Mimosine Increases the Expressions of Tyrosine Phosphorylated Protein in Mouse Seminal Vesicles

SUMMARY: Acute effect of purified mimosine (MiMo) extracted from Leucaena leucocephala on testicular histopathology has been documented with seminal vesicle (SV) atrophy. Since protein phosphorylation and seminal secretions play important roles in sperm physiology, this study aimed to study the alte...

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Autores principales:	Chaichun,Amnart, Burawat,Jaturon, Arun,Supatcharee, Tongpan,Saranya, Kanla,Pipatpong, Sawatpanich,Tarinee, Iamsaard,Sitthichai
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2019
Materias:	Mimosine (MiMo) Seminal vesicle Tyrosine phosphorylated (TyrPho) protein Mice
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022019000401463
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spelling	oai:scielo:S0717-950220190004014632019-09-11Mimosine Increases the Expressions of Tyrosine Phosphorylated Protein in Mouse Seminal VesiclesChaichun,AmnartBurawat,JaturonArun,SupatchareeTongpan,SaranyaKanla,PipatpongSawatpanich,TarineeIamsaard,Sitthichai Mimosine (MiMo) Seminal vesicle Tyrosine phosphorylated (TyrPho) protein Mice SUMMARY: Acute effect of purified mimosine (MiMo) extracted from Leucaena leucocephala on testicular histopathology has been documented with seminal vesicle (SV) atrophy. Since protein phosphorylation and seminal secretions play important roles in sperm physiology, this study aimed to study the alteration of substances including tyrosine phosphorylated (TyrPho) proteins in seminal vesicle treated with MiMo. Male mice were divided into a control and experimental groups treated with purified MiMo at 3 doses of 15, 30, and 60 mg/KgBW, respectively for 35 consecutive days. The morphology and weights of SV were compared among groups. The levels of magnesium and fructosamine in SV fluid were assayed. The profiles of equally SV total proteins were compared using SDS-PAGE. The expression of seminal TyrPho proteins was detected by western blotting. Recent results showed the decreased weights of SV in MiMo treated mice compared to control. However MiMo in all doses did not affect the levels of magnesium and fructosamine in SV fluid. The SV protein expression of 130 and 55 kDas was obviously decreased in a high dose MiMo. In dose-dependent response, the expressions of 72 and 55 kDas TyrPho proteins of SV were increased. In conclusion, MiMo could affect SV morphological size and protein secretions especially TyrPho proteins.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.37 n.4 20192019-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022019000401463en10.4067/S0717-95022019000401463
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Mimosine (MiMo) Seminal vesicle Tyrosine phosphorylated (TyrPho) protein Mice
spellingShingle	Mimosine (MiMo) Seminal vesicle Tyrosine phosphorylated (TyrPho) protein Mice Chaichun,Amnart Burawat,Jaturon Arun,Supatcharee Tongpan,Saranya Kanla,Pipatpong Sawatpanich,Tarinee Iamsaard,Sitthichai Mimosine Increases the Expressions of Tyrosine Phosphorylated Protein in Mouse Seminal Vesicles
description	SUMMARY: Acute effect of purified mimosine (MiMo) extracted from Leucaena leucocephala on testicular histopathology has been documented with seminal vesicle (SV) atrophy. Since protein phosphorylation and seminal secretions play important roles in sperm physiology, this study aimed to study the alteration of substances including tyrosine phosphorylated (TyrPho) proteins in seminal vesicle treated with MiMo. Male mice were divided into a control and experimental groups treated with purified MiMo at 3 doses of 15, 30, and 60 mg/KgBW, respectively for 35 consecutive days. The morphology and weights of SV were compared among groups. The levels of magnesium and fructosamine in SV fluid were assayed. The profiles of equally SV total proteins were compared using SDS-PAGE. The expression of seminal TyrPho proteins was detected by western blotting. Recent results showed the decreased weights of SV in MiMo treated mice compared to control. However MiMo in all doses did not affect the levels of magnesium and fructosamine in SV fluid. The SV protein expression of 130 and 55 kDas was obviously decreased in a high dose MiMo. In dose-dependent response, the expressions of 72 and 55 kDas TyrPho proteins of SV were increased. In conclusion, MiMo could affect SV morphological size and protein secretions especially TyrPho proteins.
author	Chaichun,Amnart Burawat,Jaturon Arun,Supatcharee Tongpan,Saranya Kanla,Pipatpong Sawatpanich,Tarinee Iamsaard,Sitthichai
author_facet	Chaichun,Amnart Burawat,Jaturon Arun,Supatcharee Tongpan,Saranya Kanla,Pipatpong Sawatpanich,Tarinee Iamsaard,Sitthichai
author_sort	Chaichun,Amnart
title	Mimosine Increases the Expressions of Tyrosine Phosphorylated Protein in Mouse Seminal Vesicles
title_short	Mimosine Increases the Expressions of Tyrosine Phosphorylated Protein in Mouse Seminal Vesicles
title_full	Mimosine Increases the Expressions of Tyrosine Phosphorylated Protein in Mouse Seminal Vesicles
title_fullStr	Mimosine Increases the Expressions of Tyrosine Phosphorylated Protein in Mouse Seminal Vesicles
title_full_unstemmed	Mimosine Increases the Expressions of Tyrosine Phosphorylated Protein in Mouse Seminal Vesicles
title_sort	mimosine increases the expressions of tyrosine phosphorylated protein in mouse seminal vesicles
publisher	Sociedad Chilena de Anatomía
publishDate	2019
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022019000401463
work_keys_str_mv	AT chaichunamnart mimosineincreasestheexpressionsoftyrosinephosphorylatedproteininmouseseminalvesicles AT burawatjaturon mimosineincreasestheexpressionsoftyrosinephosphorylatedproteininmouseseminalvesicles AT arunsupatcharee mimosineincreasestheexpressionsoftyrosinephosphorylatedproteininmouseseminalvesicles AT tongpansaranya mimosineincreasestheexpressionsoftyrosinephosphorylatedproteininmouseseminalvesicles AT kanlapipatpong mimosineincreasestheexpressionsoftyrosinephosphorylatedproteininmouseseminalvesicles AT sawatpanichtarinee mimosineincreasestheexpressionsoftyrosinephosphorylatedproteininmouseseminalvesicles AT iamsaardsitthichai mimosineincreasestheexpressionsoftyrosinephosphorylatedproteininmouseseminalvesicles
_version_	1718445124106584064

Mimosine Increases the Expressions of Tyrosine Phosphorylated Protein in Mouse Seminal Vesicles

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