Improved Histoarchitectural Changes with Angiotensin Receptor Blockers in Early Testicular and Cauda Toxicity in Rats

SUMMARY: Reproductive dysfunction is a complication for many diseases and toxins. Its early diagnosis and treatment are immensely important. Here the morphological histoarchitecture changes in early testicular and cauda toxicity before and after treatment with angiotensin receptor blockers were eval...

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Autores principales:	Faddladdeen,Khadija Abduljalil, Murad,Hussam Aly, Ali,Soad Shaker
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2019
Materias:	Caspase-3 Masson Proliferating cell nuclear antigen Angiotensin receptor blockers
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022019000200515
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spelling	oai:scielo:S0717-950220190002005152019-09-11Improved Histoarchitectural Changes with Angiotensin Receptor Blockers in Early Testicular and Cauda Toxicity in RatsFaddladdeen,Khadija AbduljalilMurad,Hussam AlyAli,Soad Shaker Caspase-3 Masson Proliferating cell nuclear antigen Angiotensin receptor blockers SUMMARY: Reproductive dysfunction is a complication for many diseases and toxins. Its early diagnosis and treatment are immensely important. Here the morphological histoarchitecture changes in early testicular and cauda toxicity before and after treatment with angiotensin receptor blockers were evaluated. Low-grade testicular damage was induced using thioacetamide (TAA, 50 mg/kg/day) intraperitoneally for two weeks in rats. The rats were randomly divided into four groups (n = 8) treated daily orally for three weeks as follows: Normal control (distilled water), TAA (positive control), TAA+candesartan (0.2 mg/kg) and TAA+losartan (7.5 mg/kg). Serum testosterone and testicular malondialdehyde and glutathione were measured. The changes in histoarchitecture of testis and cauda epididymis were evaluated by hematoxylin and eosin for general structure, Masson's trichrome for collagen, periodic acid Schiff for basement membrane, and caspase-3 and proliferating cell nuclear antigen (PCNA) for immunohistochemical analysis. The TAA-rats showed decreases of serum testosterone and testicular glutathione, increases in testicular malondialdehyde, degenerative changes and apoptosis in germ cells, thickening of tubular basal lamina and increases in expression of caspase 3, and decreases in expression of PCNA. The ARBs (candesartan and losartan) significantly reversed these changes with non-significant differences in-between. Treatment with ARBs (candesartan and losartan) significantly reversed TAA-induced low-grade testicular and cauda toxicity in rats. This could be potentially useful for early treatment of male patients with occupational toxicant-induced reproductive dysfunction especially if they are using ARBs for other comorbidities.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.37 n.2 20192019-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022019000200515en10.4067/S0717-95022019000200515
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Caspase-3 Masson Proliferating cell nuclear antigen Angiotensin receptor blockers
spellingShingle	Caspase-3 Masson Proliferating cell nuclear antigen Angiotensin receptor blockers Faddladdeen,Khadija Abduljalil Murad,Hussam Aly Ali,Soad Shaker Improved Histoarchitectural Changes with Angiotensin Receptor Blockers in Early Testicular and Cauda Toxicity in Rats
description	SUMMARY: Reproductive dysfunction is a complication for many diseases and toxins. Its early diagnosis and treatment are immensely important. Here the morphological histoarchitecture changes in early testicular and cauda toxicity before and after treatment with angiotensin receptor blockers were evaluated. Low-grade testicular damage was induced using thioacetamide (TAA, 50 mg/kg/day) intraperitoneally for two weeks in rats. The rats were randomly divided into four groups (n = 8) treated daily orally for three weeks as follows: Normal control (distilled water), TAA (positive control), TAA+candesartan (0.2 mg/kg) and TAA+losartan (7.5 mg/kg). Serum testosterone and testicular malondialdehyde and glutathione were measured. The changes in histoarchitecture of testis and cauda epididymis were evaluated by hematoxylin and eosin for general structure, Masson's trichrome for collagen, periodic acid Schiff for basement membrane, and caspase-3 and proliferating cell nuclear antigen (PCNA) for immunohistochemical analysis. The TAA-rats showed decreases of serum testosterone and testicular glutathione, increases in testicular malondialdehyde, degenerative changes and apoptosis in germ cells, thickening of tubular basal lamina and increases in expression of caspase 3, and decreases in expression of PCNA. The ARBs (candesartan and losartan) significantly reversed these changes with non-significant differences in-between. Treatment with ARBs (candesartan and losartan) significantly reversed TAA-induced low-grade testicular and cauda toxicity in rats. This could be potentially useful for early treatment of male patients with occupational toxicant-induced reproductive dysfunction especially if they are using ARBs for other comorbidities.
author	Faddladdeen,Khadija Abduljalil Murad,Hussam Aly Ali,Soad Shaker
author_facet	Faddladdeen,Khadija Abduljalil Murad,Hussam Aly Ali,Soad Shaker
author_sort	Faddladdeen,Khadija Abduljalil
title	Improved Histoarchitectural Changes with Angiotensin Receptor Blockers in Early Testicular and Cauda Toxicity in Rats
title_short	Improved Histoarchitectural Changes with Angiotensin Receptor Blockers in Early Testicular and Cauda Toxicity in Rats
title_full	Improved Histoarchitectural Changes with Angiotensin Receptor Blockers in Early Testicular and Cauda Toxicity in Rats
title_fullStr	Improved Histoarchitectural Changes with Angiotensin Receptor Blockers in Early Testicular and Cauda Toxicity in Rats
title_full_unstemmed	Improved Histoarchitectural Changes with Angiotensin Receptor Blockers in Early Testicular and Cauda Toxicity in Rats
title_sort	improved histoarchitectural changes with angiotensin receptor blockers in early testicular and cauda toxicity in rats
publisher	Sociedad Chilena de Anatomía
publishDate	2019
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022019000200515
work_keys_str_mv	AT faddladdeenkhadijaabduljalil improvedhistoarchitecturalchangeswithangiotensinreceptorblockersinearlytesticularandcaudatoxicityinrats AT muradhussamaly improvedhistoarchitecturalchangeswithangiotensinreceptorblockersinearlytesticularandcaudatoxicityinrats AT alisoadshaker improvedhistoarchitecturalchangeswithangiotensinreceptorblockersinearlytesticularandcaudatoxicityinrats
_version_	1718445097685614592

Improved Histoarchitectural Changes with Angiotensin Receptor Blockers in Early Testicular and Cauda Toxicity in Rats

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