Antioxidant Capacity of Momordica charantia Extract and its Protective Effect on Testicular Damage in Valproic Acid-Induced Rats

SUMMARY: Although Momordica charantia (MC) has preventive effects on tissue injuries, antioxidant capacity and protective effect of MC pulp and peel (MCP) on valproic acid (VPA)-testicular damage have never been reported. Fresh MCPs were aqueous extracted and determined for antioxidant capacity and...

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Autores principales:	Maneenin,Chanwit, Burawat,Jaturon, Maneenin,Naowarat, Nualkaew,Somsak, Arun,Supatcharee, Sampannang,Apichakan, Iamsaard,Sitthichai
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2018
Materias:	Momordica charantia Momordicine I Antioxidant capacity Testicular damage Valproic acid
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022018000200447
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spelling	oai:scielo:S0717-950220180002004472019-09-16Antioxidant Capacity of Momordica charantia Extract and its Protective Effect on Testicular Damage in Valproic Acid-Induced RatsManeenin,ChanwitBurawat,JaturonManeenin,NaowaratNualkaew,SomsakArun,SupatchareeSampannang,ApichakanIamsaard,Sitthichai Momordica charantia Momordicine I Antioxidant capacity Testicular damage Valproic acid SUMMARY: Although Momordica charantia (MC) has preventive effects on tissue injuries, antioxidant capacity and protective effect of MC pulp and peel (MCP) on valproic acid (VPA)-testicular damage have never been reported. Fresh MCPs were aqueous extracted and determined for antioxidant capacity and momordicine I level by HPLC. Male rats were divided into 5 groups (control, VPA (500 mg/kgBW), MCP20/40/ or 80 mg/kgBW+VPA). In 30 experimental days, animals were pretreated with different doses of MCPs for 20 days before VPA injection for 10 consecutive days. Sperm concentration, testosterone hormone, and testicular histology of all groups were investigated. Expressions of testicular tyrosine phosphorylated and steroidogenic acute regulatory (StAR) proteins were examined by Western blot. Results showed that MCP contains TPC (39.24±0.65 ug/mg garlic acid), antioxidant capacities (FRAP=33.08±0.21 ug/ mg ascorbic acid equivalent, IC50 of DPPH=389.8±3.20 ug/ml), and momordicine I (404.9 mg/g MCP). Sperm concentration in MCP80+VPA group was increased as compared to VPA group. Testosterone level in MCP treated groups was significantly increased. MCP protected testicular damage and could prevent the decrease of StAR and a 50-kDa phosphorylated protein expression in VPAtreated testis. In conclusion, MCP has antioxidant activities and can prevent male reproductive toxicity in VPA-induced rats.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.36 n.2 20182018-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022018000200447en10.4067/S0717-95022018000200447
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Momordica charantia Momordicine I Antioxidant capacity Testicular damage Valproic acid
spellingShingle	Momordica charantia Momordicine I Antioxidant capacity Testicular damage Valproic acid Maneenin,Chanwit Burawat,Jaturon Maneenin,Naowarat Nualkaew,Somsak Arun,Supatcharee Sampannang,Apichakan Iamsaard,Sitthichai Antioxidant Capacity of Momordica charantia Extract and its Protective Effect on Testicular Damage in Valproic Acid-Induced Rats
description	SUMMARY: Although Momordica charantia (MC) has preventive effects on tissue injuries, antioxidant capacity and protective effect of MC pulp and peel (MCP) on valproic acid (VPA)-testicular damage have never been reported. Fresh MCPs were aqueous extracted and determined for antioxidant capacity and momordicine I level by HPLC. Male rats were divided into 5 groups (control, VPA (500 mg/kgBW), MCP20/40/ or 80 mg/kgBW+VPA). In 30 experimental days, animals were pretreated with different doses of MCPs for 20 days before VPA injection for 10 consecutive days. Sperm concentration, testosterone hormone, and testicular histology of all groups were investigated. Expressions of testicular tyrosine phosphorylated and steroidogenic acute regulatory (StAR) proteins were examined by Western blot. Results showed that MCP contains TPC (39.24±0.65 ug/mg garlic acid), antioxidant capacities (FRAP=33.08±0.21 ug/ mg ascorbic acid equivalent, IC50 of DPPH=389.8±3.20 ug/ml), and momordicine I (404.9 mg/g MCP). Sperm concentration in MCP80+VPA group was increased as compared to VPA group. Testosterone level in MCP treated groups was significantly increased. MCP protected testicular damage and could prevent the decrease of StAR and a 50-kDa phosphorylated protein expression in VPAtreated testis. In conclusion, MCP has antioxidant activities and can prevent male reproductive toxicity in VPA-induced rats.
author	Maneenin,Chanwit Burawat,Jaturon Maneenin,Naowarat Nualkaew,Somsak Arun,Supatcharee Sampannang,Apichakan Iamsaard,Sitthichai
author_facet	Maneenin,Chanwit Burawat,Jaturon Maneenin,Naowarat Nualkaew,Somsak Arun,Supatcharee Sampannang,Apichakan Iamsaard,Sitthichai
author_sort	Maneenin,Chanwit
title	Antioxidant Capacity of Momordica charantia Extract and its Protective Effect on Testicular Damage in Valproic Acid-Induced Rats
title_short	Antioxidant Capacity of Momordica charantia Extract and its Protective Effect on Testicular Damage in Valproic Acid-Induced Rats
title_full	Antioxidant Capacity of Momordica charantia Extract and its Protective Effect on Testicular Damage in Valproic Acid-Induced Rats
title_fullStr	Antioxidant Capacity of Momordica charantia Extract and its Protective Effect on Testicular Damage in Valproic Acid-Induced Rats
title_full_unstemmed	Antioxidant Capacity of Momordica charantia Extract and its Protective Effect on Testicular Damage in Valproic Acid-Induced Rats
title_sort	antioxidant capacity of momordica charantia extract and its protective effect on testicular damage in valproic acid-induced rats
publisher	Sociedad Chilena de Anatomía
publishDate	2018
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022018000200447
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Antioxidant Capacity of Momordica charantia Extract and its Protective Effect on Testicular Damage in Valproic Acid-Induced Rats

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