Identification of Bacterial and Fungal Species in Human Cadavers Used in Anatomy Teaching

SUMMARY: Within the framework of undergraduate and postgraduate medical education, cadavers have been used to teach anatomy by dissection or by using prosected specimens. To accomplish this, an appropriated preservation process must guarantee that the cadaver is kept safe for harm, destruction, and...

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Autores principales:	Molina,Claudio, Berrocal,Liliana, Jofré,Matías R, Rosas,Carlos, Rojas-S,Ximena
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2019
Materias:	Bacteria Cadavers Dissection Embalming Medical education
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022019000200473
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spelling	oai:scielo:S0717-950220190002004732019-09-11Identification of Bacterial and Fungal Species in Human Cadavers Used in Anatomy TeachingMolina,ClaudioBerrocal,LilianaJofré,Matías RRosas,CarlosRojas-S,Ximena Bacteria Cadavers Dissection Embalming Medical education SUMMARY: Within the framework of undergraduate and postgraduate medical education, cadavers have been used to teach anatomy by dissection or by using prosected specimens. To accomplish this, an appropriated preservation process must guarantee that the cadaver is kept safe for harm, destruction, and decomposition. Embalming fluid contains fixatives, disinfectants, surfactants, buffers, salt, and water, making the cadaver safe for teaching anatomy. However, it remains unclear if there is any risk of dissemination of microorganisms during anatomy teaching, research, and dissection procedures on fixed cadavers. The purpose of this study is to identify bacterial and fungal species in fixed cadaveric material used in anatomy teaching. Samples of cadavers and anatomical sections were cultured and biochemical tests and molecular identification by polymerase chain reaction (PCR) were performed to identify the microorganisms. The results indicate that fixed cadaveric material has viable bacteria on its surfaces and almost all these correspond to gram-negative bacilli of the Enterobacteriaceae family. In conclusion, fixed cadavers could be a reservoir of bacteria. This study underscores the importance of generating safe manipulation protocols to avoid eventual contamination and disease.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.37 n.2 20192019-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022019000200473en10.4067/S0717-95022019000200473
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Bacteria Cadavers Dissection Embalming Medical education
spellingShingle	Bacteria Cadavers Dissection Embalming Medical education Molina,Claudio Berrocal,Liliana Jofré,Matías R Rosas,Carlos Rojas-S,Ximena Identification of Bacterial and Fungal Species in Human Cadavers Used in Anatomy Teaching
description	SUMMARY: Within the framework of undergraduate and postgraduate medical education, cadavers have been used to teach anatomy by dissection or by using prosected specimens. To accomplish this, an appropriated preservation process must guarantee that the cadaver is kept safe for harm, destruction, and decomposition. Embalming fluid contains fixatives, disinfectants, surfactants, buffers, salt, and water, making the cadaver safe for teaching anatomy. However, it remains unclear if there is any risk of dissemination of microorganisms during anatomy teaching, research, and dissection procedures on fixed cadavers. The purpose of this study is to identify bacterial and fungal species in fixed cadaveric material used in anatomy teaching. Samples of cadavers and anatomical sections were cultured and biochemical tests and molecular identification by polymerase chain reaction (PCR) were performed to identify the microorganisms. The results indicate that fixed cadaveric material has viable bacteria on its surfaces and almost all these correspond to gram-negative bacilli of the Enterobacteriaceae family. In conclusion, fixed cadavers could be a reservoir of bacteria. This study underscores the importance of generating safe manipulation protocols to avoid eventual contamination and disease.
author	Molina,Claudio Berrocal,Liliana Jofré,Matías R Rosas,Carlos Rojas-S,Ximena
author_facet	Molina,Claudio Berrocal,Liliana Jofré,Matías R Rosas,Carlos Rojas-S,Ximena
author_sort	Molina,Claudio
title	Identification of Bacterial and Fungal Species in Human Cadavers Used in Anatomy Teaching
title_short	Identification of Bacterial and Fungal Species in Human Cadavers Used in Anatomy Teaching
title_full	Identification of Bacterial and Fungal Species in Human Cadavers Used in Anatomy Teaching
title_fullStr	Identification of Bacterial and Fungal Species in Human Cadavers Used in Anatomy Teaching
title_full_unstemmed	Identification of Bacterial and Fungal Species in Human Cadavers Used in Anatomy Teaching
title_sort	identification of bacterial and fungal species in human cadavers used in anatomy teaching
publisher	Sociedad Chilena de Anatomía
publishDate	2019
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022019000200473
work_keys_str_mv	AT molinaclaudio identificationofbacterialandfungalspeciesinhumancadaversusedinanatomyteaching AT berrocalliliana identificationofbacterialandfungalspeciesinhumancadaversusedinanatomyteaching AT jofrematiasr identificationofbacterialandfungalspeciesinhumancadaversusedinanatomyteaching AT rosascarlos identificationofbacterialandfungalspeciesinhumancadaversusedinanatomyteaching AT rojassximena identificationofbacterialandfungalspeciesinhumancadaversusedinanatomyteaching
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Identification of Bacterial and Fungal Species in Human Cadavers Used in Anatomy Teaching

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