Does Neuroectodermal Stem Cells Transplantation Restore Neural Regeneration and Locomotor Functions in Compressed Spinal Cord Injury Rat Model?

SUMMARY: The aim of this study was to determine the possible regenerative effect of neuroectodermal stem cells on the ultrastructural, and locomotor function resulting from compressed injury to the spinal cord in a rat model. Forty male rats were divided into control and sham groups (20 rats each)....

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Autores principales:	Al-Karim,Saleh, Ramadan,Wafaa S, Abdel-Hamid,Ghada A, Al-Qudsi,Fatma
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2019
Materias:	Neuroectodermal Stem cells Spinal cord Injury Regeneration Rat
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022019000100349
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spelling	oai:scielo:S0717-950220190001003492019-09-11Does Neuroectodermal Stem Cells Transplantation Restore Neural Regeneration and Locomotor Functions in Compressed Spinal Cord Injury Rat Model?Al-Karim,SalehRamadan,Wafaa SAbdel-Hamid,Ghada AAl-Qudsi,Fatma Neuroectodermal Stem cells Spinal cord Injury Regeneration Rat SUMMARY: The aim of this study was to determine the possible regenerative effect of neuroectodermal stem cells on the ultrastructural, and locomotor function resulting from compressed injury to the spinal cord in a rat model. Forty male rats were divided into control and sham groups (20 rats each). Compressed spinal cord injured (CSCI) were forty rats which subdivided equally into: untreated, treated by neuroectodermal stem cells (NESCs). After four weeks, all rats in different groups were scarified, samples were taken from central, cranial, and caudal to the site of spinal cord injury. Specimens were prepared for light and electron microscopic examination. The number of remyelinated axons in central, cranial and caudal regions to the injured spinal cord after transplantation of NESCs was counted. The open field test assessed the locomotor function. Results revealed that compressed spinal cord injury resulted in loss and degeneration of numerous nerve fibers, myelin splitting and degeneration of mitochondria. Four weeks after transplantation of NESCs regenerated axons were noticed in cranial and central sites, while degenerate axons were noticed caudal to the lesion. Number of remyelinated axons was significantly increased in both central and cranial to the site of spinal cord injury in comparison with caudal region which had the least number of remyelinated axons. Transplantation of NESCs improved significantly the locomotor functional activity In conclusion, neuroectodermal stem cells transplantation ameliorated the histopathological and ultrastructural changes, and improved the functional locomotor activity in CSCI rat.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.37 n.1 20192019-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022019000100349en10.4067/S0717-95022019000100349
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Neuroectodermal Stem cells Spinal cord Injury Regeneration Rat
spellingShingle	Neuroectodermal Stem cells Spinal cord Injury Regeneration Rat Al-Karim,Saleh Ramadan,Wafaa S Abdel-Hamid,Ghada A Al-Qudsi,Fatma Does Neuroectodermal Stem Cells Transplantation Restore Neural Regeneration and Locomotor Functions in Compressed Spinal Cord Injury Rat Model?
description	SUMMARY: The aim of this study was to determine the possible regenerative effect of neuroectodermal stem cells on the ultrastructural, and locomotor function resulting from compressed injury to the spinal cord in a rat model. Forty male rats were divided into control and sham groups (20 rats each). Compressed spinal cord injured (CSCI) were forty rats which subdivided equally into: untreated, treated by neuroectodermal stem cells (NESCs). After four weeks, all rats in different groups were scarified, samples were taken from central, cranial, and caudal to the site of spinal cord injury. Specimens were prepared for light and electron microscopic examination. The number of remyelinated axons in central, cranial and caudal regions to the injured spinal cord after transplantation of NESCs was counted. The open field test assessed the locomotor function. Results revealed that compressed spinal cord injury resulted in loss and degeneration of numerous nerve fibers, myelin splitting and degeneration of mitochondria. Four weeks after transplantation of NESCs regenerated axons were noticed in cranial and central sites, while degenerate axons were noticed caudal to the lesion. Number of remyelinated axons was significantly increased in both central and cranial to the site of spinal cord injury in comparison with caudal region which had the least number of remyelinated axons. Transplantation of NESCs improved significantly the locomotor functional activity In conclusion, neuroectodermal stem cells transplantation ameliorated the histopathological and ultrastructural changes, and improved the functional locomotor activity in CSCI rat.
author	Al-Karim,Saleh Ramadan,Wafaa S Abdel-Hamid,Ghada A Al-Qudsi,Fatma
author_facet	Al-Karim,Saleh Ramadan,Wafaa S Abdel-Hamid,Ghada A Al-Qudsi,Fatma
author_sort	Al-Karim,Saleh
title	Does Neuroectodermal Stem Cells Transplantation Restore Neural Regeneration and Locomotor Functions in Compressed Spinal Cord Injury Rat Model?
title_short	Does Neuroectodermal Stem Cells Transplantation Restore Neural Regeneration and Locomotor Functions in Compressed Spinal Cord Injury Rat Model?
title_full	Does Neuroectodermal Stem Cells Transplantation Restore Neural Regeneration and Locomotor Functions in Compressed Spinal Cord Injury Rat Model?
title_fullStr	Does Neuroectodermal Stem Cells Transplantation Restore Neural Regeneration and Locomotor Functions in Compressed Spinal Cord Injury Rat Model?
title_full_unstemmed	Does Neuroectodermal Stem Cells Transplantation Restore Neural Regeneration and Locomotor Functions in Compressed Spinal Cord Injury Rat Model?
title_sort	does neuroectodermal stem cells transplantation restore neural regeneration and locomotor functions in compressed spinal cord injury rat model?
publisher	Sociedad Chilena de Anatomía
publishDate	2019
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022019000100349
work_keys_str_mv	AT alkarimsaleh doesneuroectodermalstemcellstransplantationrestoreneuralregenerationandlocomotorfunctionsincompressedspinalcordinjuryratmodel AT ramadanwafaas doesneuroectodermalstemcellstransplantationrestoreneuralregenerationandlocomotorfunctionsincompressedspinalcordinjuryratmodel AT abdelhamidghadaa doesneuroectodermalstemcellstransplantationrestoreneuralregenerationandlocomotorfunctionsincompressedspinalcordinjuryratmodel AT alqudsifatma doesneuroectodermalstemcellstransplantationrestoreneuralregenerationandlocomotorfunctionsincompressedspinalcordinjuryratmodel
_version_	1718445092915642368

Does Neuroectodermal Stem Cells Transplantation Restore Neural Regeneration and Locomotor Functions in Compressed Spinal Cord Injury Rat Model?

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