Commentary on Mitochondrial Stereology in Transmission Electron Microscopy

SUMMARY: Mitochondria (m) are responsible for the energy availability of cells, and their analysis is indicated for example, in studies related to metabolism and oxidative stress. The direct measurement of mitochondria (morphometry) is biased because of the section obliquity and position relative to...

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Autores principales:	Reis-Barbosa,Pedro Henrique, de-Carvalho,Jorge José, del Sol,Mariano, Mandarim-de-Lacerda,Carlos Alberto
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2020
Materias:	Mitochondria Morphometry Stereology Microscopy Cell biology
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022020000100026
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spelling	oai:scielo:S0717-950220200001000262019-11-22Commentary on Mitochondrial Stereology in Transmission Electron MicroscopyReis-Barbosa,Pedro Henriquede-Carvalho,Jorge Josédel Sol,MarianoMandarim-de-Lacerda,Carlos Alberto Mitochondria Morphometry Stereology Microscopy Cell biology SUMMARY: Mitochondria (m) are responsible for the energy availability of cells, and their analysis is indicated for example, in studies related to metabolism and oxidative stress. The direct measurement of mitochondria (morphometry) is biased because of the section obliquity and position relative to the mitochondria length (non-equatorial cut). Therefore, stereology is an appropriate technique to evaluate mitochondria. However, before beginning the study, it is necessary to consider the premises to obtain random and uniform samples to be analyzed stereology. Mitochondria must have the chance to appear in all the possibilities of cut and orientation in the micrographs. The number of micrographs to be analyzed will depend on the distribution and occupation of mitochondria in the cell. After this is resolved, a proposal is the estimation of the following stereological data: volume density (Vv), surface density (Sv), and mean cross-sectional area (A). Overlapping a known test area at each micrograph, the density by area of mitochondria is estimated (NAT). Vv [m] can easily be estimated by point-counting (Vv = Pp/PT; Pp are the points hitting the structure, PT are the number of points of the test system). Sv is estimated overlaying a test-line (LT) on the micrographs and counting the intersections of the lines (I) with the outer membrane (om), inner membrane (im), and crests (c), thus, Sv [om], Sv [im], Sv [c] (Sv = 2I / LT). A [m] is obtained as the ratio: A = Vv / 2NAT.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.38 n.1 20202020-02-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022020000100026en10.4067/S0717-95022020000100026
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Mitochondria Morphometry Stereology Microscopy Cell biology
spellingShingle	Mitochondria Morphometry Stereology Microscopy Cell biology Reis-Barbosa,Pedro Henrique de-Carvalho,Jorge José del Sol,Mariano Mandarim-de-Lacerda,Carlos Alberto Commentary on Mitochondrial Stereology in Transmission Electron Microscopy
description	SUMMARY: Mitochondria (m) are responsible for the energy availability of cells, and their analysis is indicated for example, in studies related to metabolism and oxidative stress. The direct measurement of mitochondria (morphometry) is biased because of the section obliquity and position relative to the mitochondria length (non-equatorial cut). Therefore, stereology is an appropriate technique to evaluate mitochondria. However, before beginning the study, it is necessary to consider the premises to obtain random and uniform samples to be analyzed stereology. Mitochondria must have the chance to appear in all the possibilities of cut and orientation in the micrographs. The number of micrographs to be analyzed will depend on the distribution and occupation of mitochondria in the cell. After this is resolved, a proposal is the estimation of the following stereological data: volume density (Vv), surface density (Sv), and mean cross-sectional area (A). Overlapping a known test area at each micrograph, the density by area of mitochondria is estimated (NAT). Vv [m] can easily be estimated by point-counting (Vv = Pp/PT; Pp are the points hitting the structure, PT are the number of points of the test system). Sv is estimated overlaying a test-line (LT) on the micrographs and counting the intersections of the lines (I) with the outer membrane (om), inner membrane (im), and crests (c), thus, Sv [om], Sv [im], Sv [c] (Sv = 2I / LT). A [m] is obtained as the ratio: A = Vv / 2NAT.
author	Reis-Barbosa,Pedro Henrique de-Carvalho,Jorge José del Sol,Mariano Mandarim-de-Lacerda,Carlos Alberto
author_facet	Reis-Barbosa,Pedro Henrique de-Carvalho,Jorge José del Sol,Mariano Mandarim-de-Lacerda,Carlos Alberto
author_sort	Reis-Barbosa,Pedro Henrique
title	Commentary on Mitochondrial Stereology in Transmission Electron Microscopy
title_short	Commentary on Mitochondrial Stereology in Transmission Electron Microscopy
title_full	Commentary on Mitochondrial Stereology in Transmission Electron Microscopy
title_fullStr	Commentary on Mitochondrial Stereology in Transmission Electron Microscopy
title_full_unstemmed	Commentary on Mitochondrial Stereology in Transmission Electron Microscopy
title_sort	commentary on mitochondrial stereology in transmission electron microscopy
publisher	Sociedad Chilena de Anatomía
publishDate	2020
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022020000100026
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Commentary on Mitochondrial Stereology in Transmission Electron Microscopy

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