A Double Fluorescence Chimeric Limb Regeneration Model Reveals Muscle Fiber Reconnection During Axolotl Limb Regeneration

SUMMARY: Axolotl limb regeneration is a fascinating characteristic that has attracted attention for several decades. Our previous studies on axolotl limb regeneration indicated that the satellite cells in the remnant muscles move distally into the blastema to regenerate new muscles that are separate...

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Autores principales:	Wang,Mu-Hui, Huang,Ting-Yu, Wu,Cheng-Han, Chiou,Ling-Ling, Lee,Hsuan-Shu
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2020
Materias:	Transgenic EGFP RFP Blastema transplantation Muscle fusion
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022020000501485
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spelling	oai:scielo:S0717-950220200005014852021-06-10A Double Fluorescence Chimeric Limb Regeneration Model Reveals Muscle Fiber Reconnection During Axolotl Limb RegenerationWang,Mu-HuiHuang,Ting-YuWu,Cheng-HanChiou,Ling-LingLee,Hsuan-Shu Transgenic EGFP RFP Blastema transplantation Muscle fusion SUMMARY: Axolotl limb regeneration is a fascinating characteristic that has attracted attention for several decades. Our previous studies on axolotl limb regeneration indicated that the satellite cells in the remnant muscles move distally into the blastema to regenerate new muscles that are separated by a gap from remnant muscles. Thereafter, the regenerative muscle fibers start to reconnect with remnant ones. In this study, the reconnection at the individual muscle fiber level was elucidated to test the hypothesis that this reconnection happens synchronously among involved muscles. Three pairs of EGFP+ mid-bud stage blastemas were transplanted onto freshly amputated stumps of RFP+ axolotls at the same thigh position to generate double fluorescence chimeric regenerative hindlimbs. These regenerative limbs were harvested very late far beyond they had reached the late differentiation stage. Fluorescence imaging of these limbs in cross sections revealed that in the proximal remnant part of the muscle fiber, reconnection occurred at a different pace among the muscles. In the major thigh muscle gracilis, the reconnection started from the periphery before it was completed. Furthermore, RFP+ muscle fibers contributed to muscle regeneration in the distal regenerative parts. Intriguingly, this red cell contribution was limited to ventral superficial muscles of the calf. This kind of double fluorescence chimeric limb regeneration model may help increase the understanding of the patterning of axolotl limb regeneration in late stages.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.38 n.5 20202020-10-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022020000501485en10.4067/S0717-95022020000501485
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Transgenic EGFP RFP Blastema transplantation Muscle fusion
spellingShingle	Transgenic EGFP RFP Blastema transplantation Muscle fusion Wang,Mu-Hui Huang,Ting-Yu Wu,Cheng-Han Chiou,Ling-Ling Lee,Hsuan-Shu A Double Fluorescence Chimeric Limb Regeneration Model Reveals Muscle Fiber Reconnection During Axolotl Limb Regeneration
description	SUMMARY: Axolotl limb regeneration is a fascinating characteristic that has attracted attention for several decades. Our previous studies on axolotl limb regeneration indicated that the satellite cells in the remnant muscles move distally into the blastema to regenerate new muscles that are separated by a gap from remnant muscles. Thereafter, the regenerative muscle fibers start to reconnect with remnant ones. In this study, the reconnection at the individual muscle fiber level was elucidated to test the hypothesis that this reconnection happens synchronously among involved muscles. Three pairs of EGFP+ mid-bud stage blastemas were transplanted onto freshly amputated stumps of RFP+ axolotls at the same thigh position to generate double fluorescence chimeric regenerative hindlimbs. These regenerative limbs were harvested very late far beyond they had reached the late differentiation stage. Fluorescence imaging of these limbs in cross sections revealed that in the proximal remnant part of the muscle fiber, reconnection occurred at a different pace among the muscles. In the major thigh muscle gracilis, the reconnection started from the periphery before it was completed. Furthermore, RFP+ muscle fibers contributed to muscle regeneration in the distal regenerative parts. Intriguingly, this red cell contribution was limited to ventral superficial muscles of the calf. This kind of double fluorescence chimeric limb regeneration model may help increase the understanding of the patterning of axolotl limb regeneration in late stages.
author	Wang,Mu-Hui Huang,Ting-Yu Wu,Cheng-Han Chiou,Ling-Ling Lee,Hsuan-Shu
author_facet	Wang,Mu-Hui Huang,Ting-Yu Wu,Cheng-Han Chiou,Ling-Ling Lee,Hsuan-Shu
author_sort	Wang,Mu-Hui
title	A Double Fluorescence Chimeric Limb Regeneration Model Reveals Muscle Fiber Reconnection During Axolotl Limb Regeneration
title_short	A Double Fluorescence Chimeric Limb Regeneration Model Reveals Muscle Fiber Reconnection During Axolotl Limb Regeneration
title_full	A Double Fluorescence Chimeric Limb Regeneration Model Reveals Muscle Fiber Reconnection During Axolotl Limb Regeneration
title_fullStr	A Double Fluorescence Chimeric Limb Regeneration Model Reveals Muscle Fiber Reconnection During Axolotl Limb Regeneration
title_full_unstemmed	A Double Fluorescence Chimeric Limb Regeneration Model Reveals Muscle Fiber Reconnection During Axolotl Limb Regeneration
title_sort	double fluorescence chimeric limb regeneration model reveals muscle fiber reconnection during axolotl limb regeneration
publisher	Sociedad Chilena de Anatomía
publishDate	2020
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022020000501485
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A Double Fluorescence Chimeric Limb Regeneration Model Reveals Muscle Fiber Reconnection During Axolotl Limb Regeneration

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