Effects of Corchorus olitorius and Protacatechuic Acid on Diabetic Rat Testis Tissue

SUMMARY: The aim of this study is to investigate the effects of Protocatechuic acid and Corchorus olitorius on streptozotocin (STZ) induced diabetic rat testis tissue. Randomly selected Wistar Albino rats were divided into five groups as; Diabetes Mellitus, Diabetes Mellitus treated with Corchorus O...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Mercan,Nedime, Toros,Pelin, Söyler,Gizem, Hanoglu,Azmi, Kükner,Aysel
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2020
Materias:	Streptozotosin Diabetus mellitus Testis Corchorus olitorius Protocatechuic acid
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022020000501330
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0717-95022020000501330
record_format	dspace
spelling	oai:scielo:S0717-950220200005013302021-06-10Effects of Corchorus olitorius and Protacatechuic Acid on Diabetic Rat Testis TissueMercan,NedimeToros,PelinSöyler,GizemHanoglu,AzmiKükner,Aysel Streptozotosin Diabetus mellitus Testis Corchorus olitorius Protocatechuic acid SUMMARY: The aim of this study is to investigate the effects of Protocatechuic acid and Corchorus olitorius on streptozotocin (STZ) induced diabetic rat testis tissue. Randomly selected Wistar Albino rats were divided into five groups as; Diabetes Mellitus, Diabetes Mellitus treated with Corchorus Olitorus (STZ+CO), Diabetes Mellitus treated with Protacatechuic acid (STZ+PCA), Corchorus olitorius (CO), Protocatechuic acid (PCA) and Control. Diabetic model was generated by intraperitoneal injection of 60 mg/kg Streptozotosin. After 48 hours of the STZ injection, blood samples were collected from tail vein in order to measure blood glycose levels. Over 250 mg/dL accepted as diabetic subjets and fed with 250 mg/kg Corchorus olitorius or 20 mg/kg PCA by oral gavage for three weeks. At the end of the experiment, right testes were removed and fixed in 10 % neutral formaldehyde for paraffine embedding. Sections were stained by HE, Masson trichrome, PAS and TUNEL for microscopic evaluation. Control, PCA-only and Corchorus olitorius-only treated group testes tissues showed a normal tissue organization, when degeneration in seminiferous tubules, the vacuolization, seperations in spermatogenic cell series, outpouring of cell groups in the lumen, vesicular body formation, liquid accumulation in the interstitial region and edema were observed in STZ induced diabetic models and untreated groups. Besides, higher amount of TUNEL (+) stained cells were determined in STZ group. On the other hand, blood glucose level and number of TUNEL (+) stained cells were decreased as a result of PCA and Corchorus olitorius treatment. Because of the reduction of blood glucose level and apoptotic cell numbers, PCA and Corchorus olitorius decreace the complications of diabetes mellitus induced rat testis.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.38 n.5 20202020-10-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022020000501330en10.4067/S0717-95022020000501330
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Streptozotosin Diabetus mellitus Testis Corchorus olitorius Protocatechuic acid
spellingShingle	Streptozotosin Diabetus mellitus Testis Corchorus olitorius Protocatechuic acid Mercan,Nedime Toros,Pelin Söyler,Gizem Hanoglu,Azmi Kükner,Aysel Effects of Corchorus olitorius and Protacatechuic Acid on Diabetic Rat Testis Tissue
description	SUMMARY: The aim of this study is to investigate the effects of Protocatechuic acid and Corchorus olitorius on streptozotocin (STZ) induced diabetic rat testis tissue. Randomly selected Wistar Albino rats were divided into five groups as; Diabetes Mellitus, Diabetes Mellitus treated with Corchorus Olitorus (STZ+CO), Diabetes Mellitus treated with Protacatechuic acid (STZ+PCA), Corchorus olitorius (CO), Protocatechuic acid (PCA) and Control. Diabetic model was generated by intraperitoneal injection of 60 mg/kg Streptozotosin. After 48 hours of the STZ injection, blood samples were collected from tail vein in order to measure blood glycose levels. Over 250 mg/dL accepted as diabetic subjets and fed with 250 mg/kg Corchorus olitorius or 20 mg/kg PCA by oral gavage for three weeks. At the end of the experiment, right testes were removed and fixed in 10 % neutral formaldehyde for paraffine embedding. Sections were stained by HE, Masson trichrome, PAS and TUNEL for microscopic evaluation. Control, PCA-only and Corchorus olitorius-only treated group testes tissues showed a normal tissue organization, when degeneration in seminiferous tubules, the vacuolization, seperations in spermatogenic cell series, outpouring of cell groups in the lumen, vesicular body formation, liquid accumulation in the interstitial region and edema were observed in STZ induced diabetic models and untreated groups. Besides, higher amount of TUNEL (+) stained cells were determined in STZ group. On the other hand, blood glucose level and number of TUNEL (+) stained cells were decreased as a result of PCA and Corchorus olitorius treatment. Because of the reduction of blood glucose level and apoptotic cell numbers, PCA and Corchorus olitorius decreace the complications of diabetes mellitus induced rat testis.
author	Mercan,Nedime Toros,Pelin Söyler,Gizem Hanoglu,Azmi Kükner,Aysel
author_facet	Mercan,Nedime Toros,Pelin Söyler,Gizem Hanoglu,Azmi Kükner,Aysel
author_sort	Mercan,Nedime
title	Effects of Corchorus olitorius and Protacatechuic Acid on Diabetic Rat Testis Tissue
title_short	Effects of Corchorus olitorius and Protacatechuic Acid on Diabetic Rat Testis Tissue
title_full	Effects of Corchorus olitorius and Protacatechuic Acid on Diabetic Rat Testis Tissue
title_fullStr	Effects of Corchorus olitorius and Protacatechuic Acid on Diabetic Rat Testis Tissue
title_full_unstemmed	Effects of Corchorus olitorius and Protacatechuic Acid on Diabetic Rat Testis Tissue
title_sort	effects of corchorus olitorius and protacatechuic acid on diabetic rat testis tissue
publisher	Sociedad Chilena de Anatomía
publishDate	2020
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022020000501330
work_keys_str_mv	AT mercannedime effectsofcorchorusolitoriusandprotacatechuicacidondiabeticrattestistissue AT torospelin effectsofcorchorusolitoriusandprotacatechuicacidondiabeticrattestistissue AT soylergizem effectsofcorchorusolitoriusandprotacatechuicacidondiabeticrattestistissue AT hanogluazmi effectsofcorchorusolitoriusandprotacatechuicacidondiabeticrattestistissue AT kukneraysel effectsofcorchorusolitoriusandprotacatechuicacidondiabeticrattestistissue
_version_	1718445164493537280

Effects of Corchorus olitorius and Protacatechuic Acid on Diabetic Rat Testis Tissue

Ejemplares similares