Methotrexate Changes the Testicular Tyrosine Phosphorylated Protein Expression and Seminal Vesicle Epithelia of Adult Rats

SUMMARY: Methotrexate (MTX) is commonly used as a chemotherapy agent and immune system suppressant but its adverse effect on male reproductive system is still limited. This study aimed to investigate the effect of MTX on structure and functional proteins of testis and seminal vesicle. Adult male rat...

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Autores principales:	Iamsaard,Sitthichai, Welbat,Jariya Umka, Sukhorum,Wannisa, Krutsri,Suchada, Arun,Supatcharee, Sawatpanich,Tarinee
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2018
Materias:	Methotrexate Phosphorylated proteins Testis Seminal vesicle Male rat
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022018000200737
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spelling	oai:scielo:S0717-950220180002007372019-09-16Methotrexate Changes the Testicular Tyrosine Phosphorylated Protein Expression and Seminal Vesicle Epithelia of Adult RatsIamsaard,SitthichaiWelbat,Jariya UmkaSukhorum,WannisaKrutsri,SuchadaArun,SupatchareeSawatpanich,Tarinee Methotrexate Phosphorylated proteins Testis Seminal vesicle Male rat SUMMARY: Methotrexate (MTX) is commonly used as a chemotherapy agent and immune system suppressant but its adverse effect on male reproductive system is still limited. This study aimed to investigate the effect of MTX on structure and functional proteins of testis and seminal vesicle. Adult male rats were divided into control and MTX groups (n =12). In 30 experimental days, the treated animals were injected with MTX (tail i.v., 75 mg/KgBW) at days 8 and 15. Then, the reproductive parameters and histology of both groups were examined. Thickness of seminal seminal vesicle epithelia was analyzed. Also, the expressions of testicular tyrosine phosphorylated proteins and steroidogenic acute regulatory (StAR) protein were investigated. The results showed that MTX could significantly decrease epididymal sperm concentration. In addition, the germ cell degeneration, increased spaces of interstitial tissues, and low epididymal sperm mass density were observed in MTX group. The thickness of seminal vesicle epithelia in MTX group was significantly lower than that of control group. Moreover, the intensity of testicular phosphorylated proteins of 31, 32, 72, and 85 kDas was significantly increased while of 42 and 47 kDas in MTX group was decreased as compared to control. The expression of testicular StAR protein in MTX group was also significantly decreased as compared to the control. In conclusion, MTX affects testicular and seminal tissues and changes testicular functional proteins in adult rats.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.36 n.2 20182018-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022018000200737en10.4067/S0717-95022018000200737
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Methotrexate Phosphorylated proteins Testis Seminal vesicle Male rat
spellingShingle	Methotrexate Phosphorylated proteins Testis Seminal vesicle Male rat Iamsaard,Sitthichai Welbat,Jariya Umka Sukhorum,Wannisa Krutsri,Suchada Arun,Supatcharee Sawatpanich,Tarinee Methotrexate Changes the Testicular Tyrosine Phosphorylated Protein Expression and Seminal Vesicle Epithelia of Adult Rats
description	SUMMARY: Methotrexate (MTX) is commonly used as a chemotherapy agent and immune system suppressant but its adverse effect on male reproductive system is still limited. This study aimed to investigate the effect of MTX on structure and functional proteins of testis and seminal vesicle. Adult male rats were divided into control and MTX groups (n =12). In 30 experimental days, the treated animals were injected with MTX (tail i.v., 75 mg/KgBW) at days 8 and 15. Then, the reproductive parameters and histology of both groups were examined. Thickness of seminal seminal vesicle epithelia was analyzed. Also, the expressions of testicular tyrosine phosphorylated proteins and steroidogenic acute regulatory (StAR) protein were investigated. The results showed that MTX could significantly decrease epididymal sperm concentration. In addition, the germ cell degeneration, increased spaces of interstitial tissues, and low epididymal sperm mass density were observed in MTX group. The thickness of seminal vesicle epithelia in MTX group was significantly lower than that of control group. Moreover, the intensity of testicular phosphorylated proteins of 31, 32, 72, and 85 kDas was significantly increased while of 42 and 47 kDas in MTX group was decreased as compared to control. The expression of testicular StAR protein in MTX group was also significantly decreased as compared to the control. In conclusion, MTX affects testicular and seminal tissues and changes testicular functional proteins in adult rats.
author	Iamsaard,Sitthichai Welbat,Jariya Umka Sukhorum,Wannisa Krutsri,Suchada Arun,Supatcharee Sawatpanich,Tarinee
author_facet	Iamsaard,Sitthichai Welbat,Jariya Umka Sukhorum,Wannisa Krutsri,Suchada Arun,Supatcharee Sawatpanich,Tarinee
author_sort	Iamsaard,Sitthichai
title	Methotrexate Changes the Testicular Tyrosine Phosphorylated Protein Expression and Seminal Vesicle Epithelia of Adult Rats
title_short	Methotrexate Changes the Testicular Tyrosine Phosphorylated Protein Expression and Seminal Vesicle Epithelia of Adult Rats
title_full	Methotrexate Changes the Testicular Tyrosine Phosphorylated Protein Expression and Seminal Vesicle Epithelia of Adult Rats
title_fullStr	Methotrexate Changes the Testicular Tyrosine Phosphorylated Protein Expression and Seminal Vesicle Epithelia of Adult Rats
title_full_unstemmed	Methotrexate Changes the Testicular Tyrosine Phosphorylated Protein Expression and Seminal Vesicle Epithelia of Adult Rats
title_sort	methotrexate changes the testicular tyrosine phosphorylated protein expression and seminal vesicle epithelia of adult rats
publisher	Sociedad Chilena de Anatomía
publishDate	2018
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022018000200737
work_keys_str_mv	AT iamsaardsitthichai methotrexatechangesthetesticulartyrosinephosphorylatedproteinexpressionandseminalvesicleepitheliaofadultrats AT welbatjariyaumka methotrexatechangesthetesticulartyrosinephosphorylatedproteinexpressionandseminalvesicleepitheliaofadultrats AT sukhorumwannisa methotrexatechangesthetesticulartyrosinephosphorylatedproteinexpressionandseminalvesicleepitheliaofadultrats AT krutsrisuchada methotrexatechangesthetesticulartyrosinephosphorylatedproteinexpressionandseminalvesicleepitheliaofadultrats AT arunsupatcharee methotrexatechangesthetesticulartyrosinephosphorylatedproteinexpressionandseminalvesicleepitheliaofadultrats AT sawatpanichtarinee methotrexatechangesthetesticulartyrosinephosphorylatedproteinexpressionandseminalvesicleepitheliaofadultrats
_version_	1718445052045295616

Methotrexate Changes the Testicular Tyrosine Phosphorylated Protein Expression and Seminal Vesicle Epithelia of Adult Rats

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