Different Sample Preparation Methods for Glomerular Morphological Evaluation: To Coat or not to Coat?

SUMMARY: The renal glomerulus is coated by fenestrated endothelial cells and externally covered by specialized epithelial cells, known as podocytes. Scanning electron microscopy becomes an important and effective tool for its studies. Normally, samples destined for scanning microscopy are covered wi...

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Autores principales:	dos-Santos,Priscila Fernandes, de-Souza,Diogo Benchimol, Torres,Eduardo José Lopes, Costa,Waldemar Silva, Sampaio,Francisco José Barcelos, Gregorio,Bianca Martins
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2019
Materias:	Glomerular morphology Renal filtration barrier Scanning electron microscopy
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022019000200477
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spelling	oai:scielo:S0717-950220190002004772019-09-11Different Sample Preparation Methods for Glomerular Morphological Evaluation: To Coat or not to Coat?dos-Santos,Priscila Fernandesde-Souza,Diogo BenchimolTorres,Eduardo José LopesCosta,Waldemar SilvaSampaio,Francisco José BarcelosGregorio,Bianca Martins Glomerular morphology Renal filtration barrier Scanning electron microscopy SUMMARY: The renal glomerulus is coated by fenestrated endothelial cells and externally covered by specialized epithelial cells, known as podocytes. Scanning electron microscopy becomes an important and effective tool for its studies. Normally, samples destined for scanning microscopy are covered with a thin metallic layer. However, this step can be dispensed for some analyzes. We aimed to compare coated and uncoated samples for evaluation of the glomerular morphology of the Wistar rat kidney. Cortical region of the kidney of the 5month-old male Wistar rats were used. The fragments followed the routine procedure for scanning electron microscopy processing. Half of 10 fragments were coated with palladium gold and the remaining were not coated. Auriga Compact FIB - SEM scanning electron microscope was used to observe the samples. Different increases and voltages was evaluated. For the uncoated samples, when using voltages of 2 KV (or higher) a great charging was observed, impairing the use of such voltage. Thus, these samples were always observed under voltage of 0.5 KV. On the other hand, in the coated samples, the use of 2 KV was adequate. Almost as a consequence, in the coated samples, the podocyte structures were better characterized, generating better images. Inversely, in the uncoated samples, it was possible to visualize the desired structures and to detect the morphological characteristics of these. The results showed that it is possible to use kidney samples without previous coating to evaluate the glomerular morphology at the ultrastructural level, serving as a tool in the study of pathologies.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.37 n.2 20192019-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022019000200477en10.4067/S0717-95022019000200477
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Glomerular morphology Renal filtration barrier Scanning electron microscopy
spellingShingle	Glomerular morphology Renal filtration barrier Scanning electron microscopy dos-Santos,Priscila Fernandes de-Souza,Diogo Benchimol Torres,Eduardo José Lopes Costa,Waldemar Silva Sampaio,Francisco José Barcelos Gregorio,Bianca Martins Different Sample Preparation Methods for Glomerular Morphological Evaluation: To Coat or not to Coat?
description	SUMMARY: The renal glomerulus is coated by fenestrated endothelial cells and externally covered by specialized epithelial cells, known as podocytes. Scanning electron microscopy becomes an important and effective tool for its studies. Normally, samples destined for scanning microscopy are covered with a thin metallic layer. However, this step can be dispensed for some analyzes. We aimed to compare coated and uncoated samples for evaluation of the glomerular morphology of the Wistar rat kidney. Cortical region of the kidney of the 5month-old male Wistar rats were used. The fragments followed the routine procedure for scanning electron microscopy processing. Half of 10 fragments were coated with palladium gold and the remaining were not coated. Auriga Compact FIB - SEM scanning electron microscope was used to observe the samples. Different increases and voltages was evaluated. For the uncoated samples, when using voltages of 2 KV (or higher) a great charging was observed, impairing the use of such voltage. Thus, these samples were always observed under voltage of 0.5 KV. On the other hand, in the coated samples, the use of 2 KV was adequate. Almost as a consequence, in the coated samples, the podocyte structures were better characterized, generating better images. Inversely, in the uncoated samples, it was possible to visualize the desired structures and to detect the morphological characteristics of these. The results showed that it is possible to use kidney samples without previous coating to evaluate the glomerular morphology at the ultrastructural level, serving as a tool in the study of pathologies.
author	dos-Santos,Priscila Fernandes de-Souza,Diogo Benchimol Torres,Eduardo José Lopes Costa,Waldemar Silva Sampaio,Francisco José Barcelos Gregorio,Bianca Martins
author_facet	dos-Santos,Priscila Fernandes de-Souza,Diogo Benchimol Torres,Eduardo José Lopes Costa,Waldemar Silva Sampaio,Francisco José Barcelos Gregorio,Bianca Martins
author_sort	dos-Santos,Priscila Fernandes
title	Different Sample Preparation Methods for Glomerular Morphological Evaluation: To Coat or not to Coat?
title_short	Different Sample Preparation Methods for Glomerular Morphological Evaluation: To Coat or not to Coat?
title_full	Different Sample Preparation Methods for Glomerular Morphological Evaluation: To Coat or not to Coat?
title_fullStr	Different Sample Preparation Methods for Glomerular Morphological Evaluation: To Coat or not to Coat?
title_full_unstemmed	Different Sample Preparation Methods for Glomerular Morphological Evaluation: To Coat or not to Coat?
title_sort	different sample preparation methods for glomerular morphological evaluation: to coat or not to coat?
publisher	Sociedad Chilena de Anatomía
publishDate	2019
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022019000200477
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Different Sample Preparation Methods for Glomerular Morphological Evaluation: To Coat or not to Coat?

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