Low Intensity Training Provokes Adaptations on Muscle Fibrosis of a Muscular Dystrophy Model

SUMMARY Duchenne muscular dystrophy (DMD) is a genetic neuromuscular disorder with progressive clinical signs until death, around the second decade of life. Mdx is the most used animal model to pre-clinical studies of DMD. Parameters of exercise on this muscular disease are still unknown. This resea...

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Autores principales:	Pinto,Priscilla Avelino Ferreira, Machado,Alex Sander Dias, Libório,Lívia Rocha, Santos,Ana Paula, Oliveira,Murilo Xavier, Gaiad,Thaís Peixoto
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2018
Materias:	Duchenne muscular dystrophy Exercise Skeletal muscle Collagen
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022018000200471
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spelling	oai:scielo:S0717-950220180002004712019-09-16Low Intensity Training Provokes Adaptations on Muscle Fibrosis of a Muscular Dystrophy ModelPinto,Priscilla Avelino FerreiraMachado,Alex Sander DiasLibório,Lívia RochaSantos,Ana PaulaOliveira,Murilo XavierGaiad,Thaís Peixoto Duchenne muscular dystrophy Exercise Skeletal muscle Collagen SUMMARY Duchenne muscular dystrophy (DMD) is a genetic neuromuscular disorder with progressive clinical signs until death, around the second decade of life. Mdx is the most used animal model to pre-clinical studies of DMD. Parameters of exercise on this muscular disease are still unknown. This research aimed to investigate if the low intensity treadmill training would exacerbate the markers of muscle injury, fibrosis, and the composition of the extracellular matrix by type I and III collagens of the mdx model. Dystrophic 11-week-old male mice were separated in exercised (mdxE, n=8) and sedentary (mdxC, n=8) groups. Wild-type mice were used as control (WT, n=8). Exercised group underwent a LIT protocol (9 m/min, 30min, 3days/week, 60 days) on a horizontal treadmill. Gastrocnemius muscle was collected at day 60 and processed to morphological and morphometric analyzes. Sedentary mdx animals presented inflammatory infiltrate and necrotic fibers. Histochemical analysis revealed that the perimysium of the mdxC group is organized into thick and clustered collagen fibers, which generates a larger area of intramuscular collagen fibers for these animals. Histomorphometry attested that fraction area of collagen fibers of mdxC group was higher than mdxE group (p=0.04) and mdxE group values similar to WT group (p=1.00). Centrally located nuclei fibers and the variance coefficient (VC) of minimal Feret’s diameter was similar in mdxE and mdxC groups (p=1.00) and both groups presented higher mean values than WT group (p<0.00). Immunohistochemistry revealed the presence of type I collagen mainly in the mdxC group. LIT protocol had not exacerbated muscle injuries resulting from the dystrophindeficiency membrane fragility at the same time that had reduced the intramuscular collagen deposition. LIT had positively influenced these markers of dystrophic muscle injury on gastrocnemius muscle of mdx model.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.36 n.2 20182018-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022018000200471en10.4067/S0717-95022018000200471
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Duchenne muscular dystrophy Exercise Skeletal muscle Collagen
spellingShingle	Duchenne muscular dystrophy Exercise Skeletal muscle Collagen Pinto,Priscilla Avelino Ferreira Machado,Alex Sander Dias Libório,Lívia Rocha Santos,Ana Paula Oliveira,Murilo Xavier Gaiad,Thaís Peixoto Low Intensity Training Provokes Adaptations on Muscle Fibrosis of a Muscular Dystrophy Model
description	SUMMARY Duchenne muscular dystrophy (DMD) is a genetic neuromuscular disorder with progressive clinical signs until death, around the second decade of life. Mdx is the most used animal model to pre-clinical studies of DMD. Parameters of exercise on this muscular disease are still unknown. This research aimed to investigate if the low intensity treadmill training would exacerbate the markers of muscle injury, fibrosis, and the composition of the extracellular matrix by type I and III collagens of the mdx model. Dystrophic 11-week-old male mice were separated in exercised (mdxE, n=8) and sedentary (mdxC, n=8) groups. Wild-type mice were used as control (WT, n=8). Exercised group underwent a LIT protocol (9 m/min, 30min, 3days/week, 60 days) on a horizontal treadmill. Gastrocnemius muscle was collected at day 60 and processed to morphological and morphometric analyzes. Sedentary mdx animals presented inflammatory infiltrate and necrotic fibers. Histochemical analysis revealed that the perimysium of the mdxC group is organized into thick and clustered collagen fibers, which generates a larger area of intramuscular collagen fibers for these animals. Histomorphometry attested that fraction area of collagen fibers of mdxC group was higher than mdxE group (p=0.04) and mdxE group values similar to WT group (p=1.00). Centrally located nuclei fibers and the variance coefficient (VC) of minimal Feret’s diameter was similar in mdxE and mdxC groups (p=1.00) and both groups presented higher mean values than WT group (p<0.00). Immunohistochemistry revealed the presence of type I collagen mainly in the mdxC group. LIT protocol had not exacerbated muscle injuries resulting from the dystrophindeficiency membrane fragility at the same time that had reduced the intramuscular collagen deposition. LIT had positively influenced these markers of dystrophic muscle injury on gastrocnemius muscle of mdx model.
author	Pinto,Priscilla Avelino Ferreira Machado,Alex Sander Dias Libório,Lívia Rocha Santos,Ana Paula Oliveira,Murilo Xavier Gaiad,Thaís Peixoto
author_facet	Pinto,Priscilla Avelino Ferreira Machado,Alex Sander Dias Libório,Lívia Rocha Santos,Ana Paula Oliveira,Murilo Xavier Gaiad,Thaís Peixoto
author_sort	Pinto,Priscilla Avelino Ferreira
title	Low Intensity Training Provokes Adaptations on Muscle Fibrosis of a Muscular Dystrophy Model
title_short	Low Intensity Training Provokes Adaptations on Muscle Fibrosis of a Muscular Dystrophy Model
title_full	Low Intensity Training Provokes Adaptations on Muscle Fibrosis of a Muscular Dystrophy Model
title_fullStr	Low Intensity Training Provokes Adaptations on Muscle Fibrosis of a Muscular Dystrophy Model
title_full_unstemmed	Low Intensity Training Provokes Adaptations on Muscle Fibrosis of a Muscular Dystrophy Model
title_sort	low intensity training provokes adaptations on muscle fibrosis of a muscular dystrophy model
publisher	Sociedad Chilena de Anatomía
publishDate	2018
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022018000200471
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_version_	1718445044837384192

Low Intensity Training Provokes Adaptations on Muscle Fibrosis of a Muscular Dystrophy Model

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