Comparative Study of Metachromatic Staining Methods in Assessing the Exfoliative Cell Types During Oestrous Cycle in Sprague-Dawley Laboratory Rats

SUMMARY: This study was aimed at comparing the commonly used metachromatic stains viz., Papanicolaou stain, WrightGiemsa, Toluidine blue and Methylene blue in the assessment of cell types of the oestrous cycle in rats. Eight female Sprague-Dawley rats aged 8-9 weeks were used for this assessment. Co...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Mohammed,Stephanie, Sundaram,Venkatesan
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2018
Materias:	Papanicolaou Wright-Giemsa Methylene blue Toluidine blue Vaginal cytology
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022018000300962
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0717-95022018000300962
record_format	dspace
spelling	oai:scielo:S0717-950220180003009622019-09-16Comparative Study of Metachromatic Staining Methods in Assessing the Exfoliative Cell Types During Oestrous Cycle in Sprague-Dawley Laboratory RatsMohammed,StephanieSundaram,Venkatesan Papanicolaou Wright-Giemsa Methylene blue Toluidine blue Vaginal cytology SUMMARY: This study was aimed at comparing the commonly used metachromatic stains viz., Papanicolaou stain, WrightGiemsa, Toluidine blue and Methylene blue in the assessment of cell types of the oestrous cycle in rats. Eight female Sprague-Dawley rats aged 8-9 weeks were used for this assessment. Cotton Swabs were gently inserted in the animals vagina to obtain cells from which they were then transferred to glass slides for staining and evaluation under microscopy. The different cell types were compared for their morphological features and clarity of cellular detail under all four stains. The application, advantages and limitations of all stains were then discussed. It was concluded that the selection of the most effective stain in the assessment of vaginal cytology depends on their application to clinical or research which was based on the cellular detail of interest, time, cost and availability of each staining procedure.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.36 n.3 20182018-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022018000300962en10.4067/S0717-95022018000300962
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Papanicolaou Wright-Giemsa Methylene blue Toluidine blue Vaginal cytology
spellingShingle	Papanicolaou Wright-Giemsa Methylene blue Toluidine blue Vaginal cytology Mohammed,Stephanie Sundaram,Venkatesan Comparative Study of Metachromatic Staining Methods in Assessing the Exfoliative Cell Types During Oestrous Cycle in Sprague-Dawley Laboratory Rats
description	SUMMARY: This study was aimed at comparing the commonly used metachromatic stains viz., Papanicolaou stain, WrightGiemsa, Toluidine blue and Methylene blue in the assessment of cell types of the oestrous cycle in rats. Eight female Sprague-Dawley rats aged 8-9 weeks were used for this assessment. Cotton Swabs were gently inserted in the animals vagina to obtain cells from which they were then transferred to glass slides for staining and evaluation under microscopy. The different cell types were compared for their morphological features and clarity of cellular detail under all four stains. The application, advantages and limitations of all stains were then discussed. It was concluded that the selection of the most effective stain in the assessment of vaginal cytology depends on their application to clinical or research which was based on the cellular detail of interest, time, cost and availability of each staining procedure.
author	Mohammed,Stephanie Sundaram,Venkatesan
author_facet	Mohammed,Stephanie Sundaram,Venkatesan
author_sort	Mohammed,Stephanie
title	Comparative Study of Metachromatic Staining Methods in Assessing the Exfoliative Cell Types During Oestrous Cycle in Sprague-Dawley Laboratory Rats
title_short	Comparative Study of Metachromatic Staining Methods in Assessing the Exfoliative Cell Types During Oestrous Cycle in Sprague-Dawley Laboratory Rats
title_full	Comparative Study of Metachromatic Staining Methods in Assessing the Exfoliative Cell Types During Oestrous Cycle in Sprague-Dawley Laboratory Rats
title_fullStr	Comparative Study of Metachromatic Staining Methods in Assessing the Exfoliative Cell Types During Oestrous Cycle in Sprague-Dawley Laboratory Rats
title_full_unstemmed	Comparative Study of Metachromatic Staining Methods in Assessing the Exfoliative Cell Types During Oestrous Cycle in Sprague-Dawley Laboratory Rats
title_sort	comparative study of metachromatic staining methods in assessing the exfoliative cell types during oestrous cycle in sprague-dawley laboratory rats
publisher	Sociedad Chilena de Anatomía
publishDate	2018
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022018000300962
work_keys_str_mv	AT mohammedstephanie comparativestudyofmetachromaticstainingmethodsinassessingtheexfoliativecelltypesduringoestrouscycleinspraguedawleylaboratoryrats AT sundaramvenkatesan comparativestudyofmetachromaticstainingmethodsinassessingtheexfoliativecelltypesduringoestrouscycleinspraguedawleylaboratoryrats
_version_	1718445058331508736

Comparative Study of Metachromatic Staining Methods in Assessing the Exfoliative Cell Types During Oestrous Cycle in Sprague-Dawley Laboratory Rats

Ejemplares similares