Effect of Wheatgrass on DNA Damage, Oxidative Stress Index and Histological Findings in Diabetic Rats

SUMMARY: This study was aimed to search the effect of wheatgrass on the Total Antioxidan (TAS)-Oxidan Status (TOS) and DNA damage in rat with diabetes. The rats used in the study were randomly divided into 4 groups that each of has 10 rats: Control group; 1 ml single dose phosphate-citrate buffer in...

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Autores principales:	Mis,Leyla, Comba,Bahat, Uslu,Sema, Yeltekin,Asli
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2018
Materias:	Wheat Grass Diabetes DNA damage TAS Beta cell
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022018000401235
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spelling	oai:scielo:S0717-950220180004012352019-09-16Effect of Wheatgrass on DNA Damage, Oxidative Stress Index and Histological Findings in Diabetic RatsMis,LeylaComba,BahatUslu,SemaYeltekin,Asli Wheat Grass Diabetes DNA damage TAS Beta cell SUMMARY: This study was aimed to search the effect of wheatgrass on the Total Antioxidan (TAS)-Oxidan Status (TOS) and DNA damage in rat with diabetes. The rats used in the study were randomly divided into 4 groups that each of has 10 rats: Control group; 1 ml single dose phosphate-citrate buffer injected i.p (pH: 4.5), Diabetes group; 45 mg/kg single dose streptozotocin injected i.p., Wheatgrass group; was given oral wheatgrass (10 ml/kg/day) for 6 weeks, Diabetes +Wheatgrass group; 45 mg/kg single dose streptozotocin injected i.p. and wheatgrass (10 ml/kg/day) was given by oral during 6 weeks. After the process of experiment during 6 weeks, blood sample and pancreas tissue were taken. The analysis were done of blood glucose levels, TAS, TOS levels by colorimetric kits; DNA damage by ELISA kits in serum. The pancreas tissues were examined histopathologically. In the group of Diabetes+Wheatgrass was determined that the levels of glucose levels (p<0.001), TOS (p<0.05) and OSI (p<0.01) statistically decreased and heal histopatolojical compared to diabetes group. In the group of Wheatgrass was determined that the levels of TAS p<0.05 statistically increased from other groups. The statistical significance were not found in the level of serum 8OHdG differences between the groups. The beta cells were seen to increase in the group receiving wheatgrass for therapeutic purposes.As a conclusion, it was determined that wheatgrass strengthened the anti-oxidant defense system and reduced the glucose level in diabetic rats.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.36 n.4 20182018-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022018000401235en10.4067/S0717-95022018000401235
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Wheat Grass Diabetes DNA damage TAS Beta cell
spellingShingle	Wheat Grass Diabetes DNA damage TAS Beta cell Mis,Leyla Comba,Bahat Uslu,Sema Yeltekin,Asli Effect of Wheatgrass on DNA Damage, Oxidative Stress Index and Histological Findings in Diabetic Rats
description	SUMMARY: This study was aimed to search the effect of wheatgrass on the Total Antioxidan (TAS)-Oxidan Status (TOS) and DNA damage in rat with diabetes. The rats used in the study were randomly divided into 4 groups that each of has 10 rats: Control group; 1 ml single dose phosphate-citrate buffer injected i.p (pH: 4.5), Diabetes group; 45 mg/kg single dose streptozotocin injected i.p., Wheatgrass group; was given oral wheatgrass (10 ml/kg/day) for 6 weeks, Diabetes +Wheatgrass group; 45 mg/kg single dose streptozotocin injected i.p. and wheatgrass (10 ml/kg/day) was given by oral during 6 weeks. After the process of experiment during 6 weeks, blood sample and pancreas tissue were taken. The analysis were done of blood glucose levels, TAS, TOS levels by colorimetric kits; DNA damage by ELISA kits in serum. The pancreas tissues were examined histopathologically. In the group of Diabetes+Wheatgrass was determined that the levels of glucose levels (p<0.001), TOS (p<0.05) and OSI (p<0.01) statistically decreased and heal histopatolojical compared to diabetes group. In the group of Wheatgrass was determined that the levels of TAS p<0.05 statistically increased from other groups. The statistical significance were not found in the level of serum 8OHdG differences between the groups. The beta cells were seen to increase in the group receiving wheatgrass for therapeutic purposes.As a conclusion, it was determined that wheatgrass strengthened the anti-oxidant defense system and reduced the glucose level in diabetic rats.
author	Mis,Leyla Comba,Bahat Uslu,Sema Yeltekin,Asli
author_facet	Mis,Leyla Comba,Bahat Uslu,Sema Yeltekin,Asli
author_sort	Mis,Leyla
title	Effect of Wheatgrass on DNA Damage, Oxidative Stress Index and Histological Findings in Diabetic Rats
title_short	Effect of Wheatgrass on DNA Damage, Oxidative Stress Index and Histological Findings in Diabetic Rats
title_full	Effect of Wheatgrass on DNA Damage, Oxidative Stress Index and Histological Findings in Diabetic Rats
title_fullStr	Effect of Wheatgrass on DNA Damage, Oxidative Stress Index and Histological Findings in Diabetic Rats
title_full_unstemmed	Effect of Wheatgrass on DNA Damage, Oxidative Stress Index and Histological Findings in Diabetic Rats
title_sort	effect of wheatgrass on dna damage, oxidative stress index and histological findings in diabetic rats
publisher	Sociedad Chilena de Anatomía
publishDate	2018
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022018000401235
work_keys_str_mv	AT misleyla effectofwheatgrassondnadamageoxidativestressindexandhistologicalfindingsindiabeticrats AT combabahat effectofwheatgrassondnadamageoxidativestressindexandhistologicalfindingsindiabeticrats AT uslusema effectofwheatgrassondnadamageoxidativestressindexandhistologicalfindingsindiabeticrats AT yeltekinasli effectofwheatgrassondnadamageoxidativestressindexandhistologicalfindingsindiabeticrats
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Effect of Wheatgrass on DNA Damage, Oxidative Stress Index and Histological Findings in Diabetic Rats

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