Autophagy Inhibitor 3-Methyladenine could not Modulate Rotenone Neurotoxicity in Primary Mesencephalic Cell Culture

SUMMARY: Dysregulated autophagy, whether excessive or downregulated, has been thought to be associated with neurodegenerative disorders including Parkinson’s disease. Accordingly, the present study was carried out to investigate whether 3-methyladenine, an autophagy inhibitor, can modulate...

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Autores principales:	Radad,Khaled, Al-Shraim,Mubarak, Al-Emam,Ahmed, Rausch,Wolf-Dieter, Moldzio,Rudolf
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2020
Materias:	Autophagy Dopaminergic neurons Parkinson’s disease Rotenone 3-methyladenine
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022020000300530
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spelling	oai:scielo:S0717-950220200003005302020-04-07Autophagy Inhibitor 3-Methyladenine could not Modulate Rotenone Neurotoxicity in Primary Mesencephalic Cell CultureRadad,KhaledAl-Shraim,MubarakAl-Emam,AhmedRausch,Wolf-DieterMoldzio,Rudolf Autophagy Dopaminergic neurons Parkinson’s disease Rotenone 3-methyladenine SUMMARY: Dysregulated autophagy, whether excessive or downregulated, has been thought to be associated with neurodegenerative disorders including Parkinson’s disease. Accordingly, the present study was carried out to investigate whether 3-methyladenine, an autophagy inhibitor, can modulate the effects of rotenone on dopaminergic neurons in primary mesencephalic cell culture. Cultures were prepared from embryonic mouse mesencephala at gestation day 14. Four groups of cultures were treated on the 10th DIV for 48 h as follows: the first was kept as an untreated control, the second was treated with 3-methyladenine alone (1, 10, 100, 200 mM), the third was treated with 20 nM rotenone and the fourth was co-treated with 20 nM rotenone and 3-methyladenine (1, 10, 100, 200 mM). On the 12th DIV, cultured cells were stained immunohistochemically against tyrosine hydroxylase and culture media were used to measure the levels of lactate dehydrogenase. 3methyladenine had no effects on both the survival of dopaminergic neurons and the release of lactate dehydrogenase. Rotenone significantly decreased the number of dopaminergic neurons and increased the levels of lactate dehydrogenase in the culture media. When cultures concomitantly treated with 3-methyladenine and rotenone, 3-methyladenine had no effect against rotenone-induced dopaminergic cell damage and lactate dehydrogenase release into the culture medium. In conclusion, the autophagy inhibitor 3-methyladenine could not modulate rotenone-induced dopaminergic cell damage in primary mesencephalic cell culture.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.38 n.3 20202020-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022020000300530en10.4067/S0717-95022020000300530
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Autophagy Dopaminergic neurons Parkinson’s disease Rotenone 3-methyladenine
spellingShingle	Autophagy Dopaminergic neurons Parkinson’s disease Rotenone 3-methyladenine Radad,Khaled Al-Shraim,Mubarak Al-Emam,Ahmed Rausch,Wolf-Dieter Moldzio,Rudolf Autophagy Inhibitor 3-Methyladenine could not Modulate Rotenone Neurotoxicity in Primary Mesencephalic Cell Culture
description	SUMMARY: Dysregulated autophagy, whether excessive or downregulated, has been thought to be associated with neurodegenerative disorders including Parkinson’s disease. Accordingly, the present study was carried out to investigate whether 3-methyladenine, an autophagy inhibitor, can modulate the effects of rotenone on dopaminergic neurons in primary mesencephalic cell culture. Cultures were prepared from embryonic mouse mesencephala at gestation day 14. Four groups of cultures were treated on the 10th DIV for 48 h as follows: the first was kept as an untreated control, the second was treated with 3-methyladenine alone (1, 10, 100, 200 mM), the third was treated with 20 nM rotenone and the fourth was co-treated with 20 nM rotenone and 3-methyladenine (1, 10, 100, 200 mM). On the 12th DIV, cultured cells were stained immunohistochemically against tyrosine hydroxylase and culture media were used to measure the levels of lactate dehydrogenase. 3methyladenine had no effects on both the survival of dopaminergic neurons and the release of lactate dehydrogenase. Rotenone significantly decreased the number of dopaminergic neurons and increased the levels of lactate dehydrogenase in the culture media. When cultures concomitantly treated with 3-methyladenine and rotenone, 3-methyladenine had no effect against rotenone-induced dopaminergic cell damage and lactate dehydrogenase release into the culture medium. In conclusion, the autophagy inhibitor 3-methyladenine could not modulate rotenone-induced dopaminergic cell damage in primary mesencephalic cell culture.
author	Radad,Khaled Al-Shraim,Mubarak Al-Emam,Ahmed Rausch,Wolf-Dieter Moldzio,Rudolf
author_facet	Radad,Khaled Al-Shraim,Mubarak Al-Emam,Ahmed Rausch,Wolf-Dieter Moldzio,Rudolf
author_sort	Radad,Khaled
title	Autophagy Inhibitor 3-Methyladenine could not Modulate Rotenone Neurotoxicity in Primary Mesencephalic Cell Culture
title_short	Autophagy Inhibitor 3-Methyladenine could not Modulate Rotenone Neurotoxicity in Primary Mesencephalic Cell Culture
title_full	Autophagy Inhibitor 3-Methyladenine could not Modulate Rotenone Neurotoxicity in Primary Mesencephalic Cell Culture
title_fullStr	Autophagy Inhibitor 3-Methyladenine could not Modulate Rotenone Neurotoxicity in Primary Mesencephalic Cell Culture
title_full_unstemmed	Autophagy Inhibitor 3-Methyladenine could not Modulate Rotenone Neurotoxicity in Primary Mesencephalic Cell Culture
title_sort	autophagy inhibitor 3-methyladenine could not modulate rotenone neurotoxicity in primary mesencephalic cell culture
publisher	Sociedad Chilena de Anatomía
publishDate	2020
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022020000300530
work_keys_str_mv	AT radadkhaled autophagyinhibitor3methyladeninecouldnotmodulaterotenoneneurotoxicityinprimarymesencephaliccellculture AT alshraimmubarak autophagyinhibitor3methyladeninecouldnotmodulaterotenoneneurotoxicityinprimarymesencephaliccellculture AT alemamahmed autophagyinhibitor3methyladeninecouldnotmodulaterotenoneneurotoxicityinprimarymesencephaliccellculture AT rauschwolfdieter autophagyinhibitor3methyladeninecouldnotmodulaterotenoneneurotoxicityinprimarymesencephaliccellculture AT moldziorudolf autophagyinhibitor3methyladeninecouldnotmodulaterotenoneneurotoxicityinprimarymesencephaliccellculture
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Autophagy Inhibitor 3-Methyladenine could not Modulate Rotenone Neurotoxicity in Primary Mesencephalic Cell Culture

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