Mokhtari,Tahmineh
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2018
Materias:	Vitrification Fertility preservation Cryopreservation In Vitro Maturation Ultrastructure
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022018000100180
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spelling	oai:scielo:S0717-950220180001001802019-09-16Assessment of Mouse Oocytes Ultrastructure Following Vitrification Before and After in vitro MaturationIzadi,MaryamEftekhar-Vaghefi,Seyed HassanAkbari,HakimehAsadi-Shekari,MajidMokhtari,Tahmineh Vitrification Fertility preservation Cryopreservation In Vitro Maturation Ultrastructure SUMMARY: Vitrification is a physical process in which the concentrated cryoprotectant solution after exposure to extreme cold without ice crystal formation in living cells to be converted glassing state. In this study, maturation rate and ultrastructure of mouse oocytes followed by vitrification before or after in-virto maturation (IVM) were evaluated. A total of 373 germinal vesicle oocytes were obtained from ovaries and divided into three fresh IVM, IVM vitrified, vitrified IVM groups. Ten metaphase II oocytes were obtained from uterine tubes and considered as the control group. Oocytes in vitrified groups were vitrified by Cryotop using vitrification medium and kept in liquid nitrogen. The maturation media was a-MEM supplemented with rFSH + hCG. After 24-48 h of incubation, the oocytes were investigated for nuclear maturation and ultrastructural changes using transmission electron microscopy (TEM). The oocyte maturation rate in vIVM group was significantly lower than IVMv group, when the two groups were compared with vIVM had the highest maturity. The evaluation ultrastructure of the four groups showed that the number of cortical granules, microvilli and mitochondria-SER aggregates in vIVM group were lowest and the highest amongst the number of vacuoles. Zona pellucida was darker than the control group in two freeze groups vIVM and IVMv. Most similar groups to the control group were group vIVM, Group IVMv and ultimately vIVM group, respectively. According to the results, IVM procedure is more efficient when it is performed before oocyte vitrification.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.36 n.1 20182018-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022018000100180en10.4067/S0717-95022018000100180
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Vitrification Fertility preservation Cryopreservation In Vitro Maturation Ultrastructure
spellingShingle	Vitrification Fertility preservation Cryopreservation In Vitro Maturation Ultrastructure Izadi,Maryam Eftekhar-Vaghefi,Seyed Hassan Akbari,Hakimeh Asadi-Shekari,Majid Mokhtari,Tahmineh Assessment of Mouse Oocytes Ultrastructure Following Vitrification Before and After in vitro Maturation
description	SUMMARY: Vitrification is a physical process in which the concentrated cryoprotectant solution after exposure to extreme cold without ice crystal formation in living cells to be converted glassing state. In this study, maturation rate and ultrastructure of mouse oocytes followed by vitrification before or after in-virto maturation (IVM) were evaluated. A total of 373 germinal vesicle oocytes were obtained from ovaries and divided into three fresh IVM, IVM vitrified, vitrified IVM groups. Ten metaphase II oocytes were obtained from uterine tubes and considered as the control group. Oocytes in vitrified groups were vitrified by Cryotop using vitrification medium and kept in liquid nitrogen. The maturation media was a-MEM supplemented with rFSH + hCG. After 24-48 h of incubation, the oocytes were investigated for nuclear maturation and ultrastructural changes using transmission electron microscopy (TEM). The oocyte maturation rate in vIVM group was significantly lower than IVMv group, when the two groups were compared with vIVM had the highest maturity. The evaluation ultrastructure of the four groups showed that the number of cortical granules, microvilli and mitochondria-SER aggregates in vIVM group were lowest and the highest amongst the number of vacuoles. Zona pellucida was darker than the control group in two freeze groups vIVM and IVMv. Most similar groups to the control group were group vIVM, Group IVMv and ultimately vIVM group, respectively. According to the results, IVM procedure is more efficient when it is performed before oocyte vitrification.
author	Izadi,Maryam Eftekhar-Vaghefi,Seyed Hassan Akbari,Hakimeh Asadi-Shekari,Majid Mokhtari,Tahmineh
author_facet	Izadi,Maryam Eftekhar-Vaghefi,Seyed Hassan Akbari,Hakimeh Asadi-Shekari,Majid Mokhtari,Tahmineh
author_sort	Izadi,Maryam
title	Assessment of Mouse Oocytes Ultrastructure Following Vitrification Before and After in vitro Maturation
title_short	Assessment of Mouse Oocytes Ultrastructure Following Vitrification Before and After in vitro Maturation
title_full	Assessment of Mouse Oocytes Ultrastructure Following Vitrification Before and After in vitro Maturation
title_fullStr	Assessment of Mouse Oocytes Ultrastructure Following Vitrification Before and After in vitro Maturation
title_full_unstemmed	Assessment of Mouse Oocytes Ultrastructure Following Vitrification Before and After in vitro Maturation
title_sort	assessment of mouse oocytes ultrastructure following vitrification before and after in vitro maturation
publisher	Sociedad Chilena de Anatomía
publishDate	2018
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022018000100180
work_keys_str_mv	AT izadimaryam assessmentofmouseoocytesultrastructurefollowingvitrificationbeforeandafterinvitromaturation AT eftekharvaghefiseyedhassan assessmentofmouseoocytesultrastructurefollowingvitrificationbeforeandafterinvitromaturation AT akbarihakimeh assessmentofmouseoocytesultrastructurefollowingvitrificationbeforeandafterinvitromaturation AT asadishekarimajid assessmentofmouseoocytesultrastructurefollowingvitrificationbeforeandafterinvitromaturation AT mokhtaritahmineh assessmentofmouseoocytesultrastructurefollowingvitrificationbeforeandafterinvitromaturation
_version_	1718445036282052608

Assessment of Mouse Oocytes Ultrastructure Following Vitrification Before and After in vitro Maturation

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