Lopinavir and Curcumin Directly Alters BAX/BCL2 and VEGF165b mRNA Levels to Suppress Human Squamous Cervical Carcinoma Cell Growth

SUMMARY: Following the success of the highly active antiretroviral therapy, the potential of multidrug combination regimen for the management of cancer is intensely researched. The anticancer effects of curcumin on some human cell lines have been documented. Lopinavir is a FDA approved protease inhi...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Adefolaju-Gbenga,Anthony, Mwakikunga,Anthony
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2019
Materias:	Lopinavir Curcumin VEGF165b BAX BCL2 Cervical cancer
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022019000200584
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0717-95022019000200584
record_format	dspace
spelling	oai:scielo:S0717-950220190002005842019-09-11Lopinavir and Curcumin Directly Alters BAX/BCL2 and VEGF165b mRNA Levels to Suppress Human Squamous Cervical Carcinoma Cell GrowthAdefolaju-Gbenga,AnthonyMwakikunga,Anthony Lopinavir Curcumin VEGF165b BAX BCL2 Cervical cancer SUMMARY: Following the success of the highly active antiretroviral therapy, the potential of multidrug combination regimen for the management of cancer is intensely researched. The anticancer effects of curcumin on some human cell lines have been documented. Lopinavir is a FDA approved protease inhibitor with known apoptotic activities. Dysregulated apoptosis is important for the initiation of cancer while angiogenesis is required for cancer growth and development, this study therefore investigated the effects of the combination of lopinavir and curcumin on cell viability, apoptosis and the mRNA expression levels of key apoptotic and angiogenic genes; BAX, BCL2 and VEGF165b in two human cervical cell lines; human squamous cell carcinoma cells - uterine cervix (HCS-2) and transformed normal human cervical cells (NCE16IIA). The two human cervical cell lines were treated with physiologically relevant concentrations of the agents for 120 h following which BAX, BCL2 and VEGF165b mRNA expression were determined by Real Time qPCR. The Acridine Orange staining for the morphological evaluation of apoptotic cells was also performed. The combination of lopinavir and curcumin up-regulated pro-apoptotic BAX and antiangiogenic VEGF165b but down-regulated the mRNA levels of anti-apoptotic BCL2 mRNA in the human squamous cell carcinoma (HCS-2) cells only. The fold changes were statistically significant. Micrographs from Acridine Orange staining showed characteristic evidence of apoptosis in the human squamous cell carcinoma (HCS-2) cells only. The findings reported here suggest that the combination of curcumin and the FDA approved drug-lopinavir modulate the apoptotic and angiogenic pathway towards the inhibition of cervical cancer.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.37 n.2 20192019-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022019000200584en10.4067/S0717-95022019000200584
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Lopinavir Curcumin VEGF165b BAX BCL2 Cervical cancer
spellingShingle	Lopinavir Curcumin VEGF165b BAX BCL2 Cervical cancer Adefolaju-Gbenga,Anthony Mwakikunga,Anthony Lopinavir and Curcumin Directly Alters BAX/BCL2 and VEGF165b mRNA Levels to Suppress Human Squamous Cervical Carcinoma Cell Growth
description	SUMMARY: Following the success of the highly active antiretroviral therapy, the potential of multidrug combination regimen for the management of cancer is intensely researched. The anticancer effects of curcumin on some human cell lines have been documented. Lopinavir is a FDA approved protease inhibitor with known apoptotic activities. Dysregulated apoptosis is important for the initiation of cancer while angiogenesis is required for cancer growth and development, this study therefore investigated the effects of the combination of lopinavir and curcumin on cell viability, apoptosis and the mRNA expression levels of key apoptotic and angiogenic genes; BAX, BCL2 and VEGF165b in two human cervical cell lines; human squamous cell carcinoma cells - uterine cervix (HCS-2) and transformed normal human cervical cells (NCE16IIA). The two human cervical cell lines were treated with physiologically relevant concentrations of the agents for 120 h following which BAX, BCL2 and VEGF165b mRNA expression were determined by Real Time qPCR. The Acridine Orange staining for the morphological evaluation of apoptotic cells was also performed. The combination of lopinavir and curcumin up-regulated pro-apoptotic BAX and antiangiogenic VEGF165b but down-regulated the mRNA levels of anti-apoptotic BCL2 mRNA in the human squamous cell carcinoma (HCS-2) cells only. The fold changes were statistically significant. Micrographs from Acridine Orange staining showed characteristic evidence of apoptosis in the human squamous cell carcinoma (HCS-2) cells only. The findings reported here suggest that the combination of curcumin and the FDA approved drug-lopinavir modulate the apoptotic and angiogenic pathway towards the inhibition of cervical cancer.
author	Adefolaju-Gbenga,Anthony Mwakikunga,Anthony
author_facet	Adefolaju-Gbenga,Anthony Mwakikunga,Anthony
author_sort	Adefolaju-Gbenga,Anthony
title	Lopinavir and Curcumin Directly Alters BAX/BCL2 and VEGF165b mRNA Levels to Suppress Human Squamous Cervical Carcinoma Cell Growth
title_short	Lopinavir and Curcumin Directly Alters BAX/BCL2 and VEGF165b mRNA Levels to Suppress Human Squamous Cervical Carcinoma Cell Growth
title_full	Lopinavir and Curcumin Directly Alters BAX/BCL2 and VEGF165b mRNA Levels to Suppress Human Squamous Cervical Carcinoma Cell Growth
title_fullStr	Lopinavir and Curcumin Directly Alters BAX/BCL2 and VEGF165b mRNA Levels to Suppress Human Squamous Cervical Carcinoma Cell Growth
title_full_unstemmed	Lopinavir and Curcumin Directly Alters BAX/BCL2 and VEGF165b mRNA Levels to Suppress Human Squamous Cervical Carcinoma Cell Growth
title_sort	lopinavir and curcumin directly alters bax/bcl2 and vegf165b mrna levels to suppress human squamous cervical carcinoma cell growth
publisher	Sociedad Chilena de Anatomía
publishDate	2019
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022019000200584
work_keys_str_mv	AT adefolajugbengaanthony lopinavirandcurcumindirectlyaltersbaxbcl2andvegf165bmrnalevelstosuppresshumansquamouscervicalcarcinomacellgrowth AT mwakikungaanthony lopinavirandcurcumindirectlyaltersbaxbcl2andvegf165bmrnalevelstosuppresshumansquamouscervicalcarcinomacellgrowth
_version_	1718445099222827008

Lopinavir and Curcumin Directly Alters BAX/BCL2 and VEGF165b mRNA Levels to Suppress Human Squamous Cervical Carcinoma Cell Growth

Ejemplares similares