Pancreatic Islet Stereology: Estimation of Beta Cells Mass

SUMMARY: Obesity and its comorbidities are becoming epidemic in the Western world. Beta cell mass estimation is an important indicator to track the progression of insulin resistance/type 2 diabetes, particularly in experimental studies, where it can be performed with stereological tools in an unbias...

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Auteurs principaux: Marinho,Thatiany de Souza, Aguila,Marcia Barbosa, Mandarim-de-Lacerda,Carlos Alberto
Langue:English
Publié: Sociedad Chilena de Anatomía 2019
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Accès en ligne:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022019000401331
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Résumé:SUMMARY: Obesity and its comorbidities are becoming epidemic in the Western world. Beta cell mass estimation is an important indicator to track the progression of insulin resistance/type 2 diabetes, particularly in experimental studies, where it can be performed with stereological tools in an unbiased way. In this work, we present a simple protocol that can contribute to doing the practice of estimating the mass of beta cells more frequent and reproducible. As with any quantitative study, the necessary precautions regarding sampling and randomness must be respected.