Effects of Ultrasound and Stretching on Skeletal Muscle Contusion in Rats: Immunohistochemistry Analysis

SUMMARY: The aim of this study was to evaluate the effects of stretching and therapeutic ultrasound (TUS) on desmin and laminin contents of rat muscle after contusion. Male Wistar rats (n = 35, 8-9 weeks of age, 271 ± 14g body weight) were divided into five groups: Control group (CG) (n= 03); Injure...

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Autores principales:	de-Macedo,Ana Carolina Brandt, Ywazaki,Julye Leiko, Martins,Ana Paula Camargo, Azevedo,Marina Luise Viola de, Noronha,Lucia, Gomes,Anna Raquel Silveira
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2020
Materias:	Muscle stretching exercises Ultrasonic therapy Desmin Laminin rats
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022020000501288
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spelling	oai:scielo:S0717-950220200005012882021-06-10Effects of Ultrasound and Stretching on Skeletal Muscle Contusion in Rats: Immunohistochemistry Analysisde-Macedo,Ana Carolina BrandtYwazaki,Julye LeikoMartins,Ana Paula CamargoAzevedo,Marina Luise Viola deNoronha,LuciaGomes,Anna Raquel Silveira Muscle stretching exercises Ultrasonic therapy Desmin Laminin rats SUMMARY: The aim of this study was to evaluate the effects of stretching and therapeutic ultrasound (TUS) on desmin and laminin contents of rat muscle after contusion. Male Wistar rats (n = 35, 8-9 weeks of age, 271 ± 14g body weight) were divided into five groups: Control group (CG) (n= 03); Injured group (IG) (n= 8); Injured + ultrasound group (IUSG) (n= 8); Injured+stretching group (ISG) (n= 8); Injured +ultrasound + stretching group (IUSSG) (n= 8). The application of ultrasound started 72 hours after the contusion, using the 50 % pulsed mode, 0.5 W/cm2, 5 min, once a day, for five consecutive days. Passive manual stretching was started on the tenth day after injury, with four repetitions of 30 s each and 30 s rest between repetitions, once a day, five times per week, for a total of ten applications. After 22 days, the rats were euthanazied and the gastrocnemius of both limbs removed for desmin and laminin immunohistochemistry morphometric measurement. Analysis was conducted using ANOVA one way post-hoc Tukey to parametric data and Kruskall-Wallis for non-parametric data. The IUSSG animals showed a larger area of desmin than ISG (p<0.05). It was found a decrease in laminin comparing IUSG to IG. However, laminin area was higher in ISG than all groups (p<0.05). UST isolated or in combination with stretching influenced gastrocnemius regeneration in different manners. While stretching applied isolated enhanced gastrocnemius regeneration noticed by the increase in laminin area, in combination with TUS strengthened the muscle healing rising desmin area.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.38 n.5 20202020-10-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022020000501288en10.4067/S0717-95022020000501288
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Muscle stretching exercises Ultrasonic therapy Desmin Laminin rats
spellingShingle	Muscle stretching exercises Ultrasonic therapy Desmin Laminin rats de-Macedo,Ana Carolina Brandt Ywazaki,Julye Leiko Martins,Ana Paula Camargo Azevedo,Marina Luise Viola de Noronha,Lucia Gomes,Anna Raquel Silveira Effects of Ultrasound and Stretching on Skeletal Muscle Contusion in Rats: Immunohistochemistry Analysis
description	SUMMARY: The aim of this study was to evaluate the effects of stretching and therapeutic ultrasound (TUS) on desmin and laminin contents of rat muscle after contusion. Male Wistar rats (n = 35, 8-9 weeks of age, 271 ± 14g body weight) were divided into five groups: Control group (CG) (n= 03); Injured group (IG) (n= 8); Injured + ultrasound group (IUSG) (n= 8); Injured+stretching group (ISG) (n= 8); Injured +ultrasound + stretching group (IUSSG) (n= 8). The application of ultrasound started 72 hours after the contusion, using the 50 % pulsed mode, 0.5 W/cm2, 5 min, once a day, for five consecutive days. Passive manual stretching was started on the tenth day after injury, with four repetitions of 30 s each and 30 s rest between repetitions, once a day, five times per week, for a total of ten applications. After 22 days, the rats were euthanazied and the gastrocnemius of both limbs removed for desmin and laminin immunohistochemistry morphometric measurement. Analysis was conducted using ANOVA one way post-hoc Tukey to parametric data and Kruskall-Wallis for non-parametric data. The IUSSG animals showed a larger area of desmin than ISG (p<0.05). It was found a decrease in laminin comparing IUSG to IG. However, laminin area was higher in ISG than all groups (p<0.05). UST isolated or in combination with stretching influenced gastrocnemius regeneration in different manners. While stretching applied isolated enhanced gastrocnemius regeneration noticed by the increase in laminin area, in combination with TUS strengthened the muscle healing rising desmin area.
author	de-Macedo,Ana Carolina Brandt Ywazaki,Julye Leiko Martins,Ana Paula Camargo Azevedo,Marina Luise Viola de Noronha,Lucia Gomes,Anna Raquel Silveira
author_facet	de-Macedo,Ana Carolina Brandt Ywazaki,Julye Leiko Martins,Ana Paula Camargo Azevedo,Marina Luise Viola de Noronha,Lucia Gomes,Anna Raquel Silveira
author_sort	de-Macedo,Ana Carolina Brandt
title	Effects of Ultrasound and Stretching on Skeletal Muscle Contusion in Rats: Immunohistochemistry Analysis
title_short	Effects of Ultrasound and Stretching on Skeletal Muscle Contusion in Rats: Immunohistochemistry Analysis
title_full	Effects of Ultrasound and Stretching on Skeletal Muscle Contusion in Rats: Immunohistochemistry Analysis
title_fullStr	Effects of Ultrasound and Stretching on Skeletal Muscle Contusion in Rats: Immunohistochemistry Analysis
title_full_unstemmed	Effects of Ultrasound and Stretching on Skeletal Muscle Contusion in Rats: Immunohistochemistry Analysis
title_sort	effects of ultrasound and stretching on skeletal muscle contusion in rats: immunohistochemistry analysis
publisher	Sociedad Chilena de Anatomía
publishDate	2020
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022020000501288
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Effects of Ultrasound and Stretching on Skeletal Muscle Contusion in Rats: Immunohistochemistry Analysis

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