Pre-Diabetes Induces Ultrastructural Alterations in the Large Blood Vessel Aorta in Rats

SUMMARY: Excessive consumption of carbohydrate and fat increases the risk of cardiovascular disease. We sought to determine the potential ultrastructural alterations in large blood vessels induced by a high fat and fructose diet (HFD) in a rat model of prediabetes. Rats were either fed with HFD (mod...

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Autores principales:	El-Karib,Abbas O, Dallak,Mohammad, Abd-Ellatif,Mohamed, Eid,Refaat A, Haidara,Mohamed A, Al-Ani,Bahjat
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2019
Materias:	Aorta Ultrastructure Pre-diabetes Vascular injury Rat model
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022019000200647
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spelling	oai:scielo:S0717-950220190002006472019-09-11Pre-Diabetes Induces Ultrastructural Alterations in the Large Blood Vessel Aorta in RatsEl-Karib,Abbas ODallak,MohammadAbd-Ellatif,MohamedEid,Refaat AHaidara,Mohamed AAl-Ani,Bahjat Aorta Ultrastructure Pre-diabetes Vascular injury Rat model SUMMARY: Excessive consumption of carbohydrate and fat increases the risk of cardiovascular disease. We sought to determine the potential ultrastructural alterations in large blood vessels induced by a high fat and fructose diet (HFD) in a rat model of prediabetes. Rats were either fed with HFD (model group) or a standard laboratory chow (control group) for 15 weeks before being sacrificed. The harvested thoracic aorta tissues were examined using transmission electron microscopy (TEM), and blood samples were assayed for biomarkers of pre-diabetes.TEM images showed that HFD induced profound pathological changes to the aortic wall layers, tunica intima and tunica media ultrastructures in the pre-diabetic rats as shown by apoptotic endothelial cells with pyknotic nuclei, damaged basal lamina, deteriorated smooth muscle cells that have irregular plasma membranes, shrunken nucleus with clumped nuclear chromatin, damaged mitochondria and few cytoplasmic lipid droplets and vacuoles. In addition, HFD significantly (p<0.05) decreased adiponectin and increased biomarkers of lipidemia, glycaemia, inflammation, oxidative stress, vascular injury such as soluble intercellular adhesion molecule-1 (sICAM-1), soluble vascular cell adhesion protein 1 (sVCAM-1), endothelin-1 (ET-1), and coagulation and thrombosis such as Von Willebrand factor (vWF), and plasminogen activator inhibitor-1 (PAI-1), compared to normal levels of these parameters in the control group. Thus, we demonstrated that feeding rats with a HFDisable to develop a pre-diabetic animal model that is useful to study the aortic ultrastructural alterations.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.37 n.2 20192019-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022019000200647en10.4067/S0717-95022019000200647
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Aorta Ultrastructure Pre-diabetes Vascular injury Rat model
spellingShingle	Aorta Ultrastructure Pre-diabetes Vascular injury Rat model El-Karib,Abbas O Dallak,Mohammad Abd-Ellatif,Mohamed Eid,Refaat A Haidara,Mohamed A Al-Ani,Bahjat Pre-Diabetes Induces Ultrastructural Alterations in the Large Blood Vessel Aorta in Rats
description	SUMMARY: Excessive consumption of carbohydrate and fat increases the risk of cardiovascular disease. We sought to determine the potential ultrastructural alterations in large blood vessels induced by a high fat and fructose diet (HFD) in a rat model of prediabetes. Rats were either fed with HFD (model group) or a standard laboratory chow (control group) for 15 weeks before being sacrificed. The harvested thoracic aorta tissues were examined using transmission electron microscopy (TEM), and blood samples were assayed for biomarkers of pre-diabetes.TEM images showed that HFD induced profound pathological changes to the aortic wall layers, tunica intima and tunica media ultrastructures in the pre-diabetic rats as shown by apoptotic endothelial cells with pyknotic nuclei, damaged basal lamina, deteriorated smooth muscle cells that have irregular plasma membranes, shrunken nucleus with clumped nuclear chromatin, damaged mitochondria and few cytoplasmic lipid droplets and vacuoles. In addition, HFD significantly (p<0.05) decreased adiponectin and increased biomarkers of lipidemia, glycaemia, inflammation, oxidative stress, vascular injury such as soluble intercellular adhesion molecule-1 (sICAM-1), soluble vascular cell adhesion protein 1 (sVCAM-1), endothelin-1 (ET-1), and coagulation and thrombosis such as Von Willebrand factor (vWF), and plasminogen activator inhibitor-1 (PAI-1), compared to normal levels of these parameters in the control group. Thus, we demonstrated that feeding rats with a HFDisable to develop a pre-diabetic animal model that is useful to study the aortic ultrastructural alterations.
author	El-Karib,Abbas O Dallak,Mohammad Abd-Ellatif,Mohamed Eid,Refaat A Haidara,Mohamed A Al-Ani,Bahjat
author_facet	El-Karib,Abbas O Dallak,Mohammad Abd-Ellatif,Mohamed Eid,Refaat A Haidara,Mohamed A Al-Ani,Bahjat
author_sort	El-Karib,Abbas O
title	Pre-Diabetes Induces Ultrastructural Alterations in the Large Blood Vessel Aorta in Rats
title_short	Pre-Diabetes Induces Ultrastructural Alterations in the Large Blood Vessel Aorta in Rats
title_full	Pre-Diabetes Induces Ultrastructural Alterations in the Large Blood Vessel Aorta in Rats
title_fullStr	Pre-Diabetes Induces Ultrastructural Alterations in the Large Blood Vessel Aorta in Rats
title_full_unstemmed	Pre-Diabetes Induces Ultrastructural Alterations in the Large Blood Vessel Aorta in Rats
title_sort	pre-diabetes induces ultrastructural alterations in the large blood vessel aorta in rats
publisher	Sociedad Chilena de Anatomía
publishDate	2019
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022019000200647
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Pre-Diabetes Induces Ultrastructural Alterations in the Large Blood Vessel Aorta in Rats

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