Brain Structure Morphology After Being Fixated With Ethanol on Electron Microscope

SUMMARY: Fixation is one of the processes in preparing histology and pathology. The common material for fixation is buffered formalin including paraformaldehyde. However, the effect of the damaged cells, which is fixed for a long time, causes the research for other fixation materials to become neces...

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Autores principales:	Dewi,Arni Kusuma, Anwar,Chairul, Komohara,Yoshihiro
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2020
Materias:	Fixation Brain Organelle Electron Microscope.
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022020000200305
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spelling	oai:scielo:S0717-950220200002003052020-02-03Brain Structure Morphology After Being Fixated With Ethanol on Electron MicroscopeDewi,Arni KusumaAnwar,ChairulKomohara,Yoshihiro Fixation Brain Organelle Electron Microscope. SUMMARY: Fixation is one of the processes in preparing histology and pathology. The common material for fixation is buffered formalin including paraformaldehyde. However, the effect of the damaged cells, which is fixed for a long time, causes the research for other fixation materials to become necessary. In addition, paraformaldehyde is also harmful to human body and natural environment. Ethanol is one of the alternative fixation materials, which has been used for two hundred years. It has been used for many purposes, both in routine staining and immunohistochemistry. Nonetheless, no research confirms its effect on the electron microscope. The authors studied the effect of 50 % of ethanol on the cell membrane, organelles, and nucleus of Purkinje cells (Neuron purkinjense) observed on a light microscope and Transmitted Electron Microscope (TEM). Then it was compared to buffered formalin. In the light microscope, it shows that both of fixations have no different effects of the morphology of the cell membrane, cytoplasm, the nucleus of Purkinje cells and the neutrophils. We assume that our 50 % of ethanol concentration is almost the same as BF 10 % in the ability of hardening tissue and color absorption based on the previous study. In TEM, the structure of the cell membrane, organelles, and cytoplasm of Purkinje cell look broken in the cerebellum of 50 % of ethanol except for the nucleus. There was no significant difference diameter of the nucleus. It happened in general because of the shrinkage effect of ethanol. However, the authors recommend using 50 % of ethanol for routine staining.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.38 n.2 20202020-04-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022020000200305en10.4067/S0717-95022020000200305
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Fixation Brain Organelle Electron Microscope.
spellingShingle	Fixation Brain Organelle Electron Microscope. Dewi,Arni Kusuma Anwar,Chairul Komohara,Yoshihiro Brain Structure Morphology After Being Fixated With Ethanol on Electron Microscope
description	SUMMARY: Fixation is one of the processes in preparing histology and pathology. The common material for fixation is buffered formalin including paraformaldehyde. However, the effect of the damaged cells, which is fixed for a long time, causes the research for other fixation materials to become necessary. In addition, paraformaldehyde is also harmful to human body and natural environment. Ethanol is one of the alternative fixation materials, which has been used for two hundred years. It has been used for many purposes, both in routine staining and immunohistochemistry. Nonetheless, no research confirms its effect on the electron microscope. The authors studied the effect of 50 % of ethanol on the cell membrane, organelles, and nucleus of Purkinje cells (Neuron purkinjense) observed on a light microscope and Transmitted Electron Microscope (TEM). Then it was compared to buffered formalin. In the light microscope, it shows that both of fixations have no different effects of the morphology of the cell membrane, cytoplasm, the nucleus of Purkinje cells and the neutrophils. We assume that our 50 % of ethanol concentration is almost the same as BF 10 % in the ability of hardening tissue and color absorption based on the previous study. In TEM, the structure of the cell membrane, organelles, and cytoplasm of Purkinje cell look broken in the cerebellum of 50 % of ethanol except for the nucleus. There was no significant difference diameter of the nucleus. It happened in general because of the shrinkage effect of ethanol. However, the authors recommend using 50 % of ethanol for routine staining.
author	Dewi,Arni Kusuma Anwar,Chairul Komohara,Yoshihiro
author_facet	Dewi,Arni Kusuma Anwar,Chairul Komohara,Yoshihiro
author_sort	Dewi,Arni Kusuma
title	Brain Structure Morphology After Being Fixated With Ethanol on Electron Microscope
title_short	Brain Structure Morphology After Being Fixated With Ethanol on Electron Microscope
title_full	Brain Structure Morphology After Being Fixated With Ethanol on Electron Microscope
title_fullStr	Brain Structure Morphology After Being Fixated With Ethanol on Electron Microscope
title_full_unstemmed	Brain Structure Morphology After Being Fixated With Ethanol on Electron Microscope
title_sort	brain structure morphology after being fixated with ethanol on electron microscope
publisher	Sociedad Chilena de Anatomía
publishDate	2020
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022020000200305
work_keys_str_mv	AT dewiarnikusuma brainstructuremorphologyafterbeingfixatedwithethanolonelectronmicroscope AT anwarchairul brainstructuremorphologyafterbeingfixatedwithethanolonelectronmicroscope AT komoharayoshihiro brainstructuremorphologyafterbeingfixatedwithethanolonelectronmicroscope
_version_	1718445136788062208

Brain Structure Morphology After Being Fixated With Ethanol on Electron Microscope

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