Decellularization of Human Umbilical Arteries
SUMMARY: Arterial obstruction in small diameter (<6 mm) vessels are many times treated with grafts, however autologous aren’t always available and synthetic have a high rate of complications. Decellularization of umbilical arteries may provide a solution, but the ideal method is debatab...
Guardado en:
| Autores principales: | , , , , , , , , |
|---|---|
| Lenguaje: | English |
| Publicado: |
Sociedad Chilena de Anatomía
2019
|
| Materias: | |
| Acceso en línea: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022019000100111 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:scielo:S0717-95022019000100111 |
|---|---|
| record_format |
dspace |
| spelling |
oai:scielo:S0717-950220190001001112019-09-11Decellularization of Human Umbilical ArteriesRodríguez-Rodríguez,Víctor EmanuelQuiroga-Garza,AlejandroRodríguez-Roque,Carlos SaúlLoera-Arias,María de JesúsSoto-Domínguez,AdolfoGuzmán-López,SantosVilchez-Cavazos,José FélixMontes-de-Oca-Luna,RobertoElizondo-Omaña,Rodrigo Enrique Decellularization Umbilical artery Vascular graft Extracellular matrix SUMMARY: Arterial obstruction in small diameter (<6 mm) vessels are many times treated with grafts, however autologous aren’t always available and synthetic have a high rate of complications. Decellularization of umbilical arteries may provide a solution, but the ideal method is debatable. We compare effectiveness between SDS and Triton X-100. Umbilical cords obtained from full term pregnancies with normal development and no evident complications in the newborn, were micro-dissected within 12 h and stored in phosphate buffered saline without freezing. Arteries were then processed for decellularization using 0.1 % and 1 % SDS, and 1 % Triton X100 protocols. Evaluation of cellular and nuclear material, collagen fibers, elastic fibers, and glycosoaminoglycans of the extracellular matrix (ECM) were evaluated as well as morphometric analysis under histological and immunohistochemical techniques. Triton X-100 was ineffective, preserving nuclear remains identified by immunofluorescence, had the most notable damage to elastic fibers, and decrease in collagen. SDS effectively eliminated the nuclei and had a less decrease in elastic fibers and collagen. Laminin was preserved in all groups. No significant differences were identified in luminal diameters; however the middle layer decreased due to decellularization of muscle cells. In conclusion, 0.1 % SDS decellularization was the most effective in eliminating cells and preserving the main components of the ECM.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.37 n.1 20192019-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022019000100111en10.4067/S0717-95022019000100111 |
| institution |
Scielo Chile |
| collection |
Scielo Chile |
| language |
English |
| topic |
Decellularization Umbilical artery Vascular graft Extracellular matrix |
| spellingShingle |
Decellularization Umbilical artery Vascular graft Extracellular matrix Rodríguez-Rodríguez,Víctor Emanuel Quiroga-Garza,Alejandro Rodríguez-Roque,Carlos Saúl Loera-Arias,María de Jesús Soto-Domínguez,Adolfo Guzmán-López,Santos Vilchez-Cavazos,José Félix Montes-de-Oca-Luna,Roberto Elizondo-Omaña,Rodrigo Enrique Decellularization of Human Umbilical Arteries |
| description |
SUMMARY: Arterial obstruction in small diameter (<6 mm) vessels are many times treated with grafts, however autologous aren’t always available and synthetic have a high rate of complications. Decellularization of umbilical arteries may provide a solution, but the ideal method is debatable. We compare effectiveness between SDS and Triton X-100. Umbilical cords obtained from full term pregnancies with normal development and no evident complications in the newborn, were micro-dissected within 12 h and stored in phosphate buffered saline without freezing. Arteries were then processed for decellularization using 0.1 % and 1 % SDS, and 1 % Triton X100 protocols. Evaluation of cellular and nuclear material, collagen fibers, elastic fibers, and glycosoaminoglycans of the extracellular matrix (ECM) were evaluated as well as morphometric analysis under histological and immunohistochemical techniques. Triton X-100 was ineffective, preserving nuclear remains identified by immunofluorescence, had the most notable damage to elastic fibers, and decrease in collagen. SDS effectively eliminated the nuclei and had a less decrease in elastic fibers and collagen. Laminin was preserved in all groups. No significant differences were identified in luminal diameters; however the middle layer decreased due to decellularization of muscle cells. In conclusion, 0.1 % SDS decellularization was the most effective in eliminating cells and preserving the main components of the ECM. |
| author |
Rodríguez-Rodríguez,Víctor Emanuel Quiroga-Garza,Alejandro Rodríguez-Roque,Carlos Saúl Loera-Arias,María de Jesús Soto-Domínguez,Adolfo Guzmán-López,Santos Vilchez-Cavazos,José Félix Montes-de-Oca-Luna,Roberto Elizondo-Omaña,Rodrigo Enrique |
| author_facet |
Rodríguez-Rodríguez,Víctor Emanuel Quiroga-Garza,Alejandro Rodríguez-Roque,Carlos Saúl Loera-Arias,María de Jesús Soto-Domínguez,Adolfo Guzmán-López,Santos Vilchez-Cavazos,José Félix Montes-de-Oca-Luna,Roberto Elizondo-Omaña,Rodrigo Enrique |
| author_sort |
Rodríguez-Rodríguez,Víctor Emanuel |
| title |
Decellularization of Human Umbilical Arteries |
| title_short |
Decellularization of Human Umbilical Arteries |
| title_full |
Decellularization of Human Umbilical Arteries |
| title_fullStr |
Decellularization of Human Umbilical Arteries |
| title_full_unstemmed |
Decellularization of Human Umbilical Arteries |
| title_sort |
decellularization of human umbilical arteries |
| publisher |
Sociedad Chilena de Anatomía |
| publishDate |
2019 |
| url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022019000100111 |
| work_keys_str_mv |
AT rodriguezrodriguezvictoremanuel decellularizationofhumanumbilicalarteries AT quirogagarzaalejandro decellularizationofhumanumbilicalarteries AT rodriguezroquecarlossaul decellularizationofhumanumbilicalarteries AT loeraariasmariadejesus decellularizationofhumanumbilicalarteries AT sotodominguezadolfo decellularizationofhumanumbilicalarteries AT guzmanlopezsantos decellularizationofhumanumbilicalarteries AT vilchezcavazosjosefelix decellularizationofhumanumbilicalarteries AT montesdeocalunaroberto decellularizationofhumanumbilicalarteries AT elizondoomanarodrigoenrique decellularizationofhumanumbilicalarteries |
| _version_ |
1718445085608116224 |