Conservation of Body Sections and Organs of the Narrownose Smooth-hound, Mustelus schmitti (Pisces, Chondrichthyes), by Silicone Injection at Room Temperature to be Used in Comparative Anatomy learning

SUMMARY: Comparative Anatomy deals with the study of the ontogenetic and phylogenetic changes of the vertebrates, requiring complementing the theoretical aspects with the observation of structures in specimens belonging to different taxonomic groups. The aim of the present study was to test the inje...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Popp,Albertina Inés, Basso,Ana Paula, Lodovichi,Mariela Victoria, Sidorkewicj,Nora Silvia
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2018
Materias:	Conservation techniques Acetic silicone Education Dissection Comparative Anatomy
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022018000200413
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0717-95022018000200413
record_format	dspace
spelling	oai:scielo:S0717-950220180002004132019-09-16Conservation of Body Sections and Organs of the Narrownose Smooth-hound, Mustelus schmitti (Pisces, Chondrichthyes), by Silicone Injection at Room Temperature to be Used in Comparative Anatomy learningPopp,Albertina InésBasso,Ana PaulaLodovichi,Mariela VictoriaSidorkewicj,Nora Silvia Conservation techniques Acetic silicone Education Dissection Comparative Anatomy SUMMARY: Comparative Anatomy deals with the study of the ontogenetic and phylogenetic changes of the vertebrates, requiring complementing the theoretical aspects with the observation of structures in specimens belonging to different taxonomic groups. The aim of the present study was to test the injection of silicone at room temperature in organs and trunk sections of Mustelus schmitti as an alternative to the plastination technique. Samples consisted in brain, eyes, heart, proximal end of the ventral aorta, digestive tract, spleen, pancreas, kidneys, testis and cross body section at a pre-caudal level. Material was fixed with formalin (10-5 %), dehydrated with growing concentrations of isopropyl (30 % - 50 % - 70 % - 90 % - 100 % - 100 %), impregnated with diluted commercial silicone and cured at room temperature. The whole process took 66 days. The brain was the unique organ that could not undergo the complete procedure because it did not resist the injection of silicone. The other pieces resulted in materials that characterised by being off-colour, dry, semi-flexible, lightweight, odourless, and non-toxic. They showed no signs of fungal colonization or bacterial degradation after two years of being obtained. Shrinkage was observed, which ranged among 2-25 % for total length, and from 5-26 % for maximum width (mean values: 14 and 15 %, respectively), being testicle the organ that suffered greater shrinkage in both dimensions. The degree of contraction in length and width for each of the samples was generally similar (difference £ 3 %), indicating that not striking deformation occurred. Deformation was observed only for the trunk section, eye, stomach, pancreas and valvular intestine. The technique did not affect the morphology of the structures, allowing the correct visualization of all the basic features required to recognise them. We conclude that this simple and economic method is an adequate alternative to be implemented for the conservation of small-size materials with educational purposes in Comparative Anatomy courses.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.36 n.2 20182018-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022018000200413en10.4067/S0717-95022018000200413
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Conservation techniques Acetic silicone Education Dissection Comparative Anatomy
spellingShingle	Conservation techniques Acetic silicone Education Dissection Comparative Anatomy Popp,Albertina Inés Basso,Ana Paula Lodovichi,Mariela Victoria Sidorkewicj,Nora Silvia Conservation of Body Sections and Organs of the Narrownose Smooth-hound, Mustelus schmitti (Pisces, Chondrichthyes), by Silicone Injection at Room Temperature to be Used in Comparative Anatomy learning
