Muscle Strength Training is Better than the Use of Growth Hormone (GH) in Bone Health of Wistar Rats

SUMMARY: The objective of this study was to evaluate the effects of growth hormone (GH) and muscle strength training (ST) on the composition of bone tissue of Wistar rats through Raman spectroscopy. In total, 40 male rats were randomly distributed into four groups: (N = 10) control (C), control with...

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Autores principales:	Caetano,Heliard Rodrigues dos Santos, Castoldi,Robson Chacon, Zanuto,Éverton Alex Carvalho, Ozaki,Guilherme Akio Tamura, Garcia,Thiago Alves, Pereira,João Domingos Augusto dos Santos, Constantino,Carlos José Leopoldo, Bremer-Neto,Hermann, Giometti,Ines Cristina, Camargo,Regina Celi Trindade, Camargo-Filho,José Carlos Silva, Belangero,William Dias
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2019
Materias:	Swimming Calcium carbonate Collagen Phosphate Raman spectroscopy
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022019000100104
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spelling	oai:scielo:S0717-950220190001001042019-09-11Muscle Strength Training is Better than the Use of Growth Hormone (GH) in Bone Health of Wistar RatsCaetano,Heliard Rodrigues dos SantosCastoldi,Robson ChaconZanuto,Éverton Alex CarvalhoOzaki,Guilherme Akio TamuraGarcia,Thiago AlvesPereira,João Domingos Augusto dos SantosConstantino,Carlos José LeopoldoBremer-Neto,HermannGiometti,Ines CristinaCamargo,Regina Celi TrindadeCamargo-Filho,José Carlos SilvaBelangero,William Dias Swimming Calcium carbonate Collagen Phosphate Raman spectroscopy SUMMARY: The objective of this study was to evaluate the effects of growth hormone (GH) and muscle strength training (ST) on the composition of bone tissue of Wistar rats through Raman spectroscopy. In total, 40 male rats were randomly distributed into four groups: (N = 10) control (C), control with the application of GH (GHC), strength training (T), and strength training with the application of GH (GHT). The training consisted of four series of 10 water jumps, performed three times a week, with an overload corresponding to 50 % of body weight and duration of four weeks. GH was applied at a dose of 0.2 IU / kg in each animal three times a week and every other day. After four weeks, the animals were euthanized and the right femurs collected for analysis of the bone structure. Raman spectroscopy (ER) was used to observe the following compounds from their respective bands: Calcium Carbonate-Triglycerides (fatty acids) 1073 cm-1, Collagen type I 509 cm-1, Bone-DNA Phosphate (Protein) 589 cm-1, Phosphate Phospholipids 1078 cm-1. For the statistical analysis, the Shapiro-Wilk and ANOVA One-Way variance analysis normality tests were performed, followed by the Tukey post-test. The results showed an increase in the concentrations of calcium carbonate-triglycerides (fatty acids), type I collagen, bone phosphate-DNA (protein), and phosphate phospholipids in all experimental groups, with or without ST and/or GH , But only the isolated training group differed significantly from the control group (P <0.05). It was concluded that all treatments could promote bone tissue gain, however, only the T group demonstrated a significant difference in the mineral compounds analyzed.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.37 n.1 20192019-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022019000100104en10.4067/S0717-95022019000100104
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Swimming Calcium carbonate Collagen Phosphate Raman spectroscopy
spellingShingle	Swimming Calcium carbonate Collagen Phosphate Raman spectroscopy Caetano,Heliard Rodrigues dos Santos Castoldi,Robson Chacon Zanuto,Éverton Alex Carvalho Ozaki,Guilherme Akio Tamura Garcia,Thiago Alves Pereira,João Domingos Augusto dos Santos Constantino,Carlos José Leopoldo Bremer-Neto,Hermann Giometti,Ines Cristina Camargo,Regina Celi Trindade Camargo-Filho,José Carlos Silva Belangero,William Dias Muscle Strength Training is Better than the Use of Growth Hormone (GH) in Bone Health of Wistar Rats
description	SUMMARY: The objective of this study was to evaluate the effects of growth hormone (GH) and muscle strength training (ST) on the composition of bone tissue of Wistar rats through Raman spectroscopy. In total, 40 male rats were randomly distributed into four groups: (N = 10) control (C), control with the application of GH (GHC), strength training (T), and strength training with the application of GH (GHT). The training consisted of four series of 10 water jumps, performed three times a week, with an overload corresponding to 50 % of body weight and duration of four weeks. GH was applied at a dose of 0.2 IU / kg in each animal three times a week and every other day. After four weeks, the animals were euthanized and the right femurs collected for analysis of the bone structure. Raman spectroscopy (ER) was used to observe the following compounds from their respective bands: Calcium Carbonate-Triglycerides (fatty acids) 1073 cm-1, Collagen type I 509 cm-1, Bone-DNA Phosphate (Protein) 589 cm-1, Phosphate Phospholipids 1078 cm-1. For the statistical analysis, the Shapiro-Wilk and ANOVA One-Way variance analysis normality tests were performed, followed by the Tukey post-test. The results showed an increase in the concentrations of calcium carbonate-triglycerides (fatty acids), type I collagen, bone phosphate-DNA (protein), and phosphate phospholipids in all experimental groups, with or without ST and/or GH , But only the isolated training group differed significantly from the control group (P <0.05). It was concluded that all treatments could promote bone tissue gain, however, only the T group demonstrated a significant difference in the mineral compounds analyzed.
author	Caetano,Heliard Rodrigues dos Santos Castoldi,Robson Chacon Zanuto,Éverton Alex Carvalho Ozaki,Guilherme Akio Tamura Garcia,Thiago Alves Pereira,João Domingos Augusto dos Santos Constantino,Carlos José Leopoldo Bremer-Neto,Hermann Giometti,Ines Cristina Camargo,Regina Celi Trindade Camargo-Filho,José Carlos Silva Belangero,William Dias
author_facet	Caetano,Heliard Rodrigues dos Santos Castoldi,Robson Chacon Zanuto,Éverton Alex Carvalho Ozaki,Guilherme Akio Tamura Garcia,Thiago Alves Pereira,João Domingos Augusto dos Santos Constantino,Carlos José Leopoldo Bremer-Neto,Hermann Giometti,Ines Cristina Camargo,Regina Celi Trindade Camargo-Filho,José Carlos Silva Belangero,William Dias
author_sort	Caetano,Heliard Rodrigues dos Santos
title	Muscle Strength Training is Better than the Use of Growth Hormone (GH) in Bone Health of Wistar Rats
title_short	Muscle Strength Training is Better than the Use of Growth Hormone (GH) in Bone Health of Wistar Rats
title_full	Muscle Strength Training is Better than the Use of Growth Hormone (GH) in Bone Health of Wistar Rats
title_fullStr	Muscle Strength Training is Better than the Use of Growth Hormone (GH) in Bone Health of Wistar Rats
title_full_unstemmed	Muscle Strength Training is Better than the Use of Growth Hormone (GH) in Bone Health of Wistar Rats
title_sort	muscle strength training is better than the use of growth hormone (gh) in bone health of wistar rats
publisher	Sociedad Chilena de Anatomía
publishDate	2019
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022019000100104
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Muscle Strength Training is Better than the Use of Growth Hormone (GH) in Bone Health of Wistar Rats

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