Effects of High-fat Diets on Biochemical Profiles and Morpho- Quantitative Characteristics of C57BL/6 Mice Adrenal Glands

SUMMARY: According to the information provided by the World Health Organization (WHO), overweight and obesity are the fifth leading causes of death in the world. Due to the alarming increase of this disorder in recent years, studies have been carried out to evidence harmful effects on human or anima...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Navarrete,Javiera, Vásquez,Bélgica, Vasconcellos,Adriana, del Sol,Mariano, Olave,Enrique, Sandoval,Cristian
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2018
Materias:	Adrenal gland Morphometry Stereology High fat diet Corticosterone
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022018000200722
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0717-95022018000200722
record_format	dspace
spelling	oai:scielo:S0717-950220180002007222019-09-16Effects of High-fat Diets on Biochemical Profiles and Morpho- Quantitative Characteristics of C57BL/6 Mice Adrenal GlandsNavarrete,JavieraVásquez,BélgicaVasconcellos,Adrianadel Sol,MarianoOlave,EnriqueSandoval,Cristian Adrenal gland Morphometry Stereology High fat diet Corticosterone SUMMARY: According to the information provided by the World Health Organization (WHO), overweight and obesity are the fifth leading causes of death in the world. Due to the alarming increase of this disorder in recent years, studies have been carried out to evidence harmful effects on human or animal tissues. Our objective was to evaluate the morphological and physiological changes of C57BL/6 mice adrenal glands (AGs), associated with high fat diets (HFDs), we used 14 male mice, 5 months old, fed for 14 weeks according to two diets. The standard chow group (SC) was fed AIN-93M and the high fat group (HF) was fed AIN-93M-HF. At the end of the experiment, biometric analysis was performed and euthanasia was performed. Blood was then extracted for biochemical analysis and AGs were processed for mesoscopic, histological, morphometric and stereological studies. We used Student’s t-test (p<0.05) for statistical analysis. SC group showed a lower weight (29.67±1.28 g) with respect to the HF group (38.46±4.68 g, p=0.002). COL-T, HDL-C, TG and CORT analysis revealed differences between SC group and HF (p≤0.001). Focally, in the fasciculate area, there was an increase in the core-cytoplasm ratio and a greater lipid vacuole presence and size. There was a significant reduction (p=0.001) in Vvfas=7.365±3.326 % of the HF group fasciculate area compared to SC group (Vvfas=9.619±4.548 %). Obesity induced by HF diets affects adrenal gland physiology and morphology of mice. Our results suggest that both the percentage of fat as well as the time of administration of the diet, produce a diurnal reduction of corticosterone, which could be due to an increase in the metabolic clearance of this hormone and not to the inhibition of the Hypothalamus-Pituitary-Adrenal (HPA) axis.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.36 n.2 20182018-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022018000200722en10.4067/S0717-95022018000200722
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Adrenal gland Morphometry Stereology High fat diet Corticosterone
spellingShingle	Adrenal gland Morphometry Stereology High fat diet Corticosterone Navarrete,Javiera Vásquez,Bélgica Vasconcellos,Adriana del Sol,Mariano Olave,Enrique Sandoval,Cristian Effects of High-fat Diets on Biochemical Profiles and Morpho- Quantitative Characteristics of C57BL/6 Mice Adrenal Glands
description	SUMMARY: According to the information provided by the World Health Organization (WHO), overweight and obesity are the fifth leading causes of death in the world. Due to the alarming increase of this disorder in recent years, studies have been carried out to evidence harmful effects on human or animal tissues. Our objective was to evaluate the morphological and physiological changes of C57BL/6 mice adrenal glands (AGs), associated with high fat diets (HFDs), we used 14 male mice, 5 months old, fed for 14 weeks according to two diets. The standard chow group (SC) was fed AIN-93M and the high fat group (HF) was fed AIN-93M-HF. At the end of the experiment, biometric analysis was performed and euthanasia was performed. Blood was then extracted for biochemical analysis and AGs were processed for mesoscopic, histological, morphometric and stereological studies. We used Student’s t-test (p<0.05) for statistical analysis. SC group showed a lower weight (29.67±1.28 g) with respect to the HF group (38.46±4.68 g, p=0.002). COL-T, HDL-C, TG and CORT analysis revealed differences between SC group and HF (p≤0.001). Focally, in the fasciculate area, there was an increase in the core-cytoplasm ratio and a greater lipid vacuole presence and size. There was a significant reduction (p=0.001) in Vvfas=7.365±3.326 % of the HF group fasciculate area compared to SC group (Vvfas=9.619±4.548 %). Obesity induced by HF diets affects adrenal gland physiology and morphology of mice. Our results suggest that both the percentage of fat as well as the time of administration of the diet, produce a diurnal reduction of corticosterone, which could be due to an increase in the metabolic clearance of this hormone and not to the inhibition of the Hypothalamus-Pituitary-Adrenal (HPA) axis.
author	Navarrete,Javiera Vásquez,Bélgica Vasconcellos,Adriana del Sol,Mariano Olave,Enrique Sandoval,Cristian
author_facet	Navarrete,Javiera Vásquez,Bélgica Vasconcellos,Adriana del Sol,Mariano Olave,Enrique Sandoval,Cristian
author_sort	Navarrete,Javiera
title	Effects of High-fat Diets on Biochemical Profiles and Morpho- Quantitative Characteristics of C57BL/6 Mice Adrenal Glands
title_short	Effects of High-fat Diets on Biochemical Profiles and Morpho- Quantitative Characteristics of C57BL/6 Mice Adrenal Glands
title_full	Effects of High-fat Diets on Biochemical Profiles and Morpho- Quantitative Characteristics of C57BL/6 Mice Adrenal Glands
title_fullStr	Effects of High-fat Diets on Biochemical Profiles and Morpho- Quantitative Characteristics of C57BL/6 Mice Adrenal Glands
title_full_unstemmed	Effects of High-fat Diets on Biochemical Profiles and Morpho- Quantitative Characteristics of C57BL/6 Mice Adrenal Glands
title_sort	effects of high-fat diets on biochemical profiles and morpho- quantitative characteristics of c57bl/6 mice adrenal glands
publisher	Sociedad Chilena de Anatomía
publishDate	2018
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022018000200722
work_keys_str_mv	AT navarretejaviera effectsofhighfatdietsonbiochemicalprofilesandmorphoquantitativecharacteristicsofc57bl6miceadrenalglands AT vasquezbelgica effectsofhighfatdietsonbiochemicalprofilesandmorphoquantitativecharacteristicsofc57bl6miceadrenalglands AT vasconcellosadriana effectsofhighfatdietsonbiochemicalprofilesandmorphoquantitativecharacteristicsofc57bl6miceadrenalglands AT delsolmariano effectsofhighfatdietsonbiochemicalprofilesandmorphoquantitativecharacteristicsofc57bl6miceadrenalglands AT olaveenrique effectsofhighfatdietsonbiochemicalprofilesandmorphoquantitativecharacteristicsofc57bl6miceadrenalglands AT sandovalcristian effectsofhighfatdietsonbiochemicalprofilesandmorphoquantitativecharacteristicsofc57bl6miceadrenalglands
_version_	1718445051674099712

Effects of High-fat Diets on Biochemical Profiles and Morpho- Quantitative Characteristics of C57BL/6 Mice Adrenal Glands

Ejemplares similares