Proliferation and Differentiation of Mouse Spermatogonial Stem Cells on a Three-Dimensional Surface Composed of PCL/Gel Nanofibers

SUMMARY: Spermatogonial stem cells (SSCs) have self-renewal and differentiation capacity essential for sperm production throughout the male reproductive life. The electrospun polycaprolactone/gelatin (PCL/Gel) nanofibrous scaffold mimics important features of the extracellular matrix (ECM), which ca...

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Autores principales:	Talebi,Ali, Sadighi-Gilani,Mohammad Ali, Koruji,Morteza, Ai,Jafar, Navid,Shadan, Rezaie,Mohammad Jafar, Jabari,Ayob, Ashouri-Movassagh,Sepideh, Khadivi,Farnaz, Salehi,Majid, Hoshino,Yumi, Abbasi,Mehdi
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2019
Materias:	Adult germline stem cell Male infertility Cell differentiation Polycaprolactone Nanofibers
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022019000301132
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spelling	oai:scielo:S0717-950220190003011322019-09-11Proliferation and Differentiation of Mouse Spermatogonial Stem Cells on a Three-Dimensional Surface Composed of PCL/Gel NanofibersTalebi,AliSadighi-Gilani,Mohammad AliKoruji,MortezaAi,JafarNavid,ShadanRezaie,Mohammad JafarJabari,AyobAshouri-Movassagh,SepidehKhadivi,FarnazSalehi,MajidHoshino,YumiAbbasi,Mehdi Adult germline stem cell Male infertility Cell differentiation Polycaprolactone Nanofibers SUMMARY: Spermatogonial stem cells (SSCs) have self-renewal and differentiation capacity essential for sperm production throughout the male reproductive life. The electrospun polycaprolactone/gelatin (PCL/Gel) nanofibrous scaffold mimics important features of the extracellular matrix (ECM), which can provide a promising technique for the proliferation and differentiation of SSCs in vitro. The goal of the present study was to investigate the effects of PCL/Gel nanofibrous scaffold on the propagation and differentiation of neonate mouse SSCs (mSSCs). mSSCs were enzymatically isolated, and the cells were purified by differential plating method and seeded on scaffold. After 2 weeks, viability, colony number and diameter, and expression of specific spermatogonial cell genes were investigated. After mSSCs propagation, the cells were cultivated in a differentiation medium on the scaffold for another 2 weeks, and differentiating cells were analyzed by real-time PCR. The number of mSSC colony (P<0.01) and expression levels of specific spermatogonial genes Plzf and Inga6 (P<0.01) and also differentiation genes c-Kit, Tp1 and Ptm1 (P<0.05) were higher in scaffold group compared with control during the culture period. We conclude that mSSCs can be expanded and can differentiate toward spermatid cells on PCL/Gel nanofibrous scaffold with improved developmental parameters.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.37 n.3 20192019-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022019000301132en10.4067/S0717-95022019000301132
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Adult germline stem cell Male infertility Cell differentiation Polycaprolactone Nanofibers
spellingShingle	Adult germline stem cell Male infertility Cell differentiation Polycaprolactone Nanofibers Talebi,Ali Sadighi-Gilani,Mohammad Ali Koruji,Morteza Ai,Jafar Navid,Shadan Rezaie,Mohammad Jafar Jabari,Ayob Ashouri-Movassagh,Sepideh Khadivi,Farnaz Salehi,Majid Hoshino,Yumi Abbasi,Mehdi Proliferation and Differentiation of Mouse Spermatogonial Stem Cells on a Three-Dimensional Surface Composed of PCL/Gel Nanofibers
description	SUMMARY: Spermatogonial stem cells (SSCs) have self-renewal and differentiation capacity essential for sperm production throughout the male reproductive life. The electrospun polycaprolactone/gelatin (PCL/Gel) nanofibrous scaffold mimics important features of the extracellular matrix (ECM), which can provide a promising technique for the proliferation and differentiation of SSCs in vitro. The goal of the present study was to investigate the effects of PCL/Gel nanofibrous scaffold on the propagation and differentiation of neonate mouse SSCs (mSSCs). mSSCs were enzymatically isolated, and the cells were purified by differential plating method and seeded on scaffold. After 2 weeks, viability, colony number and diameter, and expression of specific spermatogonial cell genes were investigated. After mSSCs propagation, the cells were cultivated in a differentiation medium on the scaffold for another 2 weeks, and differentiating cells were analyzed by real-time PCR. The number of mSSC colony (P<0.01) and expression levels of specific spermatogonial genes Plzf and Inga6 (P<0.01) and also differentiation genes c-Kit, Tp1 and Ptm1 (P<0.05) were higher in scaffold group compared with control during the culture period. We conclude that mSSCs can be expanded and can differentiate toward spermatid cells on PCL/Gel nanofibrous scaffold with improved developmental parameters.
author	Talebi,Ali Sadighi-Gilani,Mohammad Ali Koruji,Morteza Ai,Jafar Navid,Shadan Rezaie,Mohammad Jafar Jabari,Ayob Ashouri-Movassagh,Sepideh Khadivi,Farnaz Salehi,Majid Hoshino,Yumi Abbasi,Mehdi
author_facet	Talebi,Ali Sadighi-Gilani,Mohammad Ali Koruji,Morteza Ai,Jafar Navid,Shadan Rezaie,Mohammad Jafar Jabari,Ayob Ashouri-Movassagh,Sepideh Khadivi,Farnaz Salehi,Majid Hoshino,Yumi Abbasi,Mehdi
author_sort	Talebi,Ali
title	Proliferation and Differentiation of Mouse Spermatogonial Stem Cells on a Three-Dimensional Surface Composed of PCL/Gel Nanofibers
title_short	Proliferation and Differentiation of Mouse Spermatogonial Stem Cells on a Three-Dimensional Surface Composed of PCL/Gel Nanofibers
title_full	Proliferation and Differentiation of Mouse Spermatogonial Stem Cells on a Three-Dimensional Surface Composed of PCL/Gel Nanofibers
title_fullStr	Proliferation and Differentiation of Mouse Spermatogonial Stem Cells on a Three-Dimensional Surface Composed of PCL/Gel Nanofibers
title_full_unstemmed	Proliferation and Differentiation of Mouse Spermatogonial Stem Cells on a Three-Dimensional Surface Composed of PCL/Gel Nanofibers
title_sort	proliferation and differentiation of mouse spermatogonial stem cells on a three-dimensional surface composed of pcl/gel nanofibers
publisher	Sociedad Chilena de Anatomía
publishDate	2019
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022019000301132
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Proliferation and Differentiation of Mouse Spermatogonial Stem Cells on a Three-Dimensional Surface Composed of PCL/Gel Nanofibers

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