Ghrelin Improves the Fine Structure of Atrial Natriuretic Factor (ANF) Granules and Intercalated Disc Junctions in Experimentally-Induced Myocardial Infarction in Rats

SUMMARY: Ghrelin is a novel growth hormone-releasing peptide administered to treat myocardial infarction (MI). However, the underlying mechanism of its protective effects against MI remains unclear. A total of sixty healthy Sprague Dawley male rats were included. The first one is the sham-operated c...

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Autores principales:	Eid,Refaat A, Zaki,Mohamed Samir Ahmed, Alghamdi,Mansour A, Al-Shraim,Mubarak, El-kott,Attalla Farag, Al-Hashem,Fahaid H, Aldera,Hussain, Alkhateeb,Mahmoud A.
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2018
Materias:	Ghrelin Myocardial infarction Atrial natriuretic factor Intercalated disc junctions Rats
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022018000301031
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spelling	oai:scielo:S0717-950220180003010312019-09-16Ghrelin Improves the Fine Structure of Atrial Natriuretic Factor (ANF) Granules and Intercalated Disc Junctions in Experimentally-Induced Myocardial Infarction in RatsEid,Refaat AZaki,Mohamed Samir AhmedAlghamdi,Mansour AAl-Shraim,MubarakEl-kott,Attalla FaragAl-Hashem,Fahaid HAldera,HussainAlkhateeb,Mahmoud A. Ghrelin Myocardial infarction Atrial natriuretic factor Intercalated disc junctions Rats SUMMARY: Ghrelin is a novel growth hormone-releasing peptide administered to treat myocardial infarction (MI). However, the underlying mechanism of its protective effects against MI remains unclear. A total of sixty healthy Sprague Dawley male rats were included. The first one is the sham-operated control group were the rats that underwent the same surgical used to induce MI but without tying the left anterior descending coronary artery (LAD) and received normal saline (0.5 ml) as vehicle; the second MI model group were rats with LAD ligation and received normal saline (0. 5 ml) and the third one is MI+ghrelin group were rats that were exposed to surgery to induce MI but received ghrelin (100 µ/kg, orally, 2x/day). At the end of the experiment after 21 days post-MI, rats were sacrificed and processed for ultrastructural demonstration. Our experiment showed that ghrelin inhibited cardiomyocyte apoptosis. Concomitant administration of ghrelin with MI treated rats of this study appeared to show a considerable protection of the atrial tissues. This study revealed that the sarcoplasm was occupied by normal myofibrils with clear striations and others appeared with minor disruption. Normal distribution of atrionatriuretic factor (ANF) granules and well preserved mitochondrial integrity (preserved cristae, normal size and shape), nucleus chromatin arrangement and striated pattern of clear bands (Z and H) compared to the MI group. Intact intercalated disc with clear identification of fully formed fascia adherence and desmosomes with a reconstruction of gap junction (nexus) was also noticed. Atrial myocytes after myocardial infarction is often associated with subsequent heart failure, which could lead to a fatal outcome. In a rat model of experimental myocardial infarction, peripheral ghrelin administration attenuated myocyte dysfunction, well-preserved desmosome, adherent and gap junction of the intercalated disc and normally distributed ANF granules.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.36 n.3 20182018-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022018000301031en10.4067/S0717-95022018000301031
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Ghrelin Myocardial infarction Atrial natriuretic factor Intercalated disc junctions Rats
spellingShingle	Ghrelin Myocardial infarction Atrial natriuretic factor Intercalated disc junctions Rats Eid,Refaat A Zaki,Mohamed Samir Ahmed Alghamdi,Mansour A Al-Shraim,Mubarak El-kott,Attalla Farag Al-Hashem,Fahaid H Aldera,Hussain Alkhateeb,Mahmoud A. Ghrelin Improves the Fine Structure of Atrial Natriuretic Factor (ANF) Granules and Intercalated Disc Junctions in Experimentally-Induced Myocardial Infarction in Rats
description	SUMMARY: Ghrelin is a novel growth hormone-releasing peptide administered to treat myocardial infarction (MI). However, the underlying mechanism of its protective effects against MI remains unclear. A total of sixty healthy Sprague Dawley male rats were included. The first one is the sham-operated control group were the rats that underwent the same surgical used to induce MI but without tying the left anterior descending coronary artery (LAD) and received normal saline (0.5 ml) as vehicle; the second MI model group were rats with LAD ligation and received normal saline (0. 5 ml) and the third one is MI+ghrelin group were rats that were exposed to surgery to induce MI but received ghrelin (100 µ/kg, orally, 2x/day). At the end of the experiment after 21 days post-MI, rats were sacrificed and processed for ultrastructural demonstration. Our experiment showed that ghrelin inhibited cardiomyocyte apoptosis. Concomitant administration of ghrelin with MI treated rats of this study appeared to show a considerable protection of the atrial tissues. This study revealed that the sarcoplasm was occupied by normal myofibrils with clear striations and others appeared with minor disruption. Normal distribution of atrionatriuretic factor (ANF) granules and well preserved mitochondrial integrity (preserved cristae, normal size and shape), nucleus chromatin arrangement and striated pattern of clear bands (Z and H) compared to the MI group. Intact intercalated disc with clear identification of fully formed fascia adherence and desmosomes with a reconstruction of gap junction (nexus) was also noticed. Atrial myocytes after myocardial infarction is often associated with subsequent heart failure, which could lead to a fatal outcome. In a rat model of experimental myocardial infarction, peripheral ghrelin administration attenuated myocyte dysfunction, well-preserved desmosome, adherent and gap junction of the intercalated disc and normally distributed ANF granules.
author	Eid,Refaat A Zaki,Mohamed Samir Ahmed Alghamdi,Mansour A Al-Shraim,Mubarak El-kott,Attalla Farag Al-Hashem,Fahaid H Aldera,Hussain Alkhateeb,Mahmoud A.
author_facet	Eid,Refaat A Zaki,Mohamed Samir Ahmed Alghamdi,Mansour A Al-Shraim,Mubarak El-kott,Attalla Farag Al-Hashem,Fahaid H Aldera,Hussain Alkhateeb,Mahmoud A.
author_sort	Eid,Refaat A
title	Ghrelin Improves the Fine Structure of Atrial Natriuretic Factor (ANF) Granules and Intercalated Disc Junctions in Experimentally-Induced Myocardial Infarction in Rats
title_short	Ghrelin Improves the Fine Structure of Atrial Natriuretic Factor (ANF) Granules and Intercalated Disc Junctions in Experimentally-Induced Myocardial Infarction in Rats
title_full	Ghrelin Improves the Fine Structure of Atrial Natriuretic Factor (ANF) Granules and Intercalated Disc Junctions in Experimentally-Induced Myocardial Infarction in Rats
title_fullStr	Ghrelin Improves the Fine Structure of Atrial Natriuretic Factor (ANF) Granules and Intercalated Disc Junctions in Experimentally-Induced Myocardial Infarction in Rats
title_full_unstemmed	Ghrelin Improves the Fine Structure of Atrial Natriuretic Factor (ANF) Granules and Intercalated Disc Junctions in Experimentally-Induced Myocardial Infarction in Rats
title_sort	ghrelin improves the fine structure of atrial natriuretic factor (anf) granules and intercalated disc junctions in experimentally-induced myocardial infarction in rats
publisher	Sociedad Chilena de Anatomía
publishDate	2018
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022018000301031
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Ghrelin Improves the Fine Structure of Atrial Natriuretic Factor (ANF) Granules and Intercalated Disc Junctions in Experimentally-Induced Myocardial Infarction in Rats

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