description	SUMMARY: Comparative Anatomy deals with the study of the ontogenetic and phylogenetic changes of the vertebrates, requiring complementing the theoretical aspects with the observation of structures in specimens belonging to different taxonomic groups. The aim of the present study was to test the injection of silicone at room temperature in organs and trunk sections of Mustelus schmitti as an alternative to the plastination technique. Samples consisted in brain, eyes, heart, proximal end of the ventral aorta, digestive tract, spleen, pancreas, kidneys, testis and cross body section at a pre-caudal level. Material was fixed with formalin (10-5 %), dehydrated with growing concentrations of isopropyl (30 % - 50 % - 70 % - 90 % - 100 % - 100 %), impregnated with diluted commercial silicone and cured at room temperature. The whole process took 66 days. The brain was the unique organ that could not undergo the complete procedure because it did not resist the injection of silicone. The other pieces resulted in materials that characterised by being off-colour, dry, semi-flexible, lightweight, odourless, and non-toxic. They showed no signs of fungal colonization or bacterial degradation after two years of being obtained. Shrinkage was observed, which ranged among 2-25 % for total length, and from 5-26 % for maximum width (mean values: 14 and 15 %, respectively), being testicle the organ that suffered greater shrinkage in both dimensions. The degree of contraction in length and width for each of the samples was generally similar (difference £ 3 %), indicating that not striking deformation occurred. Deformation was observed only for the trunk section, eye, stomach, pancreas and valvular intestine. The technique did not affect the morphology of the structures, allowing the correct visualization of all the basic features required to recognise them. We conclude that this simple and economic method is an adequate alternative to be implemented for the conservation of small-size materials with educational purposes in Comparative Anatomy courses.
author	Popp,Albertina Inés Basso,Ana Paula Lodovichi,Mariela Victoria Sidorkewicj,Nora Silvia
author_facet	Popp,Albertina Inés Basso,Ana Paula Lodovichi,Mariela Victoria Sidorkewicj,Nora Silvia
author_sort	Popp,Albertina Inés
title	Conservation of Body Sections and Organs of the Narrownose Smooth-hound, Mustelus schmitti (Pisces, Chondrichthyes), by Silicone Injection at Room Temperature to be Used in Comparative Anatomy learning
title_short	Conservation of Body Sections and Organs of the Narrownose Smooth-hound, Mustelus schmitti (Pisces, Chondrichthyes), by Silicone Injection at Room Temperature to be Used in Comparative Anatomy learning
title_full	Conservation of Body Sections and Organs of the Narrownose Smooth-hound, Mustelus schmitti (Pisces, Chondrichthyes), by Silicone Injection at Room Temperature to be Used in Comparative Anatomy learning
title_fullStr	Conservation of Body Sections and Organs of the Narrownose Smooth-hound, Mustelus schmitti (Pisces, Chondrichthyes), by Silicone Injection at Room Temperature to be Used in Comparative Anatomy learning
title_full_unstemmed	Conservation of Body Sections and Organs of the Narrownose Smooth-hound, Mustelus schmitti (Pisces, Chondrichthyes), by Silicone Injection at Room Temperature to be Used in Comparative Anatomy learning
title_sort	conservation of body sections and organs of the narrownose smooth-hound, mustelus schmitti (pisces, chondrichthyes), by silicone injection at room temperature to be used in comparative anatomy learning
publisher	Sociedad Chilena de Anatomía
publishDate	2018
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022018000200413
work_keys_str_mv	AT poppalbertinaines conservationofbodysectionsandorgansofthenarrownosesmoothhoundmustelusschmittipisceschondrichthyesbysiliconeinjectionatroomtemperaturetobeusedincomparativeanatomylearning AT bassoanapaula conservationofbodysectionsandorgansofthenarrownosesmoothhoundmustelusschmittipisceschondrichthyesbysiliconeinjectionatroomtemperaturetobeusedincomparativeanatomylearning AT lodovichimarielavictoria conservationofbodysectionsandorgansofthenarrownosesmoothhoundmustelusschmittipisceschondrichthyesbysiliconeinjectionatroomtemperaturetobeusedincomparativeanatomylearning AT sidorkewicjnorasilvia conservationofbodysectionsandorgansofthenarrownosesmoothhoundmustelusschmittipisceschondrichthyesbysiliconeinjectionatroomtemperaturetobeusedincomparativeanatomylearning
_version_	1718445042962530304

Conservation of Body Sections and Organs of the Narrownose Smooth-hound, Mustelus schmitti (Pisces, Chondrichthyes), by Silicone Injection at Room Temperature to be Used in Comparative Anatomy learning

Ejemplares similares