Rabbit Vomeronasal Organ-Derivered Cells Have Mesenchymal Profile and Neuronal Commitment

SUMMARY: The vomeronasal organ (VNO) is an accessory organ involved on the olfactory pathway, that detects pheromones and emits signals in order to modulate social and reproductive behavior. The VNO stem cells replace neurons throughout life. The aim of this study was to isolate and characterize cel...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Barreto,Rodrigo S. N, de-Oliveira,Franceliusa Delys, Matias,Gustavo de Sá Schiavo, Rodrigues,Marcio N, Carvalho,Rafael C, Franciolli,André Luis R, Fratini,Paula, Miglino,Maria Angelica
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2020
Materias:	Olfactory System Olfactory epithelium Olfactory bulb Neuronal stem cells
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022020000501463
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0717-95022020000501463
record_format	dspace
spelling	oai:scielo:S0717-950220200005014632021-06-10Rabbit Vomeronasal Organ-Derivered Cells Have Mesenchymal Profile and Neuronal CommitmentBarreto,Rodrigo S. Nde-Oliveira,Franceliusa DelysMatias,Gustavo de Sá SchiavoRodrigues,Marcio NCarvalho,Rafael CFranciolli,André Luis RFratini,PaulaMiglino,Maria Angelica Olfactory System Olfactory epithelium Olfactory bulb Neuronal stem cells SUMMARY: The vomeronasal organ (VNO) is an accessory organ involved on the olfactory pathway, that detects pheromones and emits signals in order to modulate social and reproductive behavior. The VNO stem cells replace neurons throughout life. The aim of this study was to isolate and characterize cells derived from the vomeronasal organ from New Zealand rabbits. Five male rabbits with 120 days were used for cell isolation and culture. Results: VNO-derived cells presented labelling for proliferation (PCNA), undifferentiated profile (Nanog), neuronal (GFAP), mesenchymal stem cells (CD73, CD90 and CD105 and Stro-1). Also, presence of cytoskeletal (Vimentin, b-tubulin and CK-18) and absence of hematopoietic markers (CD34, CD117 and CD45) both by immunofluorescence and flow cytometry. By PCR it was possible to verify the expression of some undifferentiated profile (Oct-4), neuronal (Nestin) and mesenchymal (CD73, CD105 and Vimentin) genes. Functionally, VNO-derived cells differentiate in vitro into adipocytes, osteocytes and chondrocytes, and presented no tumorigenic potential when injected to Balb/c nu/nu mice. In conclusion, the rabbit VNO-derived cells have a profile that could be supportive to VNO olfactory/neuroreceptor epithelium by delivering factors to epithelial turnover or even by differentiation into epithelial cells to replacement of commissural epithelium.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.38 n.5 20202020-10-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022020000501463en10.4067/S0717-95022020000501463
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Olfactory System Olfactory epithelium Olfactory bulb Neuronal stem cells
spellingShingle	Olfactory System Olfactory epithelium Olfactory bulb Neuronal stem cells Barreto,Rodrigo S. N de-Oliveira,Franceliusa Delys Matias,Gustavo de Sá Schiavo Rodrigues,Marcio N Carvalho,Rafael C Franciolli,André Luis R Fratini,Paula Miglino,Maria Angelica Rabbit Vomeronasal Organ-Derivered Cells Have Mesenchymal Profile and Neuronal Commitment
description	SUMMARY: The vomeronasal organ (VNO) is an accessory organ involved on the olfactory pathway, that detects pheromones and emits signals in order to modulate social and reproductive behavior. The VNO stem cells replace neurons throughout life. The aim of this study was to isolate and characterize cells derived from the vomeronasal organ from New Zealand rabbits. Five male rabbits with 120 days were used for cell isolation and culture. Results: VNO-derived cells presented labelling for proliferation (PCNA), undifferentiated profile (Nanog), neuronal (GFAP), mesenchymal stem cells (CD73, CD90 and CD105 and Stro-1). Also, presence of cytoskeletal (Vimentin, b-tubulin and CK-18) and absence of hematopoietic markers (CD34, CD117 and CD45) both by immunofluorescence and flow cytometry. By PCR it was possible to verify the expression of some undifferentiated profile (Oct-4), neuronal (Nestin) and mesenchymal (CD73, CD105 and Vimentin) genes. Functionally, VNO-derived cells differentiate in vitro into adipocytes, osteocytes and chondrocytes, and presented no tumorigenic potential when injected to Balb/c nu/nu mice. In conclusion, the rabbit VNO-derived cells have a profile that could be supportive to VNO olfactory/neuroreceptor epithelium by delivering factors to epithelial turnover or even by differentiation into epithelial cells to replacement of commissural epithelium.
author	Barreto,Rodrigo S. N de-Oliveira,Franceliusa Delys Matias,Gustavo de Sá Schiavo Rodrigues,Marcio N Carvalho,Rafael C Franciolli,André Luis R Fratini,Paula Miglino,Maria Angelica
author_facet	Barreto,Rodrigo S. N de-Oliveira,Franceliusa Delys Matias,Gustavo de Sá Schiavo Rodrigues,Marcio N Carvalho,Rafael C Franciolli,André Luis R Fratini,Paula Miglino,Maria Angelica
author_sort	Barreto,Rodrigo S. N
title	Rabbit Vomeronasal Organ-Derivered Cells Have Mesenchymal Profile and Neuronal Commitment
title_short	Rabbit Vomeronasal Organ-Derivered Cells Have Mesenchymal Profile and Neuronal Commitment
title_full	Rabbit Vomeronasal Organ-Derivered Cells Have Mesenchymal Profile and Neuronal Commitment
title_fullStr	Rabbit Vomeronasal Organ-Derivered Cells Have Mesenchymal Profile and Neuronal Commitment
title_full_unstemmed	Rabbit Vomeronasal Organ-Derivered Cells Have Mesenchymal Profile and Neuronal Commitment
title_sort	rabbit vomeronasal organ-derivered cells have mesenchymal profile and neuronal commitment
publisher	Sociedad Chilena de Anatomía
publishDate	2020
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022020000501463
work_keys_str_mv	AT barretorodrigosn rabbitvomeronasalorganderiveredcellshavemesenchymalprofileandneuronalcommitment AT deoliveirafranceliusadelys rabbitvomeronasalorganderiveredcellshavemesenchymalprofileandneuronalcommitment AT matiasgustavodesaschiavo rabbitvomeronasalorganderiveredcellshavemesenchymalprofileandneuronalcommitment AT rodriguesmarcion rabbitvomeronasalorganderiveredcellshavemesenchymalprofileandneuronalcommitment AT carvalhorafaelc rabbitvomeronasalorganderiveredcellshavemesenchymalprofileandneuronalcommitment AT franciolliandreluisr rabbitvomeronasalorganderiveredcellshavemesenchymalprofileandneuronalcommitment AT fratinipaula rabbitvomeronasalorganderiveredcellshavemesenchymalprofileandneuronalcommitment AT miglinomariaangelica rabbitvomeronasalorganderiveredcellshavemesenchymalprofileandneuronalcommitment
_version_	1718445167994732544

Rabbit Vomeronasal Organ-Derivered Cells Have Mesenchymal Profile and Neuronal Commitment

Ejemplares similares