Acetaminophen Induces Alterations to the Renal Tubular Ultrastructure in a Rat Model of Acute Nephrotoxicity Protected by Resveratrol and Quercetin

SUMMARY: Acetaminophen (also called paracetamol, or APAP) induced nephrotoxicity is reported after accidental or intentional ingestion of an overdose of the drug. Renal tubular ultrastructural alterations induced by APAP overdose associated with the induction of biomarkers of kidney injury have not...

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Autores principales:	Haidara,Mohamed A, Al-Ani,Bahjat, Eid,Refaat A, Mohammed,Muataz E. D, Al-Hashem,Fahaid, Dallak,Mohammad
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2020
Materias:	Renal tubule ultrastructure Acetaminophen Resveratrol Quercetin Rat model
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022020000300585
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spelling	oai:scielo:S0717-950220200003005852020-04-07Acetaminophen Induces Alterations to the Renal Tubular Ultrastructure in a Rat Model of Acute Nephrotoxicity Protected by Resveratrol and QuercetinHaidara,Mohamed AAl-Ani,BahjatEid,Refaat AMohammed,Muataz E. DAl-Hashem,FahaidDallak,Mohammad Renal tubule ultrastructure Acetaminophen Resveratrol Quercetin Rat model SUMMARY: Acetaminophen (also called paracetamol, or APAP) induced nephrotoxicity is reported after accidental or intentional ingestion of an overdose of the drug. Renal tubular ultrastructural alterations induced by APAP overdose associated with the induction of biomarkers of kidney injury have not been investigated before. Also, we investigated whether the combined polyphenolic anti-inflammatory and antioxidants agents, resveratrol (RES) and quercetin (QUR) can protect against APAP-induced acute kidney injury. The model group of rats received a single dose of APAP (2 g/kg), whereas the protective group of rats was pre-treated for 7 days with combined doses of RES (30 mg/kg) and QUR (50 mg/kg) before being given a single dose of APAP. All rats were then sacrificed one day post APAP ingestion. Harvested kidney tissues were prepared for transmission electron microscopy (TEM) staining and blood samples were assayed for urea, creatinine, and biomarkers of inflammation and oxidative stress. TEM images and blood chemistry analysis showed that APAP overdose induced kidney damage as demonstrated by substantial alterations to the proximal convoluted tubule ultrastructure, and a significant (p<0.05) increase in urea, creatinine, tumor necrosis factor-alpha (TNF-a), and malondialdehyde (MDA) blood levels, which were protected by RES+QUR. These findings indicate that APAP induces alterations to the renal tubular ultrastructure, which is inhibited by resveratrol plus quercetin, which also decreases blood levels of kidney injury biomarkers.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.38 n.3 20202020-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022020000300585en10.4067/S0717-95022020000300585
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Renal tubule ultrastructure Acetaminophen Resveratrol Quercetin Rat model
spellingShingle	Renal tubule ultrastructure Acetaminophen Resveratrol Quercetin Rat model Haidara,Mohamed A Al-Ani,Bahjat Eid,Refaat A Mohammed,Muataz E. D Al-Hashem,Fahaid Dallak,Mohammad Acetaminophen Induces Alterations to the Renal Tubular Ultrastructure in a Rat Model of Acute Nephrotoxicity Protected by Resveratrol and Quercetin
description	SUMMARY: Acetaminophen (also called paracetamol, or APAP) induced nephrotoxicity is reported after accidental or intentional ingestion of an overdose of the drug. Renal tubular ultrastructural alterations induced by APAP overdose associated with the induction of biomarkers of kidney injury have not been investigated before. Also, we investigated whether the combined polyphenolic anti-inflammatory and antioxidants agents, resveratrol (RES) and quercetin (QUR) can protect against APAP-induced acute kidney injury. The model group of rats received a single dose of APAP (2 g/kg), whereas the protective group of rats was pre-treated for 7 days with combined doses of RES (30 mg/kg) and QUR (50 mg/kg) before being given a single dose of APAP. All rats were then sacrificed one day post APAP ingestion. Harvested kidney tissues were prepared for transmission electron microscopy (TEM) staining and blood samples were assayed for urea, creatinine, and biomarkers of inflammation and oxidative stress. TEM images and blood chemistry analysis showed that APAP overdose induced kidney damage as demonstrated by substantial alterations to the proximal convoluted tubule ultrastructure, and a significant (p<0.05) increase in urea, creatinine, tumor necrosis factor-alpha (TNF-a), and malondialdehyde (MDA) blood levels, which were protected by RES+QUR. These findings indicate that APAP induces alterations to the renal tubular ultrastructure, which is inhibited by resveratrol plus quercetin, which also decreases blood levels of kidney injury biomarkers.
author	Haidara,Mohamed A Al-Ani,Bahjat Eid,Refaat A Mohammed,Muataz E. D Al-Hashem,Fahaid Dallak,Mohammad
author_facet	Haidara,Mohamed A Al-Ani,Bahjat Eid,Refaat A Mohammed,Muataz E. D Al-Hashem,Fahaid Dallak,Mohammad
author_sort	Haidara,Mohamed A
title	Acetaminophen Induces Alterations to the Renal Tubular Ultrastructure in a Rat Model of Acute Nephrotoxicity Protected by Resveratrol and Quercetin
title_short	Acetaminophen Induces Alterations to the Renal Tubular Ultrastructure in a Rat Model of Acute Nephrotoxicity Protected by Resveratrol and Quercetin
title_full	Acetaminophen Induces Alterations to the Renal Tubular Ultrastructure in a Rat Model of Acute Nephrotoxicity Protected by Resveratrol and Quercetin
title_fullStr	Acetaminophen Induces Alterations to the Renal Tubular Ultrastructure in a Rat Model of Acute Nephrotoxicity Protected by Resveratrol and Quercetin
title_full_unstemmed	Acetaminophen Induces Alterations to the Renal Tubular Ultrastructure in a Rat Model of Acute Nephrotoxicity Protected by Resveratrol and Quercetin
title_sort	acetaminophen induces alterations to the renal tubular ultrastructure in a rat model of acute nephrotoxicity protected by resveratrol and quercetin
publisher	Sociedad Chilena de Anatomía
publishDate	2020
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022020000300585
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Acetaminophen Induces Alterations to the Renal Tubular Ultrastructure in a Rat Model of Acute Nephrotoxicity Protected by Resveratrol and Quercetin

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