Ameliorative Effect of Vitamin E on Biochemical and Ultrastructural Changes in Artemether-induced Renal Toxicity in Rats

SUMMARY: This experiment was designed to study the administration of normal doses of one of recent antimalarial drug and coadministration of vitamin E on the kidney tissue. A total twenty-four adult male albino rats were used and divided into four groups: the first one served as a control, the secon...

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Autores principales: Eid,Refaat A, Zaki,Mohamed Samir Ahmed, Alghamd,Mansour A, Wares,Adnan, Eldeen,Muhammad Alaa, Massoud,Ehab El Sayed, Haidara,Mohamed A
Lenguaje:English
Publicado: Sociedad Chilena de Anatomía 2020
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Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022020000200461
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spelling oai:scielo:S0717-950220200002004612020-02-03Ameliorative Effect of Vitamin E on Biochemical and Ultrastructural Changes in Artemether-induced Renal Toxicity in RatsEid,Refaat AZaki,Mohamed Samir AhmedAlghamd,Mansour AWares,AdnanEldeen,Muhammad AlaaMassoud,Ehab El SayedHaidara,Mohamed A Artemether Rats Vitamin E Light and electron microscopy Biomarkers of kidney injury SUMMARY: This experiment was designed to study the administration of normal doses of one of recent antimalarial drug and coadministration of vitamin E on the kidney tissue. A total twenty-four adult male albino rats were used and divided into four groups: the first one served as a control, the second received artemether orally for three days consecutively. The rats of the third and fourth groups received the same dose of artemether concomitantly with 50 and 100 mg/kg vitamin E orally daily for 2 weeks. After the last dose, the rats were sacrificed and the kidney tissues with blood samples obtained and processed for light, electron microscopic and biochemical analysis. Histologically, artemether treated kidneys showed atrophied glomeruli with widened urinary space and kidney tubules were degenerated with disturbed contour and some vacuoles inside it. Ultrastructurally, the glomeruli of this group showed hypertrophic endothelial cells, irregularity of its basement membrane, disrupted foot processes and filtration slits. The kidney tubule cells showed loss of basal infoldings, cytoplasmic vacuolation, polymorphic damaged swollen mitochondria a loss of its microvilli towards its capillary lumen. Artemether plus vitamin E of the rat kidney groups showed improvement of morphological changes compared to the changes seen in artemether alone. These data were confirmed by biochemical findings with marked improvement of blood urea and creatinine levels and increase of anti-oxidant enzyme activities of glutathione peroxidase and superoxide dismutase in the vitamin E treated groups. The results of this study revealed that vitamins E can improve the adverse changes of artemether of rat renal tissue.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.38 n.2 20202020-04-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022020000200461en10.4067/S0717-95022020000200461
institution Scielo Chile
collection Scielo Chile
language English
topic Artemether
Rats
Vitamin E
Light and electron microscopy
Biomarkers of kidney injury
spellingShingle Artemether
Rats
Vitamin E
Light and electron microscopy
Biomarkers of kidney injury
Eid,Refaat A
Zaki,Mohamed Samir Ahmed
Alghamd,Mansour A
Wares,Adnan
Eldeen,Muhammad Alaa
Massoud,Ehab El Sayed
Haidara,Mohamed A
Ameliorative Effect of Vitamin E on Biochemical and Ultrastructural Changes in Artemether-induced Renal Toxicity in Rats
description SUMMARY: This experiment was designed to study the administration of normal doses of one of recent antimalarial drug and coadministration of vitamin E on the kidney tissue. A total twenty-four adult male albino rats were used and divided into four groups: the first one served as a control, the second received artemether orally for three days consecutively. The rats of the third and fourth groups received the same dose of artemether concomitantly with 50 and 100 mg/kg vitamin E orally daily for 2 weeks. After the last dose, the rats were sacrificed and the kidney tissues with blood samples obtained and processed for light, electron microscopic and biochemical analysis. Histologically, artemether treated kidneys showed atrophied glomeruli with widened urinary space and kidney tubules were degenerated with disturbed contour and some vacuoles inside it. Ultrastructurally, the glomeruli of this group showed hypertrophic endothelial cells, irregularity of its basement membrane, disrupted foot processes and filtration slits. The kidney tubule cells showed loss of basal infoldings, cytoplasmic vacuolation, polymorphic damaged swollen mitochondria a loss of its microvilli towards its capillary lumen. Artemether plus vitamin E of the rat kidney groups showed improvement of morphological changes compared to the changes seen in artemether alone. These data were confirmed by biochemical findings with marked improvement of blood urea and creatinine levels and increase of anti-oxidant enzyme activities of glutathione peroxidase and superoxide dismutase in the vitamin E treated groups. The results of this study revealed that vitamins E can improve the adverse changes of artemether of rat renal tissue.
author Eid,Refaat A
Zaki,Mohamed Samir Ahmed
Alghamd,Mansour A
Wares,Adnan
Eldeen,Muhammad Alaa
Massoud,Ehab El Sayed
Haidara,Mohamed A
author_facet Eid,Refaat A
Zaki,Mohamed Samir Ahmed
Alghamd,Mansour A
Wares,Adnan
Eldeen,Muhammad Alaa
Massoud,Ehab El Sayed
Haidara,Mohamed A
author_sort Eid,Refaat A
title Ameliorative Effect of Vitamin E on Biochemical and Ultrastructural Changes in Artemether-induced Renal Toxicity in Rats
title_short Ameliorative Effect of Vitamin E on Biochemical and Ultrastructural Changes in Artemether-induced Renal Toxicity in Rats
title_full Ameliorative Effect of Vitamin E on Biochemical and Ultrastructural Changes in Artemether-induced Renal Toxicity in Rats
title_fullStr Ameliorative Effect of Vitamin E on Biochemical and Ultrastructural Changes in Artemether-induced Renal Toxicity in Rats
title_full_unstemmed Ameliorative Effect of Vitamin E on Biochemical and Ultrastructural Changes in Artemether-induced Renal Toxicity in Rats
title_sort ameliorative effect of vitamin e on biochemical and ultrastructural changes in artemether-induced renal toxicity in rats
publisher Sociedad Chilena de Anatomía
publishDate 2020
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022020000200461
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