Collection and Culture of Human Connective Tissue Cells from Gingival Explant Technique for Oral Tissue Bioengineering

SUMMARY: Cell culture is an important tool in medical, odontological and biological research laboratories, supporting cell therapies and tissue bioengineering strategies. Gingival fibroblasts present structural function, being able to modulate their metabolic capacity, which is reflected in the tiss...

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Autores principales:	Abreu,Fernando Antônio Mauad de, Reis,Igor Daniel Garcia, Silva,Gerluza Aparecida Borges, Jorge,Erika Cristina
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2019
Materias:	Cell culture technique Primary cell culture Connective tissue Cells Cellular biology
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022019000401229
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spelling	oai:scielo:S0717-950220190004012292019-09-11Collection and Culture of Human Connective Tissue Cells from Gingival Explant Technique for Oral Tissue BioengineeringAbreu,Fernando Antônio Mauad deReis,Igor Daniel GarciaSilva,Gerluza Aparecida BorgesJorge,Erika Cristina Cell culture technique Primary cell culture Connective tissue Cells Cellular biology SUMMARY: Cell culture is an important tool in medical, odontological and biological research laboratories, supporting cell therapies and tissue bioengineering strategies. Gingival fibroblasts present structural function, being able to modulate their metabolic capacity, which is reflected in the tissue morphology. The possibility of culturing fibroblasts in vitro, in monolayer or on three-dimensional scaffolds, for subsequent transplants in vivo opens important perspectives for the periodontal surgical clinic. The objective of the present article is to present a method of obtaining and cultivating viable human gingival fibroblasts for in vitro research. Explants derived from periodontal surgical discards were used, grown in 25 cm2 bottles to obtain a primary cell culture. After observing the proliferation and growth of the fibroblasts that interconnected and formed a monolayer network, involving the periphery of the explants, it was possible to remove the explants, to make the passage and the new subcultures were obtained in a ratio of 1:1. After 7 days, the amount of viable cells was analyzed in triplicate, using the Neubauer chamber technique, in cell culture bottles of 25 mm2 (T25) and 75 mm2 (T75). Fibroblasts were described and subclassified morphologically. The results showed a growth pattern in both bottles, but with a larger number in bottles of 75 cm2. Cells with fibroblastic morphology were subclassified into reticular and fusiform, being predominant those with fusiform morphology. In conclusion, culture of explant of human gingival connective tissue is a viable method for obtaining gingival connective tissue cells suitable for laboratory tests in cell culture, aiming at obtaining constructs for gingival tissue engineering.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.37 n.4 20192019-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022019000401229en10.4067/S0717-95022019000401229
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Cell culture technique Primary cell culture Connective tissue Cells Cellular biology
spellingShingle	Cell culture technique Primary cell culture Connective tissue Cells Cellular biology Abreu,Fernando Antônio Mauad de Reis,Igor Daniel Garcia Silva,Gerluza Aparecida Borges Jorge,Erika Cristina Collection and Culture of Human Connective Tissue Cells from Gingival Explant Technique for Oral Tissue Bioengineering
description	SUMMARY: Cell culture is an important tool in medical, odontological and biological research laboratories, supporting cell therapies and tissue bioengineering strategies. Gingival fibroblasts present structural function, being able to modulate their metabolic capacity, which is reflected in the tissue morphology. The possibility of culturing fibroblasts in vitro, in monolayer or on three-dimensional scaffolds, for subsequent transplants in vivo opens important perspectives for the periodontal surgical clinic. The objective of the present article is to present a method of obtaining and cultivating viable human gingival fibroblasts for in vitro research. Explants derived from periodontal surgical discards were used, grown in 25 cm2 bottles to obtain a primary cell culture. After observing the proliferation and growth of the fibroblasts that interconnected and formed a monolayer network, involving the periphery of the explants, it was possible to remove the explants, to make the passage and the new subcultures were obtained in a ratio of 1:1. After 7 days, the amount of viable cells was analyzed in triplicate, using the Neubauer chamber technique, in cell culture bottles of 25 mm2 (T25) and 75 mm2 (T75). Fibroblasts were described and subclassified morphologically. The results showed a growth pattern in both bottles, but with a larger number in bottles of 75 cm2. Cells with fibroblastic morphology were subclassified into reticular and fusiform, being predominant those with fusiform morphology. In conclusion, culture of explant of human gingival connective tissue is a viable method for obtaining gingival connective tissue cells suitable for laboratory tests in cell culture, aiming at obtaining constructs for gingival tissue engineering.
author	Abreu,Fernando Antônio Mauad de Reis,Igor Daniel Garcia Silva,Gerluza Aparecida Borges Jorge,Erika Cristina
author_facet	Abreu,Fernando Antônio Mauad de Reis,Igor Daniel Garcia Silva,Gerluza Aparecida Borges Jorge,Erika Cristina
author_sort	Abreu,Fernando Antônio Mauad de
title	Collection and Culture of Human Connective Tissue Cells from Gingival Explant Technique for Oral Tissue Bioengineering
title_short	Collection and Culture of Human Connective Tissue Cells from Gingival Explant Technique for Oral Tissue Bioengineering
title_full	Collection and Culture of Human Connective Tissue Cells from Gingival Explant Technique for Oral Tissue Bioengineering
title_fullStr	Collection and Culture of Human Connective Tissue Cells from Gingival Explant Technique for Oral Tissue Bioengineering
title_full_unstemmed	Collection and Culture of Human Connective Tissue Cells from Gingival Explant Technique for Oral Tissue Bioengineering
title_sort	collection and culture of human connective tissue cells from gingival explant technique for oral tissue bioengineering
publisher	Sociedad Chilena de Anatomía
publishDate	2019
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022019000401229
work_keys_str_mv	AT abreufernandoantoniomauadde collectionandcultureofhumanconnectivetissuecellsfromgingivalexplanttechniquefororaltissuebioengineering AT reisigordanielgarcia collectionandcultureofhumanconnectivetissuecellsfromgingivalexplanttechniquefororaltissuebioengineering AT silvagerluzaaparecidaborges collectionandcultureofhumanconnectivetissuecellsfromgingivalexplanttechniquefororaltissuebioengineering AT jorgeerikacristina collectionandcultureofhumanconnectivetissuecellsfromgingivalexplanttechniquefororaltissuebioengineering
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Collection and Culture of Human Connective Tissue Cells from Gingival Explant Technique for Oral Tissue Bioengineering

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