Qualitative and Quantitative Ultrastructural Analysis of the Mitochondria from Adrenal Gland Cortex Under the Action of Viperidae family Snake Venoms

SUMMARY: The Viperidae venoms are composed of a mixture of constituents with enzymatic and non-enzymatic actions, which act on ultrastructural components of cells and tissues. Here, the number of mitochondria, mitochondrial area and the number of mitochondrial cristae from adrenal glands cortex trea...

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Autores principales:	Finol,Héctor J, García,Estefanie, González,Roschman, Sanchez,Elda E, Rodríguez-Acosta,Alexis
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2020
Materias:	Adrenal gland Bothrops venezuelensis Cristae Crotalus pifanorum Crotalus vegrandis Mitochondria Venom
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022020000501271
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spelling	oai:scielo:S0717-950220200005012712021-06-10Qualitative and Quantitative Ultrastructural Analysis of the Mitochondria from Adrenal Gland Cortex Under the Action of Viperidae family Snake VenomsFinol,Héctor JGarcía,EstefanieGonzález,RoschmanSanchez,Elda ERodríguez-Acosta,Alexis Adrenal gland Bothrops venezuelensis Cristae Crotalus pifanorum Crotalus vegrandis Mitochondria Venom SUMMARY: The Viperidae venoms are composed of a mixture of constituents with enzymatic and non-enzymatic actions, which act on ultrastructural components of cells and tissues. Here, the number of mitochondria, mitochondrial area and the number of mitochondrial cristae from adrenal glands cortex treated with snake venoms were tested after 3, 6 and 24 hours of venom injections. The mitochondria quantitative changes showed a statistically significant decrease, in the number of mitochondria past 3, 6 and 24 h. There was an increase in the mitochondrial area after 6 h, where Crotalus vegrandis venom did not present significant differences with Crotalus pifanorum or Bothrops venezuelensis venoms. After 24 h, there was an escalation of mitochondrial area in all tested venoms. The number of mitochondrial cristae after 3 h did not present important differences with the control treatment. After 6 h, the number of mitochondrial cristae initiated to decrease under the activities of the 3 venoms action, until 24 h of observation. In the qualitative observations it was possible to witness an intense damage of the mitochondria, with loss and swelling of membranes, disappearance of cristae and the appearance of myelin figures, which started at 3 h after the Crotalus and Bothrops venoms injections. These damages probably were due to cytotoxic effects of phospholipases, metalloproteases and/or other proteolytic activities present in Viperidae snake venoms, being more evident in Crotalus venoms. As far as we know, these results define a novel finding that suggest that Viperidae snake venoms are extremely toxic to mammalian mitochondria.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.38 n.5 20202020-10-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022020000501271en10.4067/S0717-95022020000501271
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Adrenal gland Bothrops venezuelensis Cristae Crotalus pifanorum Crotalus vegrandis Mitochondria Venom
spellingShingle	Adrenal gland Bothrops venezuelensis Cristae Crotalus pifanorum Crotalus vegrandis Mitochondria Venom Finol,Héctor J García,Estefanie González,Roschman Sanchez,Elda E Rodríguez-Acosta,Alexis Qualitative and Quantitative Ultrastructural Analysis of the Mitochondria from Adrenal Gland Cortex Under the Action of Viperidae family Snake Venoms
description	SUMMARY: The Viperidae venoms are composed of a mixture of constituents with enzymatic and non-enzymatic actions, which act on ultrastructural components of cells and tissues. Here, the number of mitochondria, mitochondrial area and the number of mitochondrial cristae from adrenal glands cortex treated with snake venoms were tested after 3, 6 and 24 hours of venom injections. The mitochondria quantitative changes showed a statistically significant decrease, in the number of mitochondria past 3, 6 and 24 h. There was an increase in the mitochondrial area after 6 h, where Crotalus vegrandis venom did not present significant differences with Crotalus pifanorum or Bothrops venezuelensis venoms. After 24 h, there was an escalation of mitochondrial area in all tested venoms. The number of mitochondrial cristae after 3 h did not present important differences with the control treatment. After 6 h, the number of mitochondrial cristae initiated to decrease under the activities of the 3 venoms action, until 24 h of observation. In the qualitative observations it was possible to witness an intense damage of the mitochondria, with loss and swelling of membranes, disappearance of cristae and the appearance of myelin figures, which started at 3 h after the Crotalus and Bothrops venoms injections. These damages probably were due to cytotoxic effects of phospholipases, metalloproteases and/or other proteolytic activities present in Viperidae snake venoms, being more evident in Crotalus venoms. As far as we know, these results define a novel finding that suggest that Viperidae snake venoms are extremely toxic to mammalian mitochondria.
author	Finol,Héctor J García,Estefanie González,Roschman Sanchez,Elda E Rodríguez-Acosta,Alexis
author_facet	Finol,Héctor J García,Estefanie González,Roschman Sanchez,Elda E Rodríguez-Acosta,Alexis
author_sort	Finol,Héctor J
title	Qualitative and Quantitative Ultrastructural Analysis of the Mitochondria from Adrenal Gland Cortex Under the Action of Viperidae family Snake Venoms
title_short	Qualitative and Quantitative Ultrastructural Analysis of the Mitochondria from Adrenal Gland Cortex Under the Action of Viperidae family Snake Venoms
title_full	Qualitative and Quantitative Ultrastructural Analysis of the Mitochondria from Adrenal Gland Cortex Under the Action of Viperidae family Snake Venoms
title_fullStr	Qualitative and Quantitative Ultrastructural Analysis of the Mitochondria from Adrenal Gland Cortex Under the Action of Viperidae family Snake Venoms
title_full_unstemmed	Qualitative and Quantitative Ultrastructural Analysis of the Mitochondria from Adrenal Gland Cortex Under the Action of Viperidae family Snake Venoms
title_sort	qualitative and quantitative ultrastructural analysis of the mitochondria from adrenal gland cortex under the action of viperidae family snake venoms
publisher	Sociedad Chilena de Anatomía
publishDate	2020
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022020000501271
work_keys_str_mv	AT finolhectorj qualitativeandquantitativeultrastructuralanalysisofthemitochondriafromadrenalglandcortexundertheactionofviperidaefamilysnakevenoms AT garciaestefanie qualitativeandquantitativeultrastructuralanalysisofthemitochondriafromadrenalglandcortexundertheactionofviperidaefamilysnakevenoms AT gonzalezroschman qualitativeandquantitativeultrastructuralanalysisofthemitochondriafromadrenalglandcortexundertheactionofviperidaefamilysnakevenoms AT sanchezeldae qualitativeandquantitativeultrastructuralanalysisofthemitochondriafromadrenalglandcortexundertheactionofviperidaefamilysnakevenoms AT rodriguezacostaalexis qualitativeandquantitativeultrastructuralanalysisofthemitochondriafromadrenalglandcortexundertheactionofviperidaefamilysnakevenoms
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Qualitative and Quantitative Ultrastructural Analysis of the Mitochondria from Adrenal Gland Cortex Under the Action of Viperidae family Snake Venoms

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