Morphofunctional and Ultrastructural Evaluation of Hip Joint Development in Human Fetuses

SUMMARY: The normal sequential development of the hip joint (HJ) was considered for the evaluation of the morphological and ultrastructural aspects of the joint cartilage of the proximal femoral head epiphysis in human fetuses between 16 to 31 weeks of intra uterine life (IUL). Twenty human fetuses...

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Autores principales:	Simões,Mariane Cristina Donato, Agopian,Rafael Gabaret, Cabrita,Henrique Antônio Berwanger de Amorim, Liberti,Edson Aparecido
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2019
Materias:	Anatomy Human fetus Hip joint Chondrocytes Collagen
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022019000401450
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spelling	oai:scielo:S0717-950220190004014502019-09-11Morphofunctional and Ultrastructural Evaluation of Hip Joint Development in Human FetusesSimões,Mariane Cristina DonatoAgopian,Rafael GabaretCabrita,Henrique Antônio Berwanger de AmorimLiberti,Edson Aparecido Anatomy Human fetus Hip joint Chondrocytes Collagen SUMMARY: The normal sequential development of the hip joint (HJ) was considered for the evaluation of the morphological and ultrastructural aspects of the joint cartilage of the proximal femoral head epiphysis in human fetuses between 16 to 31 weeks of intra uterine life (IUL). Twenty human fetuses were fixed in 10 % formalin solution. Fetuses were divided into 4 groups (n=5): Group 1 (G1): 16-19 weeks IUL; Group 2 (G2): 20-23 weeks IUL; Group 3 (G3): 24-27 weeks IUL and Group 4 (G4): 28-31 weeks IUL. The right moieties of the HJ were subjected to light microscopy to determine the chondrocyte area, volume, and density and the extracellular matrix (ECM) density. The collagen component in ECM was qualitatively evaluated using Safranin-O and picrosirius techniques under polarized light. The left portions were analyzed using scanning electron microscopy (SEM). The advance of age revealed a gradual increase in chondrocyte area and volume and in ECM density, and a decrease in chondrocyte density. The apparent prevalence of type II collagen fibers in G1 and type III collagen fibers in G4, as well as a balance between type I and III collagen fibers in G2 and G3 suggest a process of cartilaginous evolution and repair. The pantographic organization of the collagen fiber meshes from the depth to the cartilage surface of the femoral head suggests that the arcade collagen network architecture starts at the fetal stage, regardless of the compressive forces applied. The morphological data may contribute not only to a better understanding of the maturation and cartilage organization in this area but also to serve as a theoretical basis for aspects related to diseases and joint malformations.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.37 n.4 20192019-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022019000401450en10.4067/S0717-95022019000401450
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Anatomy Human fetus Hip joint Chondrocytes Collagen
spellingShingle	Anatomy Human fetus Hip joint Chondrocytes Collagen Simões,Mariane Cristina Donato Agopian,Rafael Gabaret Cabrita,Henrique Antônio Berwanger de Amorim Liberti,Edson Aparecido Morphofunctional and Ultrastructural Evaluation of Hip Joint Development in Human Fetuses
description	SUMMARY: The normal sequential development of the hip joint (HJ) was considered for the evaluation of the morphological and ultrastructural aspects of the joint cartilage of the proximal femoral head epiphysis in human fetuses between 16 to 31 weeks of intra uterine life (IUL). Twenty human fetuses were fixed in 10 % formalin solution. Fetuses were divided into 4 groups (n=5): Group 1 (G1): 16-19 weeks IUL; Group 2 (G2): 20-23 weeks IUL; Group 3 (G3): 24-27 weeks IUL and Group 4 (G4): 28-31 weeks IUL. The right moieties of the HJ were subjected to light microscopy to determine the chondrocyte area, volume, and density and the extracellular matrix (ECM) density. The collagen component in ECM was qualitatively evaluated using Safranin-O and picrosirius techniques under polarized light. The left portions were analyzed using scanning electron microscopy (SEM). The advance of age revealed a gradual increase in chondrocyte area and volume and in ECM density, and a decrease in chondrocyte density. The apparent prevalence of type II collagen fibers in G1 and type III collagen fibers in G4, as well as a balance between type I and III collagen fibers in G2 and G3 suggest a process of cartilaginous evolution and repair. The pantographic organization of the collagen fiber meshes from the depth to the cartilage surface of the femoral head suggests that the arcade collagen network architecture starts at the fetal stage, regardless of the compressive forces applied. The morphological data may contribute not only to a better understanding of the maturation and cartilage organization in this area but also to serve as a theoretical basis for aspects related to diseases and joint malformations.
author	Simões,Mariane Cristina Donato Agopian,Rafael Gabaret Cabrita,Henrique Antônio Berwanger de Amorim Liberti,Edson Aparecido
author_facet	Simões,Mariane Cristina Donato Agopian,Rafael Gabaret Cabrita,Henrique Antônio Berwanger de Amorim Liberti,Edson Aparecido
author_sort	Simões,Mariane Cristina Donato
title	Morphofunctional and Ultrastructural Evaluation of Hip Joint Development in Human Fetuses
title_short	Morphofunctional and Ultrastructural Evaluation of Hip Joint Development in Human Fetuses
title_full	Morphofunctional and Ultrastructural Evaluation of Hip Joint Development in Human Fetuses
title_fullStr	Morphofunctional and Ultrastructural Evaluation of Hip Joint Development in Human Fetuses
title_full_unstemmed	Morphofunctional and Ultrastructural Evaluation of Hip Joint Development in Human Fetuses
title_sort	morphofunctional and ultrastructural evaluation of hip joint development in human fetuses
publisher	Sociedad Chilena de Anatomía
publishDate	2019
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022019000401450
work_keys_str_mv	AT simoesmarianecristinadonato morphofunctionalandultrastructuralevaluationofhipjointdevelopmentinhumanfetuses AT agopianrafaelgabaret morphofunctionalandultrastructuralevaluationofhipjointdevelopmentinhumanfetuses AT cabritahenriqueantonioberwangerdeamorim morphofunctionalandultrastructuralevaluationofhipjointdevelopmentinhumanfetuses AT libertiedsonaparecido morphofunctionalandultrastructuralevaluationofhipjointdevelopmentinhumanfetuses
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Morphofunctional and Ultrastructural Evaluation of Hip Joint Development in Human Fetuses

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