Vitamin E Protects Against Hepatocyte Ultrastructural Damage Induced by High Fat Diet in a Rat Model of Pre-Diabetes

SUMMARY: We sought to investigate the potential protective effect of Vitamin E supplementation against hepatocyte ultrastructural alterations induced by high fat diet (HFD) in a rat model of pre-diabetes. Therefore, rats were either fed with HFD (model group) or a standard laboratory chow (control g...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ellatif,Mohamed Abd, El-Karib,Abbas O, Dallak,Mohammad, Eid,Refaat A, Al-Ani,Rihab, Haidara,Mohamed A
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2018
Materias:	Hepatocyte ultrastructure Pre-diabetes Hepatic steatosis Vitamin E Animal model
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022018000401350
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0717-95022018000401350
record_format	dspace
spelling	oai:scielo:S0717-950220180004013502019-09-16Vitamin E Protects Against Hepatocyte Ultrastructural Damage Induced by High Fat Diet in a Rat Model of Pre-DiabetesEllatif,Mohamed AbdEl-Karib,Abbas ODallak,MohammadEid,Refaat AAl-Ani,RihabHaidara,Mohamed A Hepatocyte ultrastructure Pre-diabetes Hepatic steatosis Vitamin E Animal model SUMMARY: We sought to investigate the potential protective effect of Vitamin E supplementation against hepatocyte ultrastructural alterations induced by high fat diet (HFD) in a rat model of pre-diabetes. Therefore, rats were either fed with HFD (model group) or a standard laboratory chow (control group) for 12 weeks before being sacrificed. The protective group fed on a HFD and started the treatment with vitamin E (100 mg/kg/day, i.p) from day 1 until being sacrificed at week 12. The harvested liver tissues were examined using transmission electron microscopy (TEM) and blood samples were assayed for biomarkers of liver injury and prediabetes. TEM images showed that HFD induced profound pathological changes to the hepatocyte ultrastructure as demonstrated by degenerated hepatocytes with damaged cytoplasm that have mitochondrial swelling, dilation of endoplasmic reticulum, blebbing of plasma membranes, and cytoplasmic accumulations of lipid droplets and vacuoles, which were substantially but not completely protected with vitamin E. In addition, HFD significantly (p<0.05) augmented biomarkers of liver injury and pre-diabetes such as alanine aminotransferase (ALT), aspartate aminotransferase (AST), tumor necrosis factor-alpha (TNF-α), malondialdehyde (MDA), total cholesterol (TC), triglycerides (TG), and low density lipoprotein cholesterol (LDL-C), which were significantly (p<0.05) reduced with vitamin E except TNF-α and TC. Furthermore, none of these biomarkers were reduced to the control level by vitamin E. We conclude that vitamin E is a partial protective agent against HFD-induced liver injury and pre-diabetes.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.36 n.4 20182018-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022018000401350en10.4067/S0717-95022018000401350
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Hepatocyte ultrastructure Pre-diabetes Hepatic steatosis Vitamin E Animal model
spellingShingle	Hepatocyte ultrastructure Pre-diabetes Hepatic steatosis Vitamin E Animal model Ellatif,Mohamed Abd El-Karib,Abbas O Dallak,Mohammad Eid,Refaat A Al-Ani,Rihab Haidara,Mohamed A Vitamin E Protects Against Hepatocyte Ultrastructural Damage Induced by High Fat Diet in a Rat Model of Pre-Diabetes
description	SUMMARY: We sought to investigate the potential protective effect of Vitamin E supplementation against hepatocyte ultrastructural alterations induced by high fat diet (HFD) in a rat model of pre-diabetes. Therefore, rats were either fed with HFD (model group) or a standard laboratory chow (control group) for 12 weeks before being sacrificed. The protective group fed on a HFD and started the treatment with vitamin E (100 mg/kg/day, i.p) from day 1 until being sacrificed at week 12. The harvested liver tissues were examined using transmission electron microscopy (TEM) and blood samples were assayed for biomarkers of liver injury and prediabetes. TEM images showed that HFD induced profound pathological changes to the hepatocyte ultrastructure as demonstrated by degenerated hepatocytes with damaged cytoplasm that have mitochondrial swelling, dilation of endoplasmic reticulum, blebbing of plasma membranes, and cytoplasmic accumulations of lipid droplets and vacuoles, which were substantially but not completely protected with vitamin E. In addition, HFD significantly (p<0.05) augmented biomarkers of liver injury and pre-diabetes such as alanine aminotransferase (ALT), aspartate aminotransferase (AST), tumor necrosis factor-alpha (TNF-α), malondialdehyde (MDA), total cholesterol (TC), triglycerides (TG), and low density lipoprotein cholesterol (LDL-C), which were significantly (p<0.05) reduced with vitamin E except TNF-α and TC. Furthermore, none of these biomarkers were reduced to the control level by vitamin E. We conclude that vitamin E is a partial protective agent against HFD-induced liver injury and pre-diabetes.
author	Ellatif,Mohamed Abd El-Karib,Abbas O Dallak,Mohammad Eid,Refaat A Al-Ani,Rihab Haidara,Mohamed A
author_facet	Ellatif,Mohamed Abd El-Karib,Abbas O Dallak,Mohammad Eid,Refaat A Al-Ani,Rihab Haidara,Mohamed A
author_sort	Ellatif,Mohamed Abd
title	Vitamin E Protects Against Hepatocyte Ultrastructural Damage Induced by High Fat Diet in a Rat Model of Pre-Diabetes
title_short	Vitamin E Protects Against Hepatocyte Ultrastructural Damage Induced by High Fat Diet in a Rat Model of Pre-Diabetes
title_full	Vitamin E Protects Against Hepatocyte Ultrastructural Damage Induced by High Fat Diet in a Rat Model of Pre-Diabetes
title_fullStr	Vitamin E Protects Against Hepatocyte Ultrastructural Damage Induced by High Fat Diet in a Rat Model of Pre-Diabetes
title_full_unstemmed	Vitamin E Protects Against Hepatocyte Ultrastructural Damage Induced by High Fat Diet in a Rat Model of Pre-Diabetes
title_sort	vitamin e protects against hepatocyte ultrastructural damage induced by high fat diet in a rat model of pre-diabetes
publisher	Sociedad Chilena de Anatomía
publishDate	2018
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022018000401350
work_keys_str_mv	AT ellatifmohamedabd vitamineprotectsagainsthepatocyteultrastructuraldamageinducedbyhighfatdietinaratmodelofprediabetes AT elkaribabbaso vitamineprotectsagainsthepatocyteultrastructuraldamageinducedbyhighfatdietinaratmodelofprediabetes AT dallakmohammad vitamineprotectsagainsthepatocyteultrastructuraldamageinducedbyhighfatdietinaratmodelofprediabetes AT eidrefaata vitamineprotectsagainsthepatocyteultrastructuraldamageinducedbyhighfatdietinaratmodelofprediabetes AT alanirihab vitamineprotectsagainsthepatocyteultrastructuraldamageinducedbyhighfatdietinaratmodelofprediabetes AT haidaramohameda vitamineprotectsagainsthepatocyteultrastructuraldamageinducedbyhighfatdietinaratmodelofprediabetes
_version_	1718445072857432064

Vitamin E Protects Against Hepatocyte Ultrastructural Damage Induced by High Fat Diet in a Rat Model of Pre-Diabetes

Ejemplares similares