The Alterations of Microvasculature, Tyrosine Phosphorylation, and Lipid Peroxidation in Kidney of Rats Treated with Valproic Acid

SUMMARY: Valproic acid (VPA), an antiepileptic drug, has been demonstrated to damage histology and to change tyrosine phosphorylation patterns with increased oxidative stress in perirenal tissues. This study aimed to investigate the effect of VPA on microstructure, tyrosine phosphorylation, and lipi...

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Autores principales:	Maneenin,Chanwit, Lapyuneyong,Natthapol, Tongpan,Saranya, Yannasithinon,Supataechasit, Burawat,Jaturon, Maneenin,Naowarat, Sukhorum,Wannisa, Arun,Supatcharee, Iamsaard,Sitthichai
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2019
Materias:	Valproic acid Vasodilatation Tyrosine phosphorylation Kidney Rats
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022019000100065
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spelling	oai:scielo:S0717-950220190001000652019-09-11The Alterations of Microvasculature, Tyrosine Phosphorylation, and Lipid Peroxidation in Kidney of Rats Treated with Valproic AcidManeenin,ChanwitLapyuneyong,NatthapolTongpan,SaranyaYannasithinon,SupataechasitBurawat,JaturonManeenin,NaowaratSukhorum,WannisaArun,SupatchareeIamsaard,Sitthichai Valproic acid Vasodilatation Tyrosine phosphorylation Kidney Rats SUMMARY: Valproic acid (VPA), an antiepileptic drug, has been demonstrated to damage histology and to change tyrosine phosphorylation patterns with increased oxidative stress in perirenal tissues. This study aimed to investigate the effect of VPA on microstructure, tyrosine phosphorylation, and lipid peroxidation of rat kidney. Adult male rats were divided into control and VPA-treated groups intraperitoneally injected with normal saline and VPA 500 mg/kgBW for 10 consecutive days, respectively (n = 7 each). The blood serum was examined for biochemical levels. The kidney tissues were routinely processed for histological observation. Total proteins from kidney were extracted to assay the malondialdehyde (MDA) levels and phosphorylation expression. The results showed that VPA significantly decreased blood glucose levels while tend to increase urea nitrogen and creatinine. MDA levels in VPA group were significantly higher that of control. Renal cortex of VPA-treated animals revealed vasodilatations. Although the ratio of a renal phosphorylated 72 kDa protein/ beta actin expression seemed to be not different in both groups, VPA significantly decreased the intensity of beta actin. In conclusion, VPA dilates renal microvasculature with increasing of MDA but suppresses the actin expression.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.37 n.1 20192019-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022019000100065en10.4067/S0717-95022019000100065
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Valproic acid Vasodilatation Tyrosine phosphorylation Kidney Rats
spellingShingle	Valproic acid Vasodilatation Tyrosine phosphorylation Kidney Rats Maneenin,Chanwit Lapyuneyong,Natthapol Tongpan,Saranya Yannasithinon,Supataechasit Burawat,Jaturon Maneenin,Naowarat Sukhorum,Wannisa Arun,Supatcharee Iamsaard,Sitthichai The Alterations of Microvasculature, Tyrosine Phosphorylation, and Lipid Peroxidation in Kidney of Rats Treated with Valproic Acid
description	SUMMARY: Valproic acid (VPA), an antiepileptic drug, has been demonstrated to damage histology and to change tyrosine phosphorylation patterns with increased oxidative stress in perirenal tissues. This study aimed to investigate the effect of VPA on microstructure, tyrosine phosphorylation, and lipid peroxidation of rat kidney. Adult male rats were divided into control and VPA-treated groups intraperitoneally injected with normal saline and VPA 500 mg/kgBW for 10 consecutive days, respectively (n = 7 each). The blood serum was examined for biochemical levels. The kidney tissues were routinely processed for histological observation. Total proteins from kidney were extracted to assay the malondialdehyde (MDA) levels and phosphorylation expression. The results showed that VPA significantly decreased blood glucose levels while tend to increase urea nitrogen and creatinine. MDA levels in VPA group were significantly higher that of control. Renal cortex of VPA-treated animals revealed vasodilatations. Although the ratio of a renal phosphorylated 72 kDa protein/ beta actin expression seemed to be not different in both groups, VPA significantly decreased the intensity of beta actin. In conclusion, VPA dilates renal microvasculature with increasing of MDA but suppresses the actin expression.
author	Maneenin,Chanwit Lapyuneyong,Natthapol Tongpan,Saranya Yannasithinon,Supataechasit Burawat,Jaturon Maneenin,Naowarat Sukhorum,Wannisa Arun,Supatcharee Iamsaard,Sitthichai
author_facet	Maneenin,Chanwit Lapyuneyong,Natthapol Tongpan,Saranya Yannasithinon,Supataechasit Burawat,Jaturon Maneenin,Naowarat Sukhorum,Wannisa Arun,Supatcharee Iamsaard,Sitthichai
author_sort	Maneenin,Chanwit
title	The Alterations of Microvasculature, Tyrosine Phosphorylation, and Lipid Peroxidation in Kidney of Rats Treated with Valproic Acid
title_short	The Alterations of Microvasculature, Tyrosine Phosphorylation, and Lipid Peroxidation in Kidney of Rats Treated with Valproic Acid
title_full	The Alterations of Microvasculature, Tyrosine Phosphorylation, and Lipid Peroxidation in Kidney of Rats Treated with Valproic Acid
title_fullStr	The Alterations of Microvasculature, Tyrosine Phosphorylation, and Lipid Peroxidation in Kidney of Rats Treated with Valproic Acid
title_full_unstemmed	The Alterations of Microvasculature, Tyrosine Phosphorylation, and Lipid Peroxidation in Kidney of Rats Treated with Valproic Acid
title_sort	alterations of microvasculature, tyrosine phosphorylation, and lipid peroxidation in kidney of rats treated with valproic acid
publisher	Sociedad Chilena de Anatomía
publishDate	2019
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022019000100065
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The Alterations of Microvasculature, Tyrosine Phosphorylation, and Lipid Peroxidation in Kidney of Rats Treated with Valproic Acid

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