Preventive Role of Gum Arabic Administration on STZ Induced Diabetic Kidney Disease in Rats; Renal Antioxidant and Histopathological Evidence

SUMMARY: This study was set to investigate the effect of gum Arabic (G.A.) on diabetic kidney disease. We divided sixty male Sprague rats randomly into six groups. Normal control, normal rats treated with G.A., untreated diabetic rats, diabetic rats treated with insulin, diabetic rats treated with G...

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Autores principales:	Muataz-E,Mohammed, Rehab-M,Badi, Osama-M,Osman, Mohamed-D,Morsy, Amr-M,Abbas, Salah-Omer,Bashir, Amal-M,Saeed
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2020
Materias:	Diabetes mellitus Nephropathy Gum Arabic Oxidative stress
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022020000401003
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spelling	oai:scielo:S0717-950220200004010032020-09-08Preventive Role of Gum Arabic Administration on STZ Induced Diabetic Kidney Disease in Rats; Renal Antioxidant and Histopathological EvidenceMuataz-E,MohammedRehab-M,BadiOsama-M,OsmanMohamed-D,MorsyAmr-M,AbbasSalah-Omer,BashirAmal-M,Saeed Diabetes mellitus Nephropathy Gum Arabic Oxidative stress SUMMARY: This study was set to investigate the effect of gum Arabic (G.A.) on diabetic kidney disease. We divided sixty male Sprague rats randomly into six groups. Normal control, normal rats treated with G.A., untreated diabetic rats, diabetic rats treated with insulin, diabetic rats treated with G.A., and diabetic rats treated with both insulin and G.A. Diabetes was induced by a single intraperitoneal injection of STZ. Forty eight hr post injections. Insulin was injected subcutaneously (1.6/IU/100g/day). We provided G.A. in drinking water (10 %w/ v).). At the end of the twelve weeks, blood was drawn for measurement of blood glucose, glycosylated hemoglobin (HbA1C), serum lipids, serum creatinine, and blood urea. Renal tissue oxidative stress (O.S.) was assessed by measuring the activities of superoxide dismutase (SOD) and catalase (CAT), and the concentrations of reduced glutathione (GSH) and malondialdehyde (MDA). For histological assessments, sections from segments of kidneys were processed and stained with hematoxylin and eosin (H&E) for assessment under the light microscope. STZinduced diabetes caused an elevation of blood glucose, HbA1c, urea and creatinine, triglycerides LDL and cholesterol, MDA with reduction of HDL, GSH level, and CAT and SOD activities. Histologically, kidneys from diabetic rats showed marked glomerular and tubular changes. Administration of G.A. alone to diabetic rats had a significant hypoglycemic, hypolipidemic, and antioxidant effect, although the levels achieved remained significantly abnormal compared with the untreated group with no effect on urea and creatinine levels. Co-administration of G.A. with insulin reversed the impact of D.M. on all parameters evaluated including the histological changes and led to normal urea and creatinine levels. We concluded that G.A., in combination with insulin, improves chemically-induced diabetes and its renal complications, possibly by modulation of oxidative stress.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.38 n.4 20202020-08-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022020000401003en10.4067/S0717-95022020000401003
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Diabetes mellitus Nephropathy Gum Arabic Oxidative stress
spellingShingle	Diabetes mellitus Nephropathy Gum Arabic Oxidative stress Muataz-E,Mohammed Rehab-M,Badi Osama-M,Osman Mohamed-D,Morsy Amr-M,Abbas Salah-Omer,Bashir Amal-M,Saeed Preventive Role of Gum Arabic Administration on STZ Induced Diabetic Kidney Disease in Rats; Renal Antioxidant and Histopathological Evidence
description	SUMMARY: This study was set to investigate the effect of gum Arabic (G.A.) on diabetic kidney disease. We divided sixty male Sprague rats randomly into six groups. Normal control, normal rats treated with G.A., untreated diabetic rats, diabetic rats treated with insulin, diabetic rats treated with G.A., and diabetic rats treated with both insulin and G.A. Diabetes was induced by a single intraperitoneal injection of STZ. Forty eight hr post injections. Insulin was injected subcutaneously (1.6/IU/100g/day). We provided G.A. in drinking water (10 %w/ v).). At the end of the twelve weeks, blood was drawn for measurement of blood glucose, glycosylated hemoglobin (HbA1C), serum lipids, serum creatinine, and blood urea. Renal tissue oxidative stress (O.S.) was assessed by measuring the activities of superoxide dismutase (SOD) and catalase (CAT), and the concentrations of reduced glutathione (GSH) and malondialdehyde (MDA). For histological assessments, sections from segments of kidneys were processed and stained with hematoxylin and eosin (H&E) for assessment under the light microscope. STZinduced diabetes caused an elevation of blood glucose, HbA1c, urea and creatinine, triglycerides LDL and cholesterol, MDA with reduction of HDL, GSH level, and CAT and SOD activities. Histologically, kidneys from diabetic rats showed marked glomerular and tubular changes. Administration of G.A. alone to diabetic rats had a significant hypoglycemic, hypolipidemic, and antioxidant effect, although the levels achieved remained significantly abnormal compared with the untreated group with no effect on urea and creatinine levels. Co-administration of G.A. with insulin reversed the impact of D.M. on all parameters evaluated including the histological changes and led to normal urea and creatinine levels. We concluded that G.A., in combination with insulin, improves chemically-induced diabetes and its renal complications, possibly by modulation of oxidative stress.
author	Muataz-E,Mohammed Rehab-M,Badi Osama-M,Osman Mohamed-D,Morsy Amr-M,Abbas Salah-Omer,Bashir Amal-M,Saeed
author_facet	Muataz-E,Mohammed Rehab-M,Badi Osama-M,Osman Mohamed-D,Morsy Amr-M,Abbas Salah-Omer,Bashir Amal-M,Saeed
author_sort	Muataz-E,Mohammed
title	Preventive Role of Gum Arabic Administration on STZ Induced Diabetic Kidney Disease in Rats; Renal Antioxidant and Histopathological Evidence
title_short	Preventive Role of Gum Arabic Administration on STZ Induced Diabetic Kidney Disease in Rats; Renal Antioxidant and Histopathological Evidence
title_full	Preventive Role of Gum Arabic Administration on STZ Induced Diabetic Kidney Disease in Rats; Renal Antioxidant and Histopathological Evidence
title_fullStr	Preventive Role of Gum Arabic Administration on STZ Induced Diabetic Kidney Disease in Rats; Renal Antioxidant and Histopathological Evidence
title_full_unstemmed	Preventive Role of Gum Arabic Administration on STZ Induced Diabetic Kidney Disease in Rats; Renal Antioxidant and Histopathological Evidence
title_sort	preventive role of gum arabic administration on stz induced diabetic kidney disease in rats; renal antioxidant and histopathological evidence
publisher	Sociedad Chilena de Anatomía
publishDate	2020
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022020000401003
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Preventive Role of Gum Arabic Administration on STZ Induced Diabetic Kidney Disease in Rats; Renal Antioxidant and Histopathological Evidence

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