Histological and Immunohistochemical Effects of Neuroectodermal Cells Transplantation After Spinal Cord Injury in Rats

SUMMARY: In spinal cord injury, radical treatment is still a persistent hope for patients and clinicians. Our study aimed to determine the different histological changes in central, cranial and caudal sites of compressed spinal cord as a result of neuroectodermal stem cells (NESCs) transplantation i...

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Autores principales:	Al-Karim,Saleh, Ramadan,Wafaa S, Abdel-Hamid,Ghada A, Al-Qudsi,Fatma
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2019
Materias:	Neural Stem cells Compressed Spinal cord Injury Regeneration Rat
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022019000100212
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spelling	oai:scielo:S0717-950220190001002122019-09-11Histological and Immunohistochemical Effects of Neuroectodermal Cells Transplantation After Spinal Cord Injury in RatsAl-Karim,SalehRamadan,Wafaa SAbdel-Hamid,Ghada AAl-Qudsi,Fatma Neural Stem cells Compressed Spinal cord Injury Regeneration Rat SUMMARY: In spinal cord injury, radical treatment is still a persistent hope for patients and clinicians. Our study aimed to determine the different histological changes in central, cranial and caudal sites of compressed spinal cord as a result of neuroectodermal stem cells (NESCs) transplantation in rats. For extraction of NESCs, future brains were extracted from mice embryos (10-days old) and cultured. Eighty, male rats were divided randomly into control, sham (20 rats each); while 40 rats were subjected to compressed spinal cord injury (CSCI). Seven days after spinal cord injury, rats were subdivided into 2 groups (20 rats each); an untreated and treated with NESCs injected cranial and caudal to the site of the spinal cord injury. Rats were sacrificed 4 weeks after transplantations of NESCs and specimens from the spinal cord at the central, cranial and caudal to site of spinal cord injury were proceeded to be stained with haematoxylin & eosin, osmic acid and Immunohistochemistry of glial fibrillary acidic protein (GFAP). Sections of CSCI revealed areas of hemorrhages, necrosis and cavitation limited by reactive astrocytosis, with upregulation of GFAP expression. Evidence of remyelination and mitigation of histopathological features, reactive astrocytosis in CSCI sections were more pronounced in cranial than in caudal region. NESCs transplantation ameliorated the pathological changes, promoted remyelination.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.37 n.1 20192019-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022019000100212en10.4067/S0717-95022019000100212
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Neural Stem cells Compressed Spinal cord Injury Regeneration Rat
spellingShingle	Neural Stem cells Compressed Spinal cord Injury Regeneration Rat Al-Karim,Saleh Ramadan,Wafaa S Abdel-Hamid,Ghada A Al-Qudsi,Fatma Histological and Immunohistochemical Effects of Neuroectodermal Cells Transplantation After Spinal Cord Injury in Rats
description	SUMMARY: In spinal cord injury, radical treatment is still a persistent hope for patients and clinicians. Our study aimed to determine the different histological changes in central, cranial and caudal sites of compressed spinal cord as a result of neuroectodermal stem cells (NESCs) transplantation in rats. For extraction of NESCs, future brains were extracted from mice embryos (10-days old) and cultured. Eighty, male rats were divided randomly into control, sham (20 rats each); while 40 rats were subjected to compressed spinal cord injury (CSCI). Seven days after spinal cord injury, rats were subdivided into 2 groups (20 rats each); an untreated and treated with NESCs injected cranial and caudal to the site of the spinal cord injury. Rats were sacrificed 4 weeks after transplantations of NESCs and specimens from the spinal cord at the central, cranial and caudal to site of spinal cord injury were proceeded to be stained with haematoxylin & eosin, osmic acid and Immunohistochemistry of glial fibrillary acidic protein (GFAP). Sections of CSCI revealed areas of hemorrhages, necrosis and cavitation limited by reactive astrocytosis, with upregulation of GFAP expression. Evidence of remyelination and mitigation of histopathological features, reactive astrocytosis in CSCI sections were more pronounced in cranial than in caudal region. NESCs transplantation ameliorated the pathological changes, promoted remyelination.
author	Al-Karim,Saleh Ramadan,Wafaa S Abdel-Hamid,Ghada A Al-Qudsi,Fatma
author_facet	Al-Karim,Saleh Ramadan,Wafaa S Abdel-Hamid,Ghada A Al-Qudsi,Fatma
author_sort	Al-Karim,Saleh
title	Histological and Immunohistochemical Effects of Neuroectodermal Cells Transplantation After Spinal Cord Injury in Rats
title_short	Histological and Immunohistochemical Effects of Neuroectodermal Cells Transplantation After Spinal Cord Injury in Rats
title_full	Histological and Immunohistochemical Effects of Neuroectodermal Cells Transplantation After Spinal Cord Injury in Rats
title_fullStr	Histological and Immunohistochemical Effects of Neuroectodermal Cells Transplantation After Spinal Cord Injury in Rats
title_full_unstemmed	Histological and Immunohistochemical Effects of Neuroectodermal Cells Transplantation After Spinal Cord Injury in Rats
title_sort	histological and immunohistochemical effects of neuroectodermal cells transplantation after spinal cord injury in rats
publisher	Sociedad Chilena de Anatomía
publishDate	2019
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022019000100212
work_keys_str_mv	AT alkarimsaleh histologicalandimmunohistochemicaleffectsofneuroectodermalcellstransplantationafterspinalcordinjuryinrats AT ramadanwafaas histologicalandimmunohistochemicaleffectsofneuroectodermalcellstransplantationafterspinalcordinjuryinrats AT abdelhamidghadaa histologicalandimmunohistochemicaleffectsofneuroectodermalcellstransplantationafterspinalcordinjuryinrats AT alqudsifatma histologicalandimmunohistochemicaleffectsofneuroectodermalcellstransplantationafterspinalcordinjuryinrats
_version_	1718445088891207680

Histological and Immunohistochemical Effects of Neuroectodermal Cells Transplantation After Spinal Cord Injury in Rats

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