The Modified Method of Luxol Fast Blue for Paraffin-Embedded Myelin Sheath Staining

SUMMARY: Histological techniques are the study of animal and human tissues through staining and examining them under a microscope. To demonstrate the axonal degeneration and demyelination in histological studies, the Luxol Fast Blue staining is gold standard techniques. In this study, a new histoche...

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Autores principales:	Sajadi,Ensieh, Raoofi,Amir, Abdi,Shabnam, Azimi,Hadi, Abdollahifar,Mohammad-Amin
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2020
Materias:	Myelin sheath Luxol Fast Blue Light Microscope
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022020000501197
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spelling	oai:scielo:S0717-950220200005011972021-06-10The Modified Method of Luxol Fast Blue for Paraffin-Embedded Myelin Sheath StainingSajadi,EnsiehRaoofi,AmirAbdi,ShabnamAzimi,HadiAbdollahifar,Mohammad-Amin Myelin sheath Luxol Fast Blue Light Microscope SUMMARY: Histological techniques are the study of animal and human tissues through staining and examining them under a microscope. To demonstrate the axonal degeneration and demyelination in histological studies, the Luxol Fast Blue staining is gold standard techniques. In this study, a new histochemical method based on modified Luxol Fast Blue for the staining of the myelin sheath in sciatic nerve tissues described. The sciatic nerves of rats were removed and then the sciatic nerve was immersed in 10 % formaldehyde for one week and embedded in paraffin block. Next, thin sections (5 µm) were cut, using a microtome and stained with conventional and modified Luxol Fast Blue. Our results showed that a new method of modified Luxol Fast Blue staining can accurately identify the myelin in the sciatic nerve fibers. The current study showed that the Luxol Fast Blue combination with Light Green has a good effect on myelin coloration, and the results of this study are comparable to LFB combination with Sirius red.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.38 n.5 20202020-10-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022020000501197en10.4067/S0717-95022020000501197
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Myelin sheath Luxol Fast Blue Light Microscope
spellingShingle	Myelin sheath Luxol Fast Blue Light Microscope Sajadi,Ensieh Raoofi,Amir Abdi,Shabnam Azimi,Hadi Abdollahifar,Mohammad-Amin The Modified Method of Luxol Fast Blue for Paraffin-Embedded Myelin Sheath Staining
description	SUMMARY: Histological techniques are the study of animal and human tissues through staining and examining them under a microscope. To demonstrate the axonal degeneration and demyelination in histological studies, the Luxol Fast Blue staining is gold standard techniques. In this study, a new histochemical method based on modified Luxol Fast Blue for the staining of the myelin sheath in sciatic nerve tissues described. The sciatic nerves of rats were removed and then the sciatic nerve was immersed in 10 % formaldehyde for one week and embedded in paraffin block. Next, thin sections (5 µm) were cut, using a microtome and stained with conventional and modified Luxol Fast Blue. Our results showed that a new method of modified Luxol Fast Blue staining can accurately identify the myelin in the sciatic nerve fibers. The current study showed that the Luxol Fast Blue combination with Light Green has a good effect on myelin coloration, and the results of this study are comparable to LFB combination with Sirius red.
author	Sajadi,Ensieh Raoofi,Amir Abdi,Shabnam Azimi,Hadi Abdollahifar,Mohammad-Amin
author_facet	Sajadi,Ensieh Raoofi,Amir Abdi,Shabnam Azimi,Hadi Abdollahifar,Mohammad-Amin
author_sort	Sajadi,Ensieh
title	The Modified Method of Luxol Fast Blue for Paraffin-Embedded Myelin Sheath Staining
title_short	The Modified Method of Luxol Fast Blue for Paraffin-Embedded Myelin Sheath Staining
title_full	The Modified Method of Luxol Fast Blue for Paraffin-Embedded Myelin Sheath Staining
title_fullStr	The Modified Method of Luxol Fast Blue for Paraffin-Embedded Myelin Sheath Staining
title_full_unstemmed	The Modified Method of Luxol Fast Blue for Paraffin-Embedded Myelin Sheath Staining
title_sort	modified method of luxol fast blue for paraffin-embedded myelin sheath staining
publisher	Sociedad Chilena de Anatomía
publishDate	2020
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022020000501197
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The Modified Method of Luxol Fast Blue for Paraffin-Embedded Myelin Sheath Staining

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