A Histological and Histochemical Study on the Gallbladder of the Alburnus tarichi (Güldenstädt, 1814) (Cyprinidae)

SUMMARY: In the current study, the histological structure of the gallbladder of Alburnus tarichi (Güldenstädt, 1814) was investigated. Hematoxylin and eosin were used to stain the histological sections for routine examinations, in addition to using periodic acid Schiff (PAS) for the neutral mucins,...

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Autores principales:	Kaptaner,Burak, Aykut,Handan, Dogan,Emine
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2020
Materias:	Gallbladder Alburnus tarichi Fish Histology Histochemistry PCNA
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022020000400869
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spelling	oai:scielo:S0717-950220200004008692020-09-08A Histological and Histochemical Study on the Gallbladder of the Alburnus tarichi (Güldenstädt, 1814) (Cyprinidae)Kaptaner,BurakAykut,HandanDogan,Emine Gallbladder Alburnus tarichi Fish Histology Histochemistry PCNA SUMMARY: In the current study, the histological structure of the gallbladder of Alburnus tarichi (Güldenstädt, 1814) was investigated. Hematoxylin and eosin were used to stain the histological sections for routine examinations, in addition to using periodic acid Schiff (PAS) for the neutral mucins, aldehyde fuchsin (AF) for the sulphated mucins, and Alcian blue (AB; pH: 2.5) for the acidic mucins. In addition, proliferating cell nuclear antigen (PCNA) immune-staining was performed for the detection of dividing cells among the epithelium. The gallbladder of A. tarichi was composed of mucosa, muscularis, and serosa or adventitia layers. The mucosa covering the wavy pleomorphic folds was made up of tall columnar epithelium and a lamina propria. The apical surface of the epithelial cells was lined by continuous short microvilli. On the epithelium, the luminal surface was remarkably stained with PAS, AF, and AB. Slight to moderate staining was observed on the epithelial cells in the apical zone with PAS. The cytoplasm of epithelial cells were stained in a slight manner with AF. No goblet cells were observed among the epithelium. According to the PCNA immune-staining, some epithelial cells were observed to proliferate. The lamina propria consisted of loose connective tissue that contained fibrocytes, collagen and elastic fibers, capillaries, and small blood vessels. The muscularis layer displayed muscle fibers that were circular, smooth, and surrounded by collagen fibers. The subserosal and serosal or adventitial layers had typical morphology to those of other fish and vertebrates.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.38 n.4 20202020-08-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022020000400869en10.4067/S0717-95022020000400869
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Gallbladder Alburnus tarichi Fish Histology Histochemistry PCNA
spellingShingle	Gallbladder Alburnus tarichi Fish Histology Histochemistry PCNA Kaptaner,Burak Aykut,Handan Dogan,Emine A Histological and Histochemical Study on the Gallbladder of the Alburnus tarichi (Güldenstädt, 1814) (Cyprinidae)
description	SUMMARY: In the current study, the histological structure of the gallbladder of Alburnus tarichi (Güldenstädt, 1814) was investigated. Hematoxylin and eosin were used to stain the histological sections for routine examinations, in addition to using periodic acid Schiff (PAS) for the neutral mucins, aldehyde fuchsin (AF) for the sulphated mucins, and Alcian blue (AB; pH: 2.5) for the acidic mucins. In addition, proliferating cell nuclear antigen (PCNA) immune-staining was performed for the detection of dividing cells among the epithelium. The gallbladder of A. tarichi was composed of mucosa, muscularis, and serosa or adventitia layers. The mucosa covering the wavy pleomorphic folds was made up of tall columnar epithelium and a lamina propria. The apical surface of the epithelial cells was lined by continuous short microvilli. On the epithelium, the luminal surface was remarkably stained with PAS, AF, and AB. Slight to moderate staining was observed on the epithelial cells in the apical zone with PAS. The cytoplasm of epithelial cells were stained in a slight manner with AF. No goblet cells were observed among the epithelium. According to the PCNA immune-staining, some epithelial cells were observed to proliferate. The lamina propria consisted of loose connective tissue that contained fibrocytes, collagen and elastic fibers, capillaries, and small blood vessels. The muscularis layer displayed muscle fibers that were circular, smooth, and surrounded by collagen fibers. The subserosal and serosal or adventitial layers had typical morphology to those of other fish and vertebrates.
author	Kaptaner,Burak Aykut,Handan Dogan,Emine
author_facet	Kaptaner,Burak Aykut,Handan Dogan,Emine
author_sort	Kaptaner,Burak
title	A Histological and Histochemical Study on the Gallbladder of the Alburnus tarichi (Güldenstädt, 1814) (Cyprinidae)
title_short	A Histological and Histochemical Study on the Gallbladder of the Alburnus tarichi (Güldenstädt, 1814) (Cyprinidae)
title_full	A Histological and Histochemical Study on the Gallbladder of the Alburnus tarichi (Güldenstädt, 1814) (Cyprinidae)
title_fullStr	A Histological and Histochemical Study on the Gallbladder of the Alburnus tarichi (Güldenstädt, 1814) (Cyprinidae)
title_full_unstemmed	A Histological and Histochemical Study on the Gallbladder of the Alburnus tarichi (Güldenstädt, 1814) (Cyprinidae)
title_sort	histological and histochemical study on the gallbladder of the alburnus tarichi (güldenstädt, 1814) (cyprinidae)
publisher	Sociedad Chilena de Anatomía
publishDate	2020
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022020000400869
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A Histological and Histochemical Study on the Gallbladder of the Alburnus tarichi (Güldenstädt, 1814) (Cyprinidae)

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