Protective Effects of Honey, Apis mellifera Meda Skorikov, on Ischemia-Reperfusion Induced Muscle Injury

SUMMARY: Honey is a natural antioxidant that its protective effects have been proven against ischemia-reperfusion (IR) injury. The aim of this study was to evaluate the ameliorative effect of Persian Honey, Apis mellifera meda skorikov, on gastrocnemius muscle IR injury. Eighty adult male Sprague-Da...

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Autores principales:	Gholami,Mohammad Reza, Abbaszadeh,Abolfazl, Anbari,Khatereh, Khaksarian,Mojtaba, Shabooni,Fatemeh, Khayat,Zahra Khanipour, Khorramabadi,Reza Mohammadrezaei, Gharravi,Anneh Mohammad
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2020
Materias:	Honey Ischemia-reperfusion Injury Gastrocnemius Muscle Rat
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022020000300804
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spelling	oai:scielo:S0717-950220200003008042020-04-07Protective Effects of Honey, Apis mellifera Meda Skorikov, on Ischemia-Reperfusion Induced Muscle InjuryGholami,Mohammad RezaAbbaszadeh,AbolfazlAnbari,KhaterehKhaksarian,MojtabaShabooni,FatemehKhayat,Zahra KhanipourKhorramabadi,Reza MohammadrezaeiGharravi,Anneh Mohammad Honey Ischemia-reperfusion Injury Gastrocnemius Muscle Rat SUMMARY: Honey is a natural antioxidant that its protective effects have been proven against ischemia-reperfusion (IR) injury. The aim of this study was to evaluate the ameliorative effect of Persian Honey, Apis mellifera meda skorikov, on gastrocnemius muscle IR injury. Eighty adult male Sprague-Dawley rats weighing 250-300 g were used. They were divided into ten groups (N=8 per group). The ischemia was conducted with a silk suture 6-0 using the slipknot technique. All groups were rendered in ischemic for 3 h, and reperfused for various times of 3 days (3-day reperfusion), 7 days (7-day reperfusion), 14 days (14-day reperfusion), and 28 days (28-day reperfusion). Half of the groups had experimental honey (5 %) treatment immediately after ischemia. After reperfusion, the rats, based on the grouping, were killed with high doses of anesthetic, and the gastrocnemius muscles were removed and fixed. After the tissue processing, the evaluation of edema and mast cells infiltration was performed with hematoxylin-eosin and toluidine blue staining, respectively. TNF-α was detected with immunohistochemistry method. The amount of TNF-α as an index of acute inflammatory except the 3rd day significantly decreased on the other day of reperfusion (7th, 147th and 287th days). The mast cells infiltration was significantly decreased on 77th and 147th days. The tissue edema was decreased significantly in honey administrated group in the comparison with placebo groups. Honey administration can reduce damage caused by ischemia-reperfusion in the rat gastrocnemius muscle.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.38 n.3 20202020-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022020000300804en10.4067/S0717-95022020000300804
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Honey Ischemia-reperfusion Injury Gastrocnemius Muscle Rat
spellingShingle	Honey Ischemia-reperfusion Injury Gastrocnemius Muscle Rat Gholami,Mohammad Reza Abbaszadeh,Abolfazl Anbari,Khatereh Khaksarian,Mojtaba Shabooni,Fatemeh Khayat,Zahra Khanipour Khorramabadi,Reza Mohammadrezaei Gharravi,Anneh Mohammad Protective Effects of Honey, Apis mellifera Meda Skorikov, on Ischemia-Reperfusion Induced Muscle Injury
description	SUMMARY: Honey is a natural antioxidant that its protective effects have been proven against ischemia-reperfusion (IR) injury. The aim of this study was to evaluate the ameliorative effect of Persian Honey, Apis mellifera meda skorikov, on gastrocnemius muscle IR injury. Eighty adult male Sprague-Dawley rats weighing 250-300 g were used. They were divided into ten groups (N=8 per group). The ischemia was conducted with a silk suture 6-0 using the slipknot technique. All groups were rendered in ischemic for 3 h, and reperfused for various times of 3 days (3-day reperfusion), 7 days (7-day reperfusion), 14 days (14-day reperfusion), and 28 days (28-day reperfusion). Half of the groups had experimental honey (5 %) treatment immediately after ischemia. After reperfusion, the rats, based on the grouping, were killed with high doses of anesthetic, and the gastrocnemius muscles were removed and fixed. After the tissue processing, the evaluation of edema and mast cells infiltration was performed with hematoxylin-eosin and toluidine blue staining, respectively. TNF-α was detected with immunohistochemistry method. The amount of TNF-α as an index of acute inflammatory except the 3rd day significantly decreased on the other day of reperfusion (7th, 147th and 287th days). The mast cells infiltration was significantly decreased on 77th and 147th days. The tissue edema was decreased significantly in honey administrated group in the comparison with placebo groups. Honey administration can reduce damage caused by ischemia-reperfusion in the rat gastrocnemius muscle.
author	Gholami,Mohammad Reza Abbaszadeh,Abolfazl Anbari,Khatereh Khaksarian,Mojtaba Shabooni,Fatemeh Khayat,Zahra Khanipour Khorramabadi,Reza Mohammadrezaei Gharravi,Anneh Mohammad
author_facet	Gholami,Mohammad Reza Abbaszadeh,Abolfazl Anbari,Khatereh Khaksarian,Mojtaba Shabooni,Fatemeh Khayat,Zahra Khanipour Khorramabadi,Reza Mohammadrezaei Gharravi,Anneh Mohammad
author_sort	Gholami,Mohammad Reza
title	Protective Effects of Honey, Apis mellifera Meda Skorikov, on Ischemia-Reperfusion Induced Muscle Injury
title_short	Protective Effects of Honey, Apis mellifera Meda Skorikov, on Ischemia-Reperfusion Induced Muscle Injury
title_full	Protective Effects of Honey, Apis mellifera Meda Skorikov, on Ischemia-Reperfusion Induced Muscle Injury
title_fullStr	Protective Effects of Honey, Apis mellifera Meda Skorikov, on Ischemia-Reperfusion Induced Muscle Injury
title_full_unstemmed	Protective Effects of Honey, Apis mellifera Meda Skorikov, on Ischemia-Reperfusion Induced Muscle Injury
title_sort	protective effects of honey, apis mellifera meda skorikov, on ischemia-reperfusion induced muscle injury
publisher	Sociedad Chilena de Anatomía
publishDate	2020
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022020000300804
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Protective Effects of Honey, Apis mellifera Meda Skorikov, on Ischemia-Reperfusion Induced Muscle Injury

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