The Protective Effects of Citrus aurantium Flower Extract against 6-Hydroxydopamine-Mediated Cell Damage in Human Neuroblastoma SH-SY5Y Cells

SUMMARY: Parkinson’s disease (PD) is described as a neurological condition, resulting from continuous degeneration of dopaminergic neurons. Currently, most treatments for neurodegenerative diseases are palliative. In traditional Iranian medicine, Citrus aurantium flower extract is used to...

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Autores principales:	Elyasi,Leila, Jahanshahi,Mehrdad, Ghazvini,Hamed, Nikmahzar,Emsehgol
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2018
Materias:	Citrus aurantium Parkinson’s disease 6-Hydroxydopamine Apoptosis Antioxidant activity
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022018000200435
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spelling	oai:scielo:S0717-950220180002004352019-09-16The Protective Effects of Citrus aurantium Flower Extract against 6-Hydroxydopamine-Mediated Cell Damage in Human Neuroblastoma SH-SY5Y CellsElyasi,LeilaJahanshahi,MehrdadGhazvini,HamedNikmahzar,Emsehgol Citrus aurantium Parkinson’s disease 6-Hydroxydopamine Apoptosis Antioxidant activity SUMMARY: Parkinson’s disease (PD) is described as a neurological condition, resulting from continuous degeneration of dopaminergic neurons. Currently, most treatments for neurodegenerative diseases are palliative. In traditional Iranian medicine, Citrus aurantium flower extract is used to treat some neural diseases, such as sleep disorders and anxiety. The tendency towards the use of medicinal herbs for the treatment of diseases (eg, seizure) is growing. Accordingly, we evaluated the antioxidant effects of C. aurantium flowers and analyzed their protective effects against 6-hydroxydopamine (6-OHDA)-mediated oxidative stress. In this study, 150 mM of 6-OHDA was used to induce cellular damage. Also, MTT assay was performed to analyze cellular viability. Fluorescence spectrophotometry was performed to measure the intracellular reactive oxygen species (ROS) and calcium levels. Based on the findings, 6-OHDA could reduce cell viability. We also analyzed the effects of C. aurantium against neurotoxicity. The intracellular levels of ROS and calcium greatly improved in cells exposed to 6-OHDA. SH-SY5Y cell incubation with C. aurantium (400 and 600 mg/mL) induced protective effects and decreased the biochemical markers of cell apoptosis. According to the findings, C. aurantium showed protective effects against neurotoxicity, caused by 6-OHDA; these protective properties were accompanied by antiapoptotic features. According to the findings, it seems that hydromethanolic C. aurantium extract can be used to prevent seizures.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.36 n.2 20182018-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022018000200435en10.4067/S0717-95022018000200435
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Citrus aurantium Parkinson’s disease 6-Hydroxydopamine Apoptosis Antioxidant activity
spellingShingle	Citrus aurantium Parkinson’s disease 6-Hydroxydopamine Apoptosis Antioxidant activity Elyasi,Leila Jahanshahi,Mehrdad Ghazvini,Hamed Nikmahzar,Emsehgol The Protective Effects of Citrus aurantium Flower Extract against 6-Hydroxydopamine-Mediated Cell Damage in Human Neuroblastoma SH-SY5Y Cells
description	SUMMARY: Parkinson’s disease (PD) is described as a neurological condition, resulting from continuous degeneration of dopaminergic neurons. Currently, most treatments for neurodegenerative diseases are palliative. In traditional Iranian medicine, Citrus aurantium flower extract is used to treat some neural diseases, such as sleep disorders and anxiety. The tendency towards the use of medicinal herbs for the treatment of diseases (eg, seizure) is growing. Accordingly, we evaluated the antioxidant effects of C. aurantium flowers and analyzed their protective effects against 6-hydroxydopamine (6-OHDA)-mediated oxidative stress. In this study, 150 mM of 6-OHDA was used to induce cellular damage. Also, MTT assay was performed to analyze cellular viability. Fluorescence spectrophotometry was performed to measure the intracellular reactive oxygen species (ROS) and calcium levels. Based on the findings, 6-OHDA could reduce cell viability. We also analyzed the effects of C. aurantium against neurotoxicity. The intracellular levels of ROS and calcium greatly improved in cells exposed to 6-OHDA. SH-SY5Y cell incubation with C. aurantium (400 and 600 mg/mL) induced protective effects and decreased the biochemical markers of cell apoptosis. According to the findings, C. aurantium showed protective effects against neurotoxicity, caused by 6-OHDA; these protective properties were accompanied by antiapoptotic features. According to the findings, it seems that hydromethanolic C. aurantium extract can be used to prevent seizures.
author	Elyasi,Leila Jahanshahi,Mehrdad Ghazvini,Hamed Nikmahzar,Emsehgol
author_facet	Elyasi,Leila Jahanshahi,Mehrdad Ghazvini,Hamed Nikmahzar,Emsehgol
author_sort	Elyasi,Leila
title	The Protective Effects of Citrus aurantium Flower Extract against 6-Hydroxydopamine-Mediated Cell Damage in Human Neuroblastoma SH-SY5Y Cells
title_short	The Protective Effects of Citrus aurantium Flower Extract against 6-Hydroxydopamine-Mediated Cell Damage in Human Neuroblastoma SH-SY5Y Cells
title_full	The Protective Effects of Citrus aurantium Flower Extract against 6-Hydroxydopamine-Mediated Cell Damage in Human Neuroblastoma SH-SY5Y Cells
title_fullStr	The Protective Effects of Citrus aurantium Flower Extract against 6-Hydroxydopamine-Mediated Cell Damage in Human Neuroblastoma SH-SY5Y Cells
title_full_unstemmed	The Protective Effects of Citrus aurantium Flower Extract against 6-Hydroxydopamine-Mediated Cell Damage in Human Neuroblastoma SH-SY5Y Cells
title_sort	protective effects of citrus aurantium flower extract against 6-hydroxydopamine-mediated cell damage in human neuroblastoma sh-sy5y cells
publisher	Sociedad Chilena de Anatomía
publishDate	2018
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022018000200435
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The Protective Effects of Citrus aurantium Flower Extract against 6-Hydroxydopamine-Mediated Cell Damage in Human Neuroblastoma SH-SY5Y Cells

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