Suppression of Hepatic Apoptosis Induced by Acetaminophen Using a Combination of Resveratrol and Quercetin: An Association of Oxidative Stress and Interleukin-11
SUMMARY: We sought to determine whether the combined polyphenolic compounds, resveratrol and quercetin can substantially protect against modulation of hepatic biomarkers of apoptosis and survival, p53-Bax axis and B-cell lymphoma 2 (Bcl-2) in an animal model of acetaminophen-induced acute liver inju...
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Sociedad Chilena de Anatomía
2020
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oai:scielo:S0717-950220200001000832019-11-22Suppression of Hepatic Apoptosis Induced by Acetaminophen Using a Combination of Resveratrol and Quercetin: An Association of Oxidative Stress and Interleukin-11Al-Humayed,SulimanAl-Ani,BahjatShatoor,Abdullah SEl-Karib,Abbas ODallak,MohammadKamar,Samaa SHaidara,Mohamed A Acetaminophen Acute liver injury Apoptosis, Resveratrol Quercetin Rat model SUMMARY: We sought to determine whether the combined polyphenolic compounds, resveratrol and quercetin can substantially protect against modulation of hepatic biomarkers of apoptosis and survival, p53-Bax axis and B-cell lymphoma 2 (Bcl-2) in an animal model of acetaminophen-induced acute liver injury via the association of oxidative stress and interleukin-11 (IL-11). The model group of rats received a single dose of acetaminophen (2 g/kg), whereas the protective group of rats was pre-treated for 7 days with combined doses of resveratrol (30 mg/kg) and quercetin (50 mg/kg) before being given a single dose of acetaminophen. All rats were then sacrificed 24 hours post acetaminophen ingestion. Acetaminophen overdose induced acute liver injury as demonstrated by profound liver parenchymal damage and increased levels of the liver injury enzymes, alanine aminotransferase (ALT) and aspartate aminotransferase (AST). Acetaminophen significantly (p<0.05) modulated malondialdehyde (MDA), p53, apoptosis regulator Bax, Bcl-2, IL-11, interleukin-6 (IL-6), ALT, AST, superoxide dismutase (SOD), and glutathione peroxidase (GPx), which were significantly protected by resveratrol plus quercetin. We further demonstrated a significant (p<0.01) correlation between IL-11 scoring and the levels of p53, Bax, Bcl-2, and MDA. Thus, resveratrol plus quercetin effectively protect against acetaminophen-induced apoptosis, which is associated with the inhibition of oxidative stress and IL-11.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.38 n.1 20202020-02-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022020000100083en10.4067/S0717-95022020000100083 |
| institution |
Scielo Chile |
| collection |
Scielo Chile |
| language |
English |
| topic |
Acetaminophen Acute liver injury Apoptosis, Resveratrol Quercetin Rat model |
| spellingShingle |
Acetaminophen Acute liver injury Apoptosis, Resveratrol Quercetin Rat model Al-Humayed,Suliman Al-Ani,Bahjat Shatoor,Abdullah S El-Karib,Abbas O Dallak,Mohammad Kamar,Samaa S Haidara,Mohamed A Suppression of Hepatic Apoptosis Induced by Acetaminophen Using a Combination of Resveratrol and Quercetin: An Association of Oxidative Stress and Interleukin-11 |
| description |
SUMMARY: We sought to determine whether the combined polyphenolic compounds, resveratrol and quercetin can substantially protect against modulation of hepatic biomarkers of apoptosis and survival, p53-Bax axis and B-cell lymphoma 2 (Bcl-2) in an animal model of acetaminophen-induced acute liver injury via the association of oxidative stress and interleukin-11 (IL-11). The model group of rats received a single dose of acetaminophen (2 g/kg), whereas the protective group of rats was pre-treated for 7 days with combined doses of resveratrol (30 mg/kg) and quercetin (50 mg/kg) before being given a single dose of acetaminophen. All rats were then sacrificed 24 hours post acetaminophen ingestion. Acetaminophen overdose induced acute liver injury as demonstrated by profound liver parenchymal damage and increased levels of the liver injury enzymes, alanine aminotransferase (ALT) and aspartate aminotransferase (AST). Acetaminophen significantly (p<0.05) modulated malondialdehyde (MDA), p53, apoptosis regulator Bax, Bcl-2, IL-11, interleukin-6 (IL-6), ALT, AST, superoxide dismutase (SOD), and glutathione peroxidase (GPx), which were significantly protected by resveratrol plus quercetin. We further demonstrated a significant (p<0.01) correlation between IL-11 scoring and the levels of p53, Bax, Bcl-2, and MDA. Thus, resveratrol plus quercetin effectively protect against acetaminophen-induced apoptosis, which is associated with the inhibition of oxidative stress and IL-11. |
| author |
Al-Humayed,Suliman Al-Ani,Bahjat Shatoor,Abdullah S El-Karib,Abbas O Dallak,Mohammad Kamar,Samaa S Haidara,Mohamed A |
| author_facet |
Al-Humayed,Suliman Al-Ani,Bahjat Shatoor,Abdullah S El-Karib,Abbas O Dallak,Mohammad Kamar,Samaa S Haidara,Mohamed A |
| author_sort |
Al-Humayed,Suliman |
| title |
Suppression of Hepatic Apoptosis Induced by Acetaminophen Using a Combination of Resveratrol and Quercetin: An Association of Oxidative Stress and Interleukin-11 |
| title_short |
Suppression of Hepatic Apoptosis Induced by Acetaminophen Using a Combination of Resveratrol and Quercetin: An Association of Oxidative Stress and Interleukin-11 |
| title_full |
Suppression of Hepatic Apoptosis Induced by Acetaminophen Using a Combination of Resveratrol and Quercetin: An Association of Oxidative Stress and Interleukin-11 |
| title_fullStr |
Suppression of Hepatic Apoptosis Induced by Acetaminophen Using a Combination of Resveratrol and Quercetin: An Association of Oxidative Stress and Interleukin-11 |
| title_full_unstemmed |
Suppression of Hepatic Apoptosis Induced by Acetaminophen Using a Combination of Resveratrol and Quercetin: An Association of Oxidative Stress and Interleukin-11 |
| title_sort |
suppression of hepatic apoptosis induced by acetaminophen using a combination of resveratrol and quercetin: an association of oxidative stress and interleukin-11 |
| publisher |
Sociedad Chilena de Anatomía |
| publishDate |
2020 |
| url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022020000100083 |
| work_keys_str_mv |
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