Alcohol-Based Fixatives can Better Preserve Tissue Morphology than Formalin

SUMMARY: Fixation is a crucial step in processing of tissue specimen for preservation of cellular architecture and composition of cells. Alcohol-based fixatives are considered some of the most promising alternatives to formalin. We evaluated the performance of alcohol-based fixatives (EthMeth and me...

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Autores principales:	Haque,Ziaul, Rahman,Asabur, Khan,Zahirul Islam, Hussan,Mohammad Tufazzal, Alam,Mahmudul
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2020
Materias:	Fixation EthMeth Methacarn Formalin Tissue morphology
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022020000501371
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spelling	oai:scielo:S0717-950220200005013712021-06-10Alcohol-Based Fixatives can Better Preserve Tissue Morphology than FormalinHaque,ZiaulRahman,AsaburKhan,Zahirul IslamHussan,Mohammad TufazzalAlam,Mahmudul Fixation EthMeth Methacarn Formalin Tissue morphology SUMMARY: Fixation is a crucial step in processing of tissue specimen for preservation of cellular architecture and composition of cells. Alcohol-based fixatives are considered some of the most promising alternatives to formalin. We evaluated the performance of alcohol-based fixatives (EthMeth and methacarn) and formalin as a comparator fixative in the research laboratory. Following 24 hours of fixation, tissue morphology and cellular details of the liver, spleen and brain (cerebral cortex) were evaluated. Morphological characteristics were evaluated by gross observations and analyzing cellular details, tissue architecture and overall staining characteristics (Hematoxylin and Eosin). EthMeth and methacarn fixation gave generally comparable and satisfactory results on the tissue morphology and subsequent identification of tissue characteristics. Particularly, tissues were well preserved and all nuclear as well as cytoplasmic details were clearly visible. However, formalin fixed tissues showed some peculiarity such as improper fixation, mild shrinkage, and alterations of tissue components. These results confirm that alcohol-based fixation is the superior alternative to formalin for preservation of tissue morphology. However, it is required to standardize the formalin-free methods and harmonize diagnosis in the laboratory worldwide.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.38 n.5 20202020-10-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022020000501371en10.4067/S0717-95022020000501371
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Fixation EthMeth Methacarn Formalin Tissue morphology
spellingShingle	Fixation EthMeth Methacarn Formalin Tissue morphology Haque,Ziaul Rahman,Asabur Khan,Zahirul Islam Hussan,Mohammad Tufazzal Alam,Mahmudul Alcohol-Based Fixatives can Better Preserve Tissue Morphology than Formalin
description	SUMMARY: Fixation is a crucial step in processing of tissue specimen for preservation of cellular architecture and composition of cells. Alcohol-based fixatives are considered some of the most promising alternatives to formalin. We evaluated the performance of alcohol-based fixatives (EthMeth and methacarn) and formalin as a comparator fixative in the research laboratory. Following 24 hours of fixation, tissue morphology and cellular details of the liver, spleen and brain (cerebral cortex) were evaluated. Morphological characteristics were evaluated by gross observations and analyzing cellular details, tissue architecture and overall staining characteristics (Hematoxylin and Eosin). EthMeth and methacarn fixation gave generally comparable and satisfactory results on the tissue morphology and subsequent identification of tissue characteristics. Particularly, tissues were well preserved and all nuclear as well as cytoplasmic details were clearly visible. However, formalin fixed tissues showed some peculiarity such as improper fixation, mild shrinkage, and alterations of tissue components. These results confirm that alcohol-based fixation is the superior alternative to formalin for preservation of tissue morphology. However, it is required to standardize the formalin-free methods and harmonize diagnosis in the laboratory worldwide.
author	Haque,Ziaul Rahman,Asabur Khan,Zahirul Islam Hussan,Mohammad Tufazzal Alam,Mahmudul
author_facet	Haque,Ziaul Rahman,Asabur Khan,Zahirul Islam Hussan,Mohammad Tufazzal Alam,Mahmudul
author_sort	Haque,Ziaul
title	Alcohol-Based Fixatives can Better Preserve Tissue Morphology than Formalin
title_short	Alcohol-Based Fixatives can Better Preserve Tissue Morphology than Formalin
title_full	Alcohol-Based Fixatives can Better Preserve Tissue Morphology than Formalin
title_fullStr	Alcohol-Based Fixatives can Better Preserve Tissue Morphology than Formalin
title_full_unstemmed	Alcohol-Based Fixatives can Better Preserve Tissue Morphology than Formalin
title_sort	alcohol-based fixatives can better preserve tissue morphology than formalin
publisher	Sociedad Chilena de Anatomía
publishDate	2020
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022020000501371
work_keys_str_mv	AT haqueziaul alcoholbasedfixativescanbetterpreservetissuemorphologythanformalin AT rahmanasabur alcoholbasedfixativescanbetterpreservetissuemorphologythanformalin AT khanzahirulislam alcoholbasedfixativescanbetterpreservetissuemorphologythanformalin AT hussanmohammadtufazzal alcoholbasedfixativescanbetterpreservetissuemorphologythanformalin AT alammahmudul alcoholbasedfixativescanbetterpreservetissuemorphologythanformalin
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Alcohol-Based Fixatives can Better Preserve Tissue Morphology than Formalin

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