Use of Zebrafish ( Danio rerio ) Embryos as a Model to Assess Effects of Mercury on Developing Skeletal Muscle: A Morphometric and Immunohistochemical Study

SUMMARY: Exposure to mercury in the environment continues to be a significant worldwide concern, especially for developing embryos and fetuses. While extensive research effort has focused on the effects of mercury on the developing nervous system, much less is known concerning adverse effects of mer...

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Autores principales:	Moussa,Eid A, Farouk,Sameh M, Abbott,Louise C, Hassan,Said
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2018
Materias:	Zebrafish Muscle fiber development Striated muscle Myosin Embryonic Development
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022018000300901
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spelling	oai:scielo:S0717-950220180003009012019-09-16Use of Zebrafish ( Danio rerio ) Embryos as a Model to Assess Effects of Mercury on Developing Skeletal Muscle: A Morphometric and Immunohistochemical StudyMoussa,Eid AFarouk,Sameh MAbbott,Louise CHassan,Said Zebrafish Muscle fiber development Striated muscle Myosin Embryonic Development SUMMARY: Exposure to mercury in the environment continues to be a significant worldwide concern, especially for developing embryos and fetuses. While extensive research effort has focused on the effects of mercury on the developing nervous system, much less is known concerning adverse effects of mercury on other organ systems, including the development of skeletal muscle. We exposed developing zebrafish embryos to a range of concentrations of mercuric chloride (100 to 400 µg/liter or ppb) and compared them to control embryos (0 µg/L mercuric chloride). Embryos were examined at 48 hours post fertilization (hpf) for morphometry and morphological deformities of skeletal muscle fibers in the trunk and tail. Embryos exposed to 400 ppb mercuric chloride showed decreased trunk and tail areas compared to control embryos. A dose-dependent reduction in muscle fiber length was observed, and exposure to all concentrations of mercuric chloride used in this study resulted in decreased muscle fiber immunohistochemical staining with anti-myosin antibodies. Irregular muscle fiber diameters, twisted muscle fibers, and degenerated muscle fibers were observed in sections of embryos stained with eosin at the higher exposure concentrations. Evidence presented in this study suggests that exposure to even low concentrations of mercuric chloride adversely affects skeletal muscle fiber development or muscle fiber integrity, or both.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.36 n.3 20182018-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022018000300901en10.4067/S0717-95022018000300901
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Zebrafish Muscle fiber development Striated muscle Myosin Embryonic Development
spellingShingle	Zebrafish Muscle fiber development Striated muscle Myosin Embryonic Development Moussa,Eid A Farouk,Sameh M Abbott,Louise C Hassan,Said Use of Zebrafish ( Danio rerio ) Embryos as a Model to Assess Effects of Mercury on Developing Skeletal Muscle: A Morphometric and Immunohistochemical Study
description	SUMMARY: Exposure to mercury in the environment continues to be a significant worldwide concern, especially for developing embryos and fetuses. While extensive research effort has focused on the effects of mercury on the developing nervous system, much less is known concerning adverse effects of mercury on other organ systems, including the development of skeletal muscle. We exposed developing zebrafish embryos to a range of concentrations of mercuric chloride (100 to 400 µg/liter or ppb) and compared them to control embryos (0 µg/L mercuric chloride). Embryos were examined at 48 hours post fertilization (hpf) for morphometry and morphological deformities of skeletal muscle fibers in the trunk and tail. Embryos exposed to 400 ppb mercuric chloride showed decreased trunk and tail areas compared to control embryos. A dose-dependent reduction in muscle fiber length was observed, and exposure to all concentrations of mercuric chloride used in this study resulted in decreased muscle fiber immunohistochemical staining with anti-myosin antibodies. Irregular muscle fiber diameters, twisted muscle fibers, and degenerated muscle fibers were observed in sections of embryos stained with eosin at the higher exposure concentrations. Evidence presented in this study suggests that exposure to even low concentrations of mercuric chloride adversely affects skeletal muscle fiber development or muscle fiber integrity, or both.
author	Moussa,Eid A Farouk,Sameh M Abbott,Louise C Hassan,Said
author_facet	Moussa,Eid A Farouk,Sameh M Abbott,Louise C Hassan,Said
author_sort	Moussa,Eid A
title	Use of Zebrafish ( Danio rerio ) Embryos as a Model to Assess Effects of Mercury on Developing Skeletal Muscle: A Morphometric and Immunohistochemical Study
title_short	Use of Zebrafish ( Danio rerio ) Embryos as a Model to Assess Effects of Mercury on Developing Skeletal Muscle: A Morphometric and Immunohistochemical Study
title_full	Use of Zebrafish ( Danio rerio ) Embryos as a Model to Assess Effects of Mercury on Developing Skeletal Muscle: A Morphometric and Immunohistochemical Study
title_fullStr	Use of Zebrafish ( Danio rerio ) Embryos as a Model to Assess Effects of Mercury on Developing Skeletal Muscle: A Morphometric and Immunohistochemical Study
title_full_unstemmed	Use of Zebrafish ( Danio rerio ) Embryos as a Model to Assess Effects of Mercury on Developing Skeletal Muscle: A Morphometric and Immunohistochemical Study
title_sort	use of zebrafish ( danio rerio ) embryos as a model to assess effects of mercury on developing skeletal muscle: a morphometric and immunohistochemical study
publisher	Sociedad Chilena de Anatomía
publishDate	2018
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022018000300901
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Use of Zebrafish ( Danio rerio ) Embryos as a Model to Assess Effects of Mercury on Developing Skeletal Muscle: A Morphometric and Immunohistochemical Study

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