Effects of Chronic Stretching on Soleus Muscle of Wistar Female Rats

SUMMARY: The purpose of this study was to evaluate the chronic effects of stretching exercise on soleus muscle histomorphology and histomorphometry of young and aged rats. Thirty-eight female rats were divided into young control group (YCG, n=10;274±50 g); young stretching group (YSG, n=8;274±12 g);...

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Autores principales:	Massenz,Kátia Janine Veiga, Zotz,Talita Gianello Gnoato, Martins,Hilana Rickli Fiuza, Campos,Talita Mirele, Bertoncini,Nayara, Capriglione,Luiz Guilherme A, Noronha,Lúcia, Azevedo,Marina Luise Viola De, Gomes,Anna Raquel Silveira
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2020
Materias:	Aging Muscle stretching exercise Cellular mechanotransduction Muscle plasticity
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022020000401039
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spelling	oai:scielo:S0717-950220200004010392020-09-08Effects of Chronic Stretching on Soleus Muscle of Wistar Female RatsMassenz,Kátia Janine VeigaZotz,Talita Gianello GnoatoMartins,Hilana Rickli FiuzaCampos,Talita MireleBertoncini,NayaraCapriglione,Luiz Guilherme ANoronha,LúciaAzevedo,Marina Luise Viola DeGomes,Anna Raquel Silveira Aging Muscle stretching exercise Cellular mechanotransduction Muscle plasticity SUMMARY: The purpose of this study was to evaluate the chronic effects of stretching exercise on soleus muscle histomorphology and histomorphometry of young and aged rats. Thirty-eight female rats were divided into young control group (YCG, n=10;274±50 g); young stretching group (YSG, n=8;274±12 g); aged control group (ACG, n=10;335±39 g); and aged stretching group (ASG, n=10;321±32g). A mechanical apparatus was used to stretch muscle in 4 repetitions, 60 s each, 30 s interval between repetitions in each session, 3 times a week for 3 weeks. Twenty-four hours after the last stretching session, soleus muscle was removed for micromorphology and immunostaining analysis. Data analyses were performed with one-way ANOVA, post-hoc Tukey, or Kruskal-Wallis tests for parametric and nonparametric, respectively (p≤0.05). Muscle fiber cross-sectional area (MFCSA) of ACG was lower (18 %) compared to the YCG. Stretching increased MFCSA comparing YSG to YCG (5,681.15± 1,943.61 µm2 vs 5,119.84±1,857.73 µm2, p=0.00), but decreased comparing ASG to ACG (3,919.54± 1,694.65 µm2 vs 4,172.82±1,446.08 µm2, p=0.00). More serial sarcomere numbers were found in the YSG than YCG (12,062.91±1,564.68 vs 10,070.39±1,072.38, p=0.03). Collagen I and collagen III were higher in YSG than ASG (7.44±7.18 % vs 0.07±0.09 %, p=0.04) and (14.37 %± 9.54 % vs 5.51 %±5.52 %, p=0.00), respectively. TNF-a was greater in ASG than YSG (43.42 %±40.19 % vs 1.72 ± 2.02 %, p=0.00). Epimysium was larger in the YSG compared to YCG (201.83±132.07 % vs 181.09±147.04 %, p=0.00). After 3-week stretching the soleus muscles from aged rats were smaller than their younger counter-parts. Interestingly, while stretching appeared to positively affect young soleus muscle, the opposite was detected in the muscle of the aged rats.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.38 n.4 20202020-08-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022020000401039en10.4067/S0717-95022020000401039
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Aging Muscle stretching exercise Cellular mechanotransduction Muscle plasticity
spellingShingle	Aging Muscle stretching exercise Cellular mechanotransduction Muscle plasticity Massenz,Kátia Janine Veiga Zotz,Talita Gianello Gnoato Martins,Hilana Rickli Fiuza Campos,Talita Mirele Bertoncini,Nayara Capriglione,Luiz Guilherme A Noronha,Lúcia Azevedo,Marina Luise Viola De Gomes,Anna Raquel Silveira Effects of Chronic Stretching on Soleus Muscle of Wistar Female Rats
description	SUMMARY: The purpose of this study was to evaluate the chronic effects of stretching exercise on soleus muscle histomorphology and histomorphometry of young and aged rats. Thirty-eight female rats were divided into young control group (YCG, n=10;274±50 g); young stretching group (YSG, n=8;274±12 g); aged control group (ACG, n=10;335±39 g); and aged stretching group (ASG, n=10;321±32g). A mechanical apparatus was used to stretch muscle in 4 repetitions, 60 s each, 30 s interval between repetitions in each session, 3 times a week for 3 weeks. Twenty-four hours after the last stretching session, soleus muscle was removed for micromorphology and immunostaining analysis. Data analyses were performed with one-way ANOVA, post-hoc Tukey, or Kruskal-Wallis tests for parametric and nonparametric, respectively (p≤0.05). Muscle fiber cross-sectional area (MFCSA) of ACG was lower (18 %) compared to the YCG. Stretching increased MFCSA comparing YSG to YCG (5,681.15± 1,943.61 µm2 vs 5,119.84±1,857.73 µm2, p=0.00), but decreased comparing ASG to ACG (3,919.54± 1,694.65 µm2 vs 4,172.82±1,446.08 µm2, p=0.00). More serial sarcomere numbers were found in the YSG than YCG (12,062.91±1,564.68 vs 10,070.39±1,072.38, p=0.03). Collagen I and collagen III were higher in YSG than ASG (7.44±7.18 % vs 0.07±0.09 %, p=0.04) and (14.37 %± 9.54 % vs 5.51 %±5.52 %, p=0.00), respectively. TNF-a was greater in ASG than YSG (43.42 %±40.19 % vs 1.72 ± 2.02 %, p=0.00). Epimysium was larger in the YSG compared to YCG (201.83±132.07 % vs 181.09±147.04 %, p=0.00). After 3-week stretching the soleus muscles from aged rats were smaller than their younger counter-parts. Interestingly, while stretching appeared to positively affect young soleus muscle, the opposite was detected in the muscle of the aged rats.
author	Massenz,Kátia Janine Veiga Zotz,Talita Gianello Gnoato Martins,Hilana Rickli Fiuza Campos,Talita Mirele Bertoncini,Nayara Capriglione,Luiz Guilherme A Noronha,Lúcia Azevedo,Marina Luise Viola De Gomes,Anna Raquel Silveira
author_facet	Massenz,Kátia Janine Veiga Zotz,Talita Gianello Gnoato Martins,Hilana Rickli Fiuza Campos,Talita Mirele Bertoncini,Nayara Capriglione,Luiz Guilherme A Noronha,Lúcia Azevedo,Marina Luise Viola De Gomes,Anna Raquel Silveira
author_sort	Massenz,Kátia Janine Veiga
title	Effects of Chronic Stretching on Soleus Muscle of Wistar Female Rats
title_short	Effects of Chronic Stretching on Soleus Muscle of Wistar Female Rats
title_full	Effects of Chronic Stretching on Soleus Muscle of Wistar Female Rats
title_fullStr	Effects of Chronic Stretching on Soleus Muscle of Wistar Female Rats
title_full_unstemmed	Effects of Chronic Stretching on Soleus Muscle of Wistar Female Rats
title_sort	effects of chronic stretching on soleus muscle of wistar female rats
publisher	Sociedad Chilena de Anatomía
publishDate	2020
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022020000401039
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Effects of Chronic Stretching on Soleus Muscle of Wistar Female Rats

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