Expansion and Phenotypic Changes of Mouse Bone Marrow Mesenchymal Cells Cultured with FGF-2 and Facial Nerve-Conditioned Medium
SUMMARY: Mesenchymal cells (MCs) exhibit great regenerative potential due to their intrinsic properties and ability to restore tissue function, either directly through transdifferentiation or indirectly through paracrine effects. This study aimed to evaluate morphometric and phenotypic changes in MC...
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| Lenguaje: | English |
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Sociedad Chilena de Anatomía
2018
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oai:scielo:S0717-950220180003010492019-09-16Expansion and Phenotypic Changes of Mouse Bone Marrow Mesenchymal Cells Cultured with FGF-2 and Facial Nerve-Conditioned MediumLucena,Eudes Euler de Souzade-Morais,Hécio Henrique AraújoAraújo,Dayane Pessoa deCavalcanti,José Rodolfo Lopes de PaivaAzevedo,Eduardo Pereira deQueiroz,Dinalva Brito deBotelho,Marco AntônioRêgo,Amália Cinthia Meneses doFilho,Irami AraújoBarboza,Carlos Augusto GalvãoNascimento Júnior,Expedito Silva doCosta,Miriam Stela Maris de OliveiraCavalcante,Jeferson de SousaGuzen,Fausto Pierdoná Mesenchymal stem cells Facial nerve injury Fibroblast growth factor 2 Conditioned medium Plasticity Trans-differentiation SUMMARY: Mesenchymal cells (MCs) exhibit great regenerative potential due to their intrinsic properties and ability to restore tissue function, either directly through transdifferentiation or indirectly through paracrine effects. This study aimed to evaluate morphometric and phenotypic changes in MCs grown with facial nerve-conditioned medium in the presence or absence of fibroblast growth factor 2 (FGF-2). For quantitative phenotypic analysis, the expression of GFAP, OX-42, MAP-2, β-tubulin III, NeuN, and NF-200 was analyzed by immunocytochemistry. Cells cultured with facial nerve-conditioned medium in the presence of FGF-2 expressed GFAP, OX-42, MAP-2, β-tubulin III, NeuN, and NF-200. On average, the area and perimeter of GFAP-positive cells were higher in the group cultured with facial nerve-conditioned medium compared to the group cultured with conditioned medium and FGF-2 (p=0.0001). This study demonstrated the plasticity of MCs for neuronal and glial lineages and opens up new research perspectives in cell therapy and trans.differentiation.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.36 n.3 20182018-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022018000301049en10.4067/S0717-95022018000301049 |
| institution |
Scielo Chile |
| collection |
Scielo Chile |
| language |
English |
| topic |
Mesenchymal stem cells Facial nerve injury Fibroblast growth factor 2 Conditioned medium Plasticity Trans-differentiation |
| spellingShingle |
Mesenchymal stem cells Facial nerve injury Fibroblast growth factor 2 Conditioned medium Plasticity Trans-differentiation Lucena,Eudes Euler de Souza de-Morais,Hécio Henrique Araújo Araújo,Dayane Pessoa de Cavalcanti,José Rodolfo Lopes de Paiva Azevedo,Eduardo Pereira de Queiroz,Dinalva Brito de Botelho,Marco Antônio Rêgo,Amália Cinthia Meneses do Filho,Irami Araújo Barboza,Carlos Augusto Galvão Nascimento Júnior,Expedito Silva do Costa,Miriam Stela Maris de Oliveira Cavalcante,Jeferson de Sousa Guzen,Fausto Pierdoná Expansion and Phenotypic Changes of Mouse Bone Marrow Mesenchymal Cells Cultured with FGF-2 and Facial Nerve-Conditioned Medium |
| description |
SUMMARY: Mesenchymal cells (MCs) exhibit great regenerative potential due to their intrinsic properties and ability to restore tissue function, either directly through transdifferentiation or indirectly through paracrine effects. This study aimed to evaluate morphometric and phenotypic changes in MCs grown with facial nerve-conditioned medium in the presence or absence of fibroblast growth factor 2 (FGF-2). For quantitative phenotypic analysis, the expression of GFAP, OX-42, MAP-2, β-tubulin III, NeuN, and NF-200 was analyzed by immunocytochemistry. Cells cultured with facial nerve-conditioned medium in the presence of FGF-2 expressed GFAP, OX-42, MAP-2, β-tubulin III, NeuN, and NF-200. On average, the area and perimeter of GFAP-positive cells were higher in the group cultured with facial nerve-conditioned medium compared to the group cultured with conditioned medium and FGF-2 (p=0.0001). This study demonstrated the plasticity of MCs for neuronal and glial lineages and opens up new research perspectives in cell therapy and trans.differentiation. |
| author |
Lucena,Eudes Euler de Souza de-Morais,Hécio Henrique Araújo Araújo,Dayane Pessoa de Cavalcanti,José Rodolfo Lopes de Paiva Azevedo,Eduardo Pereira de Queiroz,Dinalva Brito de Botelho,Marco Antônio Rêgo,Amália Cinthia Meneses do Filho,Irami Araújo Barboza,Carlos Augusto Galvão Nascimento Júnior,Expedito Silva do Costa,Miriam Stela Maris de Oliveira Cavalcante,Jeferson de Sousa Guzen,Fausto Pierdoná |
| author_facet |
Lucena,Eudes Euler de Souza de-Morais,Hécio Henrique Araújo Araújo,Dayane Pessoa de Cavalcanti,José Rodolfo Lopes de Paiva Azevedo,Eduardo Pereira de Queiroz,Dinalva Brito de Botelho,Marco Antônio Rêgo,Amália Cinthia Meneses do Filho,Irami Araújo Barboza,Carlos Augusto Galvão Nascimento Júnior,Expedito Silva do Costa,Miriam Stela Maris de Oliveira Cavalcante,Jeferson de Sousa Guzen,Fausto Pierdoná |
| author_sort |
Lucena,Eudes Euler de Souza |
| title |
Expansion and Phenotypic Changes of Mouse Bone Marrow Mesenchymal Cells Cultured with FGF-2 and Facial Nerve-Conditioned Medium |
| title_short |
Expansion and Phenotypic Changes of Mouse Bone Marrow Mesenchymal Cells Cultured with FGF-2 and Facial Nerve-Conditioned Medium |
| title_full |
Expansion and Phenotypic Changes of Mouse Bone Marrow Mesenchymal Cells Cultured with FGF-2 and Facial Nerve-Conditioned Medium |
| title_fullStr |
Expansion and Phenotypic Changes of Mouse Bone Marrow Mesenchymal Cells Cultured with FGF-2 and Facial Nerve-Conditioned Medium |
| title_full_unstemmed |
Expansion and Phenotypic Changes of Mouse Bone Marrow Mesenchymal Cells Cultured with FGF-2 and Facial Nerve-Conditioned Medium |
| title_sort |
expansion and phenotypic changes of mouse bone marrow mesenchymal cells cultured with fgf-2 and facial nerve-conditioned medium |
| publisher |
Sociedad Chilena de Anatomía |
| publishDate |
2018 |
| url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022018000301049 |
| work_keys_str_mv |
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| _version_ |
1718445060911005696 |