Morphological Observation of Bone Marrow Mesenchymal Stem Cells under Matrigel Three Dimensional Culture Conditions

SUMMARY: Matrigel is a basement membrane matrix extracted from the EHS mouse tumor containing extracellular matrix protein, its main components are laminin, type IV collagen, nestin, heparin sulfate, growth factor and matrix metalloproteinase.At room temperature, Matrigel polymerized to form a three...

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Autores principales:	Liu,Lu-Lu, Zhang,Bin-Bin, Gao,Quan-Wen, Bing,Li, Sha,Huang, Bin,Yao
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2019
Materias:	Morphology Tissue engineering Bone marrow mesenchymal stem cells(BMSCs) Matrigel
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022019000100054
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spelling	oai:scielo:S0717-950220190001000542019-09-11Morphological Observation of Bone Marrow Mesenchymal Stem Cells under Matrigel Three Dimensional Culture ConditionsLiu,Lu-LuZhang,Bin-BinGao,Quan-WenBing,LiSha,HuangBin,Yao Morphology Tissue engineering Bone marrow mesenchymal stem cells(BMSCs) Matrigel SUMMARY: Matrigel is a basement membrane matrix extracted from the EHS mouse tumor containing extracellular matrix protein, its main components are laminin, type IV collagen, nestin, heparin sulfate, growth factor and matrix metalloproteinase.At room temperature, Matrigel polymerized to form a three dimensional matrix with biological activity. It can simulate the structure, composition, physical properties and functions of the cell basement membrane in vivo, which is beneficial to the culture and differentiation of the cells in vitro, and can be used for the study of cell morphology, biochemical function, migration, infection and gene expression. In this study, Matrigel three-dimensional culture model of bone marrow mesenchymal stem cells(BMSCs) was established, and its morphology, proliferation and survival were observed. BMSCs were isolated and cultured with whole bone marrow adherence method. The Second generation BMSCs with good growth condition were selected and mixed with Matrigel to form cell gel complexes. The morphology and proliferation of mesenchymal stem cells were observed by phase contrast microscope and HE staining,Live/Dead staining was used to evaluate the cell activity.Phase contrast microscopy showed that BMSCs were reticulated in Matrigel and proliferated well, After 7 days, the matrix gel gradually became soft and collapsed, a few cell reticular crosslinking growth was seen at 14 days; HE staining showed that the cytoplasm of the cells was larger on the fourth day and the cells were elongated and cross-linked on the seventh day; Live/dead staining showed that most cells showed green fluorescence with the prolongation of culture time, on the first, 4 and 7 days, the activity of bone marrow mesenchymal stem cells in Matrigel gradually increased, and the percentages were 92.57 %, 95.54 % and 97.37 %, respectively. Matrigel three-dimensional culture system can maintain the morphology, function and proliferation ability of bone marrow mesenchymal stem cells.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.37 n.1 20192019-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022019000100054en10.4067/S0717-95022019000100054
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Morphology Tissue engineering Bone marrow mesenchymal stem cells(BMSCs) Matrigel
spellingShingle	Morphology Tissue engineering Bone marrow mesenchymal stem cells(BMSCs) Matrigel Liu,Lu-Lu Zhang,Bin-Bin Gao,Quan-Wen Bing,Li Sha,Huang Bin,Yao Morphological Observation of Bone Marrow Mesenchymal Stem Cells under Matrigel Three Dimensional Culture Conditions
description	SUMMARY: Matrigel is a basement membrane matrix extracted from the EHS mouse tumor containing extracellular matrix protein, its main components are laminin, type IV collagen, nestin, heparin sulfate, growth factor and matrix metalloproteinase.At room temperature, Matrigel polymerized to form a three dimensional matrix with biological activity. It can simulate the structure, composition, physical properties and functions of the cell basement membrane in vivo, which is beneficial to the culture and differentiation of the cells in vitro, and can be used for the study of cell morphology, biochemical function, migration, infection and gene expression. In this study, Matrigel three-dimensional culture model of bone marrow mesenchymal stem cells(BMSCs) was established, and its morphology, proliferation and survival were observed. BMSCs were isolated and cultured with whole bone marrow adherence method. The Second generation BMSCs with good growth condition were selected and mixed with Matrigel to form cell gel complexes. The morphology and proliferation of mesenchymal stem cells were observed by phase contrast microscope and HE staining,Live/Dead staining was used to evaluate the cell activity.Phase contrast microscopy showed that BMSCs were reticulated in Matrigel and proliferated well, After 7 days, the matrix gel gradually became soft and collapsed, a few cell reticular crosslinking growth was seen at 14 days; HE staining showed that the cytoplasm of the cells was larger on the fourth day and the cells were elongated and cross-linked on the seventh day; Live/dead staining showed that most cells showed green fluorescence with the prolongation of culture time, on the first, 4 and 7 days, the activity of bone marrow mesenchymal stem cells in Matrigel gradually increased, and the percentages were 92.57 %, 95.54 % and 97.37 %, respectively. Matrigel three-dimensional culture system can maintain the morphology, function and proliferation ability of bone marrow mesenchymal stem cells.
author	Liu,Lu-Lu Zhang,Bin-Bin Gao,Quan-Wen Bing,Li Sha,Huang Bin,Yao
author_facet	Liu,Lu-Lu Zhang,Bin-Bin Gao,Quan-Wen Bing,Li Sha,Huang Bin,Yao
author_sort	Liu,Lu-Lu
title	Morphological Observation of Bone Marrow Mesenchymal Stem Cells under Matrigel Three Dimensional Culture Conditions
title_short	Morphological Observation of Bone Marrow Mesenchymal Stem Cells under Matrigel Three Dimensional Culture Conditions
title_full	Morphological Observation of Bone Marrow Mesenchymal Stem Cells under Matrigel Three Dimensional Culture Conditions
title_fullStr	Morphological Observation of Bone Marrow Mesenchymal Stem Cells under Matrigel Three Dimensional Culture Conditions
title_full_unstemmed	Morphological Observation of Bone Marrow Mesenchymal Stem Cells under Matrigel Three Dimensional Culture Conditions
title_sort	morphological observation of bone marrow mesenchymal stem cells under matrigel three dimensional culture conditions
publisher	Sociedad Chilena de Anatomía
publishDate	2019
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022019000100054
work_keys_str_mv	AT liululu morphologicalobservationofbonemarrowmesenchymalstemcellsundermatrigelthreedimensionalcultureconditions AT zhangbinbin morphologicalobservationofbonemarrowmesenchymalstemcellsundermatrigelthreedimensionalcultureconditions AT gaoquanwen morphologicalobservationofbonemarrowmesenchymalstemcellsundermatrigelthreedimensionalcultureconditions AT bingli morphologicalobservationofbonemarrowmesenchymalstemcellsundermatrigelthreedimensionalcultureconditions AT shahuang morphologicalobservationofbonemarrowmesenchymalstemcellsundermatrigelthreedimensionalcultureconditions AT binyao morphologicalobservationofbonemarrowmesenchymalstemcellsundermatrigelthreedimensionalcultureconditions
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Morphological Observation of Bone Marrow Mesenchymal Stem Cells under Matrigel Three Dimensional Culture Conditions

